Reelin Antibody - BSA Free

Western Blot: Reelin Antibody [NBP3-13177] - Various tissue extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Reelin antibody (NBP3-13177) diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Reelin Antibody [NBP3-13177] - Reelin antibody detects Reelin protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Reelin protein stained by Reelin antibody (NBP3-13177) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: Reelin Antibody [NBP3-13177] - Reelin antibody detects Reelin protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebral cortex. Green: Reelin stained by Reelin antibody (NBP3-13177) diluted at 1:250. Blue: Fluoroshield with DAPI.

• Reactivity: Mu, Rt, Hu, Bv, Ch, Sh
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Reelin Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of mouse Reelin. The exact sequence is proprietary.
• Predicted Species: Human (97%), Sheep (98%), Chicken (91%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RELN
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Protein A purified

Alternate Names for Reelin Antibody - BSA Free

• EC 3.4.21
• EC 3.4.21.-
• Reeler
• Reelin
• RELN
• RL
• RLPRO1598

Background

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RELN