Recombinant Rat CNTF Protein, CF

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Summary
Reactivity RtSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Rat CNTF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3-15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 0.1-0.3 ng/mL.
Source
E. coli-derived rat CNTF protein
Ala2-Met200
Accession #
N-terminal Sequence
Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
Cntf
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
557-NT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CNTF Protein, CF

  • ciliary neurotrophic factor
  • CNTF
  • HCNTF

Background

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems' cytokine catalog.

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Publications for CNTF (557-NT/CF)(26)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian, Chicken, Zebrafish.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(17)
Cell Culture
(1)
In Vivo
(7)
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Human
(2)
Mouse
(10)
Rat
(9)
Avian
(1)
Chicken
(2)
Zebrafish
(1)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 557-NT/CF Applications Species
H Lindahl, AO Guerreiro-, SK Bedri, M Linnerbaue, M Lindén, N Abdelmagid, K Tandre, C Hollins, L Irving, C Glover, C Jones, L Alfredsson, L Rönnblom, I Kockum, M Khademi, M Jagodic, T Olsson IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 2019;0(0):. 2019 [PMID: 31292217] (Cell Culture, Rat) Cell Culture Rat
N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup The Parkinson&#039;s disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 2019;9(1):4515. 2019 [PMID: 30872638] (Bioassay, Mouse) Bioassay Mouse
C Wu, ME Watts, LL Rubin MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 2019;26(5):1143-1156.e5. 2019 [PMID: 30699345] (Bioassay, Mouse) Bioassay Mouse
J Di Pierdom, R Scholz, FJ Valiente-S, MC Sánchez-Mi, M Vidal-Sanz, T Langmann, M Agudo-Barr, D García-Ayu, MP Villegas-P Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration Invest. Ophthalmol. Vis. Sci., 2018;59(11):4392-4403. 2018 [PMID: 30193320] (In Vivo, Rat) In Vivo Rat
KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018;9(6):596. 2018 [PMID: 29789529] (Bioassay, Mouse) Bioassay Mouse
R Rajagopal, S Zhang, X Wei, T Doggett, S Adak, J Enright, V Shah, G Ling, S Chen, J Yoshino, FF Hsu, CF Semenkovic Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision JCI Insight, 2018;3(1):. 2018 [PMID: 29321376] (Bioassay, Rat) Bioassay Rat
SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins Front Pharmacol, 2017;8(0):73. 2017 [PMID: 28280466] (Bioassay, Mouse) Bioassay Mouse
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Bioassay, Mouse) Bioassay Mouse
CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 2016;57(10):4017-26. 2016 [PMID: 27494343] (Bioassay, Human) Bioassay Human
A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016;0(0):. 2016 [PMID: 27226316] (Bioassay, Rat) Bioassay Rat
Show All 26 Publications.

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Bioinformatics

Gene Symbol Cntf
Uniprot