RHAMM/CD168 Antibody

Western Blot: CD168 Antibody [NBP2-15781] - Sample (20 ug of whole cell lysate) A: human ESC 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: RHAMM/CD168 Antibody [NBP2-15781] - RHAMM/CD168 Antibody detects RHAMM/CD168 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RHAMM/CD168 Antibody dilution: 1:250. Scale bar = 100 um.

Immunohistochemistry-Paraffin: CD168 Antibody [NBP2-15781] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Immunoprecipitation: RHAMM/CD168 Antibody [NBP2-15781] - Immunoprecipitation of RHAMM protein from 293T whole cell extracts using 5 ug of RHAMM antibody [N1N2], N-term. Western blot analysis was performed using RHAMM antibody [N1N2], N-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

RHAMM/CD168 Antibody Summary

• Description: Novus Biologicals Rabbit RHAMM/CD168 Antibody (NBP2-15781) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RHAMM/CD168. The exact sequence is proprietary.
• Localization: Cell surface; Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HMMR
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for RHAMM/CD168 Antibody

• CD168 antigen
• CD168
• HMMR
• hyaluronan mediated motility receptor
• hyaluronan-mediated motility receptor (RHAMM)
• IHABP
• Intracellular hyaluronic acid-binding protein
• MGC119494
• Receptor for hyaluronan-mediated motility
• RHAMM
• RHAMMMGC119495

Background

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HMMR
• Uniprot:
  • O75330(Human)