Recombinant Rat CNTF Protein, CF

• Reactivity: Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Rat CNTF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 3-15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED₅₀ for this effect is 0.1-0.3 ng/mL.
• Source: E. coli-derived rat CNTF protein
  Ala2-Met200
• Accession #: P20294.1
• N-terminal Sequence: Ala2
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cntf
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 22.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CNTF Protein, CF

• ciliary neurotrophic factor
• CNTF
• HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems' cytokine catalog.

Publications for CNTF (557-NT/CF)(36)

Publications using 557-NT/CF

• EF Cohn, BLL Clayton, M Madhavan, S Yacoub, Y Federov, K Paul-Fried, TJ Shafer, PJ Tesar Pervasive environmental chemicals impair oligodendrocyte development bioRxiv : the preprint server for biology, 2023-02-12;0(0):. 2023-02-12 [PMID: 36798415] (Bioassay, Mouse)
• Y Tang, DJ Kwiatkowsk, EP Henske Midkine expression by stem-like tumor cells drives persistence to mTOR inhibition and an immune-suppressive microenvironment Nature Communications, 2022-08-26;13(1):5018. 2022-08-26 [PMID: 36028490] (Bioassay, Mouse)
• S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 2022-08-25;13(1):5003. 2022-08-25 [PMID: 36008413] (Bioassay, Mouse)
• G D'Este, M Stazi, S Negro, A Megighian, F Lista, O Rossetto, C Montecucco, M Rigoni, M Pirazzini Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration International Journal of Molecular Sciences, 2022-01-21;23(3):. 2022-01-21 [PMID: 35163106] (Bioassay, Rat)
• KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020-10-21;0(0):. 2020-10-21 [PMID: 33091368] (Bioassay, Mouse)
• KM Collura, J Niu, SS Sanders, A Montersino, SM Holland, GM Thomas The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem., 2020-09-21;0(0):. 2020-09-21 [PMID: 32958558] (Bioassay, Rat)
• F Bucher, E Aguilar, KV Marra, J Rapp, J Arnold, S Diaz-Aguil, C Lange, H Agostini, G Schlunck, A Stahl, M Friedlande CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10 Invest. Ophthalmol. Vis. Sci., 2020-08-03;61(10):20. 2020-08-03 [PMID: 32780864] (In Vivo, Mouse)
• C Soulard, C Salsac, K Mouzat, C Hilaire, J Roussel, A Mezghrani, S Lumbroso, C Raoul, F Scamps Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis Cell Rep, 2020-02-25;30(8):2581-2593.e7. 2020-02-25 [PMID: 32101737] (Bioassay, Mouse)
• H Munezane, H Oizumi, T Wakabayash, S Nishio, T Hirasawa, T Sato, A Harada, T Yoshida, T Eguchi, Y Yamanashi, T Hashimoto, T Iwatsubo Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder Cell Rep, 2019-12-24;29(13):4362-4376.e6. 2019-12-24 [PMID: 31875546] (Cell Culture, Mouse)
• H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019-07-10;0(0):. 2019-07-10 [PMID: 31292217] (Cell Culture, Rat)
• N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019-03-14;9(1):4515. 2019-03-14 [PMID: 30872638] (Bioassay, Mouse)
• C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019-01-29;26(5):1143-1156.e5. 2019-01-29 [PMID: 30699345] (Bioassay, Mouse)
• J Di Pierdom, R Scholz, FJ Valiente-S, MC Sánchez-Mi, M Vidal-Sanz, T Langmann, M Agudo-Barr, D García-Ayu, MP Villegas-P Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration Invest. Ophthalmol. Vis. Sci., 2018-09-04;59(11):4392-4403. 2018-09-04 [PMID: 30193320] (In Vivo, Rat)
• KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018-05-22;9(6):596. 2018-05-22 [PMID: 29789529] (Bioassay, Mouse)
• R Rajagopal, S Zhang, X Wei, T Doggett, S Adak, J Enright, V Shah, G Ling, S Chen, J Yoshino, FF Hsu, CF Semenkovic Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision JCI Insight, 2018-01-11;3(1):. 2018-01-11 [PMID: 29321376] (Bioassay, Rat)
• SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins Front Pharmacol, 2017-02-23;8(0):73. 2017-02-23 [PMID: 28280466] (Bioassay, Mouse)
