Recombinant Rat CNTF Protein


There are currently no images for CNTF (557-NT).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity RtSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Rat CNTF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 3‑15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED50 for this effect is 0.1-0.3 ng/mL.
E. coli-derived rat CNTF protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
22.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
557-NT in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat CNTF Protein

  • ciliary neurotrophic factor
  • CNTF


Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems’ cytokine catalog.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Ca, Eq
Species: Hu
Species: Hu
Applications: WB, Neut
Species: Hu
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Rt
Species: Hu
Applications: WB, Neut
Species: Hu, Mu, Rt
Applications: WB, IP, ICC, ICFlow, KO
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Mu
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
Species: Rt
Applications: Bioactivity

Publications for CNTF (557-NT)(22)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Avian, Chicken, Zebrafish.

We have publications tested in 2 applications: Bioassay, In Vivo.

Filter By Application
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 557-NT Applications Species
KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 2018;9(6):596. 2018 [PMID: 29789529] (Bioassay, Mouse) Bioassay Mouse
R Rajagopal, S Zhang, X Wei, T Doggett, S Adak, J Enright, V Shah, G Ling, S Chen, J Yoshino, FF Hsu, CF Semenkovic Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision JCI Insight, 2018;3(1):. 2018 [PMID: 29321376] (Bioassay, Rat) Bioassay Rat
SP Jenkinson, D Grandgirar, M Heidemann, A Tscherter, MA Avondet, SL Leib Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins Front Pharmacol, 2017;8(0):73. 2017 [PMID: 28280466] (Bioassay, Mouse) Bioassay Mouse
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Bioassay, Mouse) Bioassay Mouse
CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 2016;57(10):4017-26. 2016 [PMID: 27494343] (Bioassay, Human) Bioassay Human
A system to study mechanisms of neuromuscular junction development and maintenance Development, 2016;0(0):. 2016 [PMID: 27226316] (Bioassay, Rat) Bioassay Rat
Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 2015;10(6):e0129944. 2015 [PMID: 26083449] (Bioassay, Mouse) Bioassay Mouse
Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 2014;33(14):1582-98. 2014 [PMID: 24920579] (Bioassay, Mouse) Bioassay Mouse
Elsaeidi, Fairouz, Bemben, Michael, Zhao, Xiao-Fen, Goldman, Daniel Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci, 2014;34(7):2632-44. 2014 [PMID: 24523552] (Bioassay, Zebrafish) Bioassay Zebrafish
Severi I, Perugini J, Mondini E, Smorlesi A, Frontini A, Cinti S, Giordano A Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 2013;7(0):263. 2013 [PMID: 24409114] (In Vivo, Mouse) In Vivo Mouse
Show All 22 Publications.

Reviews for CNTF (557-NT) (0)

There are no reviews for CNTF (557-NT). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CNTF (557-NT) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

GFAP Antibody
IL-6 [Unconjugated]

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Rat CNTF Protein and receive a gift card or discount.


Gene Symbol Cntf