RHAMM/CD168 Antibody (PSH02-16)

Western Blot: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Western blot analysis of RHAMM/CD168 on different lysates with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: SW480 cell lysate Lane 3: HepG2 cell lysate Lane 4: Raji cell lysate Lane 5: MCF7 cell lysate Lane 6: SK-Br-3 cell lysate Lane 7: HeLa cell lysate Lane 8: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 1 minute 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32129) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32129) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Immunocytochemistry analysis of HeLa cells labeling RHAMM/CD168 with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-RHAMM/CD168 antibody (NBP3-32129) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: RHAMM/CD168 Antibody (PSH02-16) [NBP3-32129] - Flow cytometric analysis of HeLa cells labeling RHAMM/CD168. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32129, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: PSH02-16
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RHAMM/CD168 Antibody (PSH02-16) Summary

• Description: Novus Biologicals Rabbit RHAMM/CD168 Antibody (PSH02-16) (NBP3-32129) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human RHAMM/CD168 aa 1-350 / 724 (O75330). (Uniprot: O75330)
• Localization: Cell surface, Cytoplasm, cytoskeleton, spindle.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HMMR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Theoretical MW: 84 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RHAMM/CD168 Antibody (PSH02-16)

• CD168 antigen
• CD168
• HMMR
• hyaluronan mediated motility receptor
• hyaluronan-mediated motility receptor (RHAMM)
• IHABP
• Intracellular hyaluronic acid-binding protein
• MGC119494
• Receptor for hyaluronan-mediated motility
• RHAMM
• RHAMMMGC119495

Background

The protein encoded by the CD168 gene is involved in cell motility. It is expressed in breast tissue and together with otherproteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer.Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HMMR