Recombinant Rat VEGF 164 Protein

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1 μg/lane of Recombinant Rat VEGF164 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 54 kDa, respectively.
Recombinant Rat VEGF164 (Catalog # 564‑RV) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner. The ED50 is 0.75-3.75 ng/mL.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity

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Recombinant Rat VEGF 164 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 0.75‑3.75 ng/mL.
Source
Mouse myeloma cell line, NS0-derived rat VEGF protein
Ala27-Arg190
Accession #
N-terminal Sequence
Ala27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Vegfa
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
25 kDa, reducing conditions
Publications
Read Publications using
564-RV in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat VEGF 164 Protein

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by a cysteine-knot structure formed by eight conserved cysteine residues (4). Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in rat and mouse (2, 4). Isoforms other than VEGF120 and VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). Human VEGF165 binds the semaphorin receptor, neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

  1. Conn, G. et al. (1990) J. Biol. Chem. 87:2628.
  2. Ishii, H. et al. (2001) Arch. Oral Biol. 46:77.
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
  4. Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853.
  5. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  6. Weis, S.M. & D.A. Cheresh (2005) Nature 437:497.
  7. Thurston, G. (2002) J. Anat. 200:575.
  8. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  9. Angelo, L.S. & R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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564-RV
Species: Rt
Applications: Bioactivity

Publications for VEGF (564-RV)(23)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Xenopus.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA (Standard), In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(9)
ELISA (Standard)
(1)
In Vivo
(12)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(18)
N/A
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using 564-RV Applications Species
GA Rodrigues, M Mason, LA Christie, C Hansen, LM Hernandez, J Burke, KA Luhrs, TC Hohman Functional Characterization of Abicipar-Pegol, an Anti-VEGF DARPin Therapeutic That Potently Inhibits Angiogenesis and Vascular Permeability Invest. Ophthalmol. Vis. Sci., 2018;59(15):5836-5846. 2018 [PMID: 30535424] (Bioassay, Human) Bioassay Human
C Chang, K Li, S Jiang, B Li, L Cao, P Wang Downregulation of TRPC6 expression is a critical molecular event during FK506 treatment for overactive bladder Cell Calcium, 2018;77(0):8-19. 2018 [PMID: 30476735] (Bioassay, Rat) Bioassay Rat
M Li, Y Cui, W He, X Deng, Y Wang, M Cai, Y Wang, J Pei, X Mei, P Wu Effects of Triple-Mutated Hypoxia-Inducible Factor-1? on Angiogenesis and Cardiac Function Improvement in Rats with Myocardial Infarction Cell. Physiol. Biochem., 2018;50(6):2329-2340. 2018 [PMID: 30423574] (In Vivo, Rat) In Vivo Rat
M Vitacolonn In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue Cell Tissue Bank, 2016;0(0):. 2016 [PMID: 28004288] (Bioassay, Rat) Bioassay Rat
Angiogenic microspheres promote neural regeneration and motor function recovery after spinal cord injury in rats Sci Rep, 2016;6(0):33428. 2016 [PMID: 27641997] (In Vivo, Rat) In Vivo Rat
Caspase-3 dependent nitrergic neuronal apoptosis following cavernous nerve injury is mediated via RhoA and ROCK activation in major pelvic ganglion Sci Rep, 2016;6(0):29416. 2016 [PMID: 27388816] (Bioassay, Rat) Bioassay Rat
Weinkopff T, Mackenzie C, Eversole R, Lammie P Filarial excretory-secretory products induce human monocytes to produce lymphangiogenic mediators. PLoS Negl Trop Dis, 2014;8(7):e2893. 2014 [PMID: 25010672] (In Vivo, Rat) In Vivo Rat
Sjoqvist S, Jungebluth P, Lim M, Haag J, Gustafsson Y, Lemon G, Baiguera S, Burguillos M, Del Gaudio C, Rodriguez A, Sotnichenko A, Kublickiene K, Ullman H, Kielstein H, Damberg P, Bianco A, Heuchel R, Zhao Y, Ribatti D, Ibarra C, Joseph B, Taylor D, Macchiarini P Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nat Commun, 2014;5(0):3562. 2014 [PMID: 24736316] (Bioassay, Rat) Bioassay Rat
Trichonas G, Manola A, Morizane Y, Thanos A, Koufomichali X, Papakostas TD, Montezuma S, Young L, Miller JW, Gragoudas E, Vavvas D A novel nonradioactive method to evaluate vascular barrier breakdown and leakage. Invest. Ophthalmol. Vis. Sci., 2010;51(3):1677-82. 2010 [PMID: 19875655] (In Vivo, Rat) In Vivo Rat
Hu J, Dong A, Fernandez-Ruiz V, Shan J, Kawa M, Martinez-Anso E, Prieto J, Qian C Blockade of Wnt signaling inhibits angiogenesis and tumor growth in hepatocellular carcinoma. Cancer Res., 2009;69(17):6951-9. 2009 [PMID: 19690140] (Bioassay, Mouse) Bioassay Mouse
Show All 23 Publications.

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FAQs for VEGF (564-RV). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Blogs on VEGF. Showing 1-10 of 19 blog posts - Show all blog posts.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol Vegfa
Uniprot