• S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493. 2017-03-07 [PMID: 28270618] (Bioassay, Mouse)
• CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 2016-08-01;57(10):4017-26. 2016-08-01 [PMID: 27494343] (Bioassay, Human)
• A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016-05-25;0(0):. 2016-05-25 [PMID: 27226316] (Bioassay, Rat)
• Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 2015-06-17;10(6):e0129944. 2015-06-17 [PMID: 26083449] (Bioassay, Mouse)
• Hong S, Song M Signal transducer and activator of transcription-3 maintains the stemness of radial glia at mid-neurogenesis. J Neurosci, 2015-01-21;35(3):1011-23. 2015-01-21 [PMID: 25609618] (Bioassay, Mouse)
• Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 2014-06-11;33(14):1582-98. 2014-06-11 [PMID: 24920579] (Bioassay, Mouse)
• Elsaeidi , Fairouz, Bemben , Michael, Zhao , Xiao-Fen, Goldman , Daniel Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci, 2014-02-12;34(7):2632-44. 2014-02-12 [PMID: 24523552] (Bioassay, Zebrafish)
• Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 2013-12-27;7(0):263. 2013-12-27 [PMID: 24409114] (In Vivo, Mouse)
• Dubovy P, Raska O, Klusakova I, Stejskal L, Celakovsky P, Haninec P Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy. BMC Neurosci, 2011-06-22;12(0):58. 2011-06-22 [PMID: 21696588] (In Vivo, Rat)
• Krencik R, Weick JP, Liu Y, Zhang ZJ, Zhang SC Specification of transplantable astroglial subtypes from human pluripotent stem cells. Nat. Biotechnol., 2011-05-22;29(6):528-34. 2011-05-22 [PMID: 21602806] (Bioassay, Human)
• Kent TL, Glybina IV, Abrams GW, Iezzi R Chronic intravitreous infusion of ciliary neurotrophic factor modulates electrical retinal stimulation thresholds in the RCS rat. Invest. Ophthalmol. Vis. Sci., 2008-01-01;49(1):372-9. 2008-01-01 [PMID: 18172115] (In Vivo, Rat)
• McGill TJ, Prusky GT, Douglas RM, Yasumura D, Matthes MT, Nune G, Donohue-Rolfe K, Yang H, Niculescu D, Hauswirth WW, Girman SV, Lund RD, Duncan JL, Lavail MM Intraocular CNTF reduces vision in normal rats in a dose-dependent manner. Invest. Ophthalmol. Vis. Sci., 2007-12-01;48(12):5756-66. 2007-12-01 [PMID: 18055829] (In Vivo, Rat)
• Ko ML, Liu Y, Dryer SE, Ko GY The expression of L-type voltage-gated calcium channels in retinal photoreceptors is under circadian control. J. Neurochem., 2007-08-07;103(2):784-92. 2007-08-07 [PMID: 17683482] (Bioassay, Chicken)
• Nakanishi M, Niidome T, Matsuda S, Akaike A, Kihara T, Sugimoto H Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur. J. Neurosci., 2007-02-01;25(3):649-58. 2007-02-01 [PMID: 17328769] (Bioassay, Rat)
• Janoschek R, Plum L, Koch L, Munzberg H, Diano S, Shanabrough M, Muller W, Horvath TL, Bruning JC gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor. Proc. Natl. Acad. Sci. U.S.A., 2006-07-03;103(28):10707-12. 2006-07-03 [PMID: 16818888] (In Vivo, Mouse)
• Hapner SJ, Nielsen KM, Chaverra M, Esper RM, Loeb JA, Lefcort F NT-3 and CNTF exert dose-dependent, pleiotropic effects on cells in the immature dorsal root ganglion: neuregulin-mediated proliferation of progenitor cells and neuronal differentiation. Dev. Biol., 2006-05-19;297(1):182-97. 2006-05-19 [PMID: 16784738] (Bioassay, Avian)
• Murata T, Tsuboi M, Hikita K, Kaneda N Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J. Biol. Chem., 2006-06-13;281(32):22503-16. 2006-06-13 [PMID: 16772303] (Bioassay, Mouse)
• Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 2005-05-25;16(7):1993-2002. 2005-05-25 [PMID: 15917337] (Bioassay, Rat)
• Fischer AJ, Omar G, Eubanks J, McGuire CR, Dierks BD, Reh TA Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage. Mol. Vis., 2004-12-22;10(0):973-86. 2004-12-22 [PMID: 15623987] (In Vivo, Chicken)
• Mizisin AP, Vu Y, Shuff M, Calcutt NA Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats. Diabetes, 2004-07-01;53(7):1807-12. 2004-07-01 [PMID: 15220205] (In Vivo, Rat)

Bioinformatics

• Gene Symbol: Cntf
• Uniprot:
  • P20294.1()