RhoF Antibody

Western Blot: RhoF Antibody [NBP2-20157] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with RHOF antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: RhoF Antibody [NBP2-20157] - Immunohistochemical analysis of paraffin-embedded colon ca, using antibody at 1:500 dilution.

Western Blot: RhoF Antibody [NBP2-20157] - Sample (30 ug of whole cell lysate) A: U87-MG 12% SDS PAGE gel, diluted at 1:500.

Product Discontinued

RhoF Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human RHOF. The exact sequence is proprietary.
• Localization: Cell membrane; Lipid-anchor; Cytoplasmic side (Potential); Cytoplasm; cytoskeleton
• Predicted Species: Porcine (97%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RHOF
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (80%), Chicken (82%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for RhoF Antibody

• FLJ20247
• ras homolog gene family, member F (in filopodia)
• Rho family GTPase Rif
• Rho in filopodia
• rho-related GTP-binding protein RhoF
• RIFARHF

Background

Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: RHOF
• Uniprot:
  • Q9HBH0(Human)