RFXANK Antibody (OTI3B4)

Western Blot: RFXANK Antibody (OTI3B4) [NBP2-45571] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK .

Immunofluorescence: RFXANK Antibody (OTI3B4) [NBP2-45571] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY RFXANK.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI3B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

RFXANK Antibody (OTI3B4) Summary

• Description: Novus Biologicals Mouse RFXANK Antibody (OTI3B4) (NBP2-45571) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RFXANK(NP_604389) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RFXANK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 25.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RFXANK Antibody (OTI3B4)

• ANKRA1Regulatory factor X subunit B
• ankyrin repeat-containing regulatory factor X-associated protein
• BLS
• DNA-binding protein RFXANK
• F14150_1
• MGC138628
• regulatory factor X-associated ankyrin-containing proteinAnkyrin repeat family A protein 1
• RFXB
• RFX-BRFX-Bdelta4

Background

RFX-B is the gene responsible for the bare lymphocyte syndrome (BLS) which is characterized by a lack of MHC class II transcription. Subsequently, there is a lack of B and T cell responses to foreign antigens. RFX-B is an MHC class II transcription factor that is defective in BLS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RFXANK