Recombinant Rat Leptin Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat Leptin Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human Leptin R. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived rat Leptin/OB protein
Val22-Cys167, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Lep
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
16.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
598-LP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI, pH 8.0.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat Leptin Protein, CF

  • FLJ94114
  • LEP
  • leptin (murine obesity homolog)
  • leptin (obesity homolog, mouse)
  • Leptin
  • OB
  • Obese protein
  • obese, mouse, homolog of
  • Obesity factor
  • OBOBS

Background

Leptin is a identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of Leptin have been found to be obese and diabetic and to have reduced activity, metabolism, and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. Rat Leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. Rat Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue.

A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines (1 - 3). The possible roles of Leptin in body weight regulation, hematopoiesis and reproduction are being investigated.

  1. Considine, R. and Caro, J. (1996) Clinical Chemistry 42:843.
  2. Tartaglia, L.A. et al., (1995) Cell 83:1263.
  3. Cioffi, J.A. et al. (1996) Nature Medicine 2:585.

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Publications for Leptin/OB (598-LP)(15)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: Bioassay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(1)
In Vivo
(11)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(1)
Rat
(13)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 598-LP Applications Species
T Murata, K Asanuma, N Ara, K Iijima, W Hatta, S Hamada, N Asano, T Koike, A Imatani, A Masamune, T Shimosegaw Leptin Aggravates Reflux Esophagitis by Increasing Tissue Levels of Macrophage Migration Inhibitory Factor in Rats Tohoku J. Exp. Med., 2018;245(1):45-53. 2018 [PMID: 29760351] (In Vivo, Rat) In Vivo Rat
MA Abraham, M Rasti, PV Bauer, TKT Lam Leptin enhances hypothalamic LDHA-dependent glucose sensing to lower glucose production in high-fat fed rats J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29374061] (In Vivo, Rat) In Vivo Rat
YC Han, B Ma, S Guo, M Yang, LJ Li, SJ Wang, J Tan Leptin regulates disc cartilage endplate degeneration and ossification through activation of the MAPK-ERK signalling pathway in�vivo and in�vitro J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 29372627] (In Vivo, Rat) In Vivo Rat
VE Vargas, S Gurung, B Grant, K Hyatt, K Singleton, SM Myers, D Saunders, C Njoku, R Towner, DA Myers Gestational hypoxia disrupts the neonatal leptin surge and programs hyperphagia and obesity in male offspring in the Sprague-Dawley rat PLoS ONE, 2017;12(9):e0185272. 2017 [PMID: 28957383] (In Vivo, Rat) In Vivo Rat
Liu-Jin Luo Leptin Attenuates the Contractile Function of Adult Rat Cardiomyocytes Involved in Oxidative Stress and Autophagy Acta Cardiol Sin, 2016;32(6):723-730. 2016 [PMID: 27899860] (Bioassay, Rat) Bioassay Rat
Bhavna N Desai Fourth ventricle leptin infusions dose dependently activate hypothalamic signal transducer and activator of transcription 3 (STAT3) Am. J. Physiol. Endocrinol. Metab., 2016;0(0):ajpendo.00343. 2016 [PMID: 27802966] (In Vivo, Rat) In Vivo Rat
Thieme K, Oliveira-Souza M Renal hemodynamic and morphological changes after 7 and 28 days of leptin treatment: the participation of angiotensin II via the AT1 receptor. PLoS ONE, 2015;10(3):e0122265. 2015 [PMID: 25793389] (Rat) Rat
Jamroz-Wisniewska A, Gertler A, Solomon G, Wood M, Whiteman M, Beltowski J Leptin-induced endothelium-dependent vasorelaxation of peripheral arteries in lean and obese rats: role of nitric oxide and hydrogen sulfide. PLoS ONE, 2014;9(1):e86744. 2014 [PMID: 24475175] (In Vivo, Rat) In Vivo Rat
Vaill M, Desai B, Harris R Blockade of the cerebral aqueduct in rats provides evidence of antagonistic leptin responses in the forebrain and hindbrain. Am J Physiol Endocrinol Metab, 2014;306(4):E414-23. 2014 [PMID: 24347057] (In Vivo, Rat) In Vivo Rat
Huang W, Metlakunta A, Dedousis N, Ortmeyer HK, Stefanovic-Racic M, O'Doherty RM Leptin augments the acute suppressive effects of insulin on hepatic very low-density lipoprotein production in rats. Endocrinology, 2009;150(5):2169-74. 2009 [PMID: 19147673]
Show All 15 Publications.

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Bioinformatics

Gene Symbol Lep
Entrez
Uniprot