Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF

Images

 
There are currently no images for ICAM-1/CD54 (583-IC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.

When 5 x 104 cells/well are added to rrICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), >20% will adhere after 1 hour incubation at 37 °C.

Also supports adhesion (40-70%) of PMA treated rat Nb-211 cells when coated at 12.5 µg/mL.
Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived rat ICAM-1/CD54 protein
Rat ICAM-1
(Gln28 - Thr493)
Accession # Q00238
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln28 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Icam1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
78 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-125 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 400 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Customers Who Viewed This Item Also Viewed...

AF1730
Species: Hu
Applications: WB, Flow, AdBlk, CyTOF-ready, ICC
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
BBA16
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-84550
Species: Hu
Applications: WB, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF1008
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
NB110-89474
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Single Cell Western
MAB140
Species: Hu
Applications: Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP2-22542
Species: Hu
Applications: WB, B/N, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-56583
Species: Hu
Applications: WB, Flow, IHC-P
NBP2-25208
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, KO
583-IC
Species: Rt
Applications: Bioactivity

Publications for ICAM-1/CD54 (583-IC) (0)

There are no publications for ICAM-1/CD54 (583-IC).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ICAM-1/CD54 (583-IC) (0)

There are no reviews for ICAM-1/CD54 (583-IC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for ICAM-1/CD54 (583-IC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

CD11b Antibody
NB110-89474

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Rat ICAM-1/CD54 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol Icam1
Entrez
Uniprot