Biological Strategies: Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Immunoprecipitation: NCAPG2 Antibody [NB100-1813] - Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared ...read more
The immunogen recognized by this antibody maps to a region between residue 1100 and the C-terminus (residue 1143) of human Chromosome-Associated Protein G2 using the numbering given in entry AAH43404.1 (GeneID 54892).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Use in ICC/IF reported in scientific literature (PMID: 30143891). Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
131 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NB100-1813 in the following applications:
Leucine zipper protein 5CAP-G2hCAP-G2condensin-2 complex subunit G2
more than blood homolog
Non-SMC condensin II complex subunit G2
non-SMC condensin II complex, subunit G2
CAP-G2 is a subunit of the heteropentameric condensin II complex. Condensin participates in the condensation of chromosomes during cell division by facilitating positive DNA supercoiling and knotting by topoisomerase I and II and proper segregation of sister chromatids. CAP-G2 is a non-SMC regulatory subunit of the condensin complex. Alternate names for CAP-G include condensin-II complex subunit G2, chromosome-associated protein G2, hCAP-G2, CAP-G2, Non-SMC condensin II complex subunit G2, leucine zipper protein 5, NCAPG2, LUZP5, MTB, and FLJ20311.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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