RHAMM/CD168 Antibody (2F2C9) - BSA Free

Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against HEK293 (1) and CD168 (AA: 306-497)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437AF488 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Western Blot: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis using CD168 mAb against human CD168 (AA: 306-497) recombinant protein. (Expected MW is 48.3 kDa)

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Analysis of Hela cells using CD168 mouse mAb (green) and negative control (red).

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on A549 cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Flow Cytometry: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - An intracellular stain was performed on Jurkat cells with RHAMM/CD168 Antibody (2F2C9) NBP2-52437PE and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

ELISA: RHAMM/CD168 Antibody (2F2C9) [NBP2-52437] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 2F2C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RHAMM/CD168 Antibody (2F2C9) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human RHAMM/CD168 (AA: 306-497) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HMMR
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 84.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RHAMM/CD168 Antibody (2F2C9) - BSA Free

• CD168 antigen
• CD168
• HMMR
• hyaluronan mediated motility receptor
• hyaluronan-mediated motility receptor (RHAMM)
• IHABP
• Intracellular hyaluronic acid-binding protein
• MGC119494
• Receptor for hyaluronan-mediated motility
• RHAMM
• RHAMMMGC119495

Background

The protein encoded by the CD168 gene is involved in cell motility. It is expressed in breast tissue and together with otherproteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer.Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. (provided byRefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RHAMM/CD168 Antibody (NBP2-52437)(1)

Publication using NBP2-52437

• Shea DJ, Li YW, Stebe KJ, Konstantopoulos K. E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid FASEB J. 2017-08-01 [PMID: 28765175] (FLOW)

Bioinformatics

• Gene Symbol: HMMR