Recombinant Zebrafish VEGF 165 Protein, CF

• Reactivity: Ze
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Zebrafish VEGF 165 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 20-60 ng/mL.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived zebrafish VEGF protein
  Ala24-Arg188
• Accession #: O73682.1
• N-terminal Sequence: Ala24
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: vegfaa
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.2 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 22 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Zebrafish VEGF 165 Protein, CF

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues. In human, at least eight alternate splice isoforms of VEGF-A, ranging from 206 amino acids (aa) to 121 aa in length, are known. In zebrafish, two VEGF isoforms, a 165 aa and a 121 aa isoform, have been reported. Mature zebrafish VEGF₁₆₅ shares 64%, 62% and 62% aa sequence identity with frog, human, and mouse VEGF₁₆₅, respectively. There are two tyrosine kinase receptors for VEGF reported in mammals termed VEGF R1 and VEGF R2/FLK-1. One receptor has been identified in zebrafish(FLK-1), and this may actually represent the orthologue to the early common ancestor for mammalian VEGF R1 and R2. All receptors are type I transmembrane proteins that show seven immunoglobulin-like domains extracellularly and a split kinase domain intracellularly. In addition to the tyrosine kinase receptors, neuropilin-1 (NRP-1) has been reported to be a coreceptor for VEGF binding. It is proposed that the presence of NRP-1 lowers the concentration of VEGF necessary for activation of VEGF R2. NRP-1 has been reported in both zebrafish and human. VEGF regulates multiple biological functions in endothelial cells, including cell proliferation, migration and survival. These functions of VEGF are mediated partly through the induction of nitric oxide and prostacyclin, as well as upregulation of metalloproteinases. Together with other vascular-specific growth factors such as the Angiopoietins, VEGF have separate but complementary roles in angiogenesis and vasculogenesis (1‑7).

1. Laing, D. et al. (1998) Biochem. Biophys. Acta 1397:14.
2. Laing, D. and R. Ge (1998) GenBank Accession # AAC14713.
3. Laio, W. et al. (1997) Development 124:381.
4. Lee, P. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10470.
5. Thurston, G. (2002) J. Anat. 200:575.
6. Zachary, I. and G. Gliki (2001) Cardiovasc. Res. 49:568.
7. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.

Publications for VEGF (1247-ZV/CF)(2)

Publications using 1247-ZV/CF

• J Müller-Dei, P Schröder, L Beverly-St, R Hiss, J Fiedler, J Nyström, T Thum, H Haller, M Schiffer Overexpression of preeclampsia induced microRNA-26a-5p leads to proteinuria in zebrafish Sci Rep, 2018-02-26;8(1):3621. 2018-02-26 [PMID: 29483572] (In Vivo, Zebrafish)
• Bovenkamp DE, Goishi K, Bahary N, Davidson AJ, Zhou Y, Becker T, Becker CG, Zon LI, Klagsbrun M Expression and mapping of duplicate neuropilin-1 and neuropilin-2 genes in developing zebrafish. Gene Expr. Patterns, 2004-07-01;4(4):361-70. 2004-07-01 [PMID: 15183302] (Binding Assay, Zebrafish)

FAQs for VEGF (1247-ZV/CF). (Showing 1 - 1 of 1 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Bioinformatics

• Gene Symbol: vegfaa
• Uniprot:
  • O73682.1()