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VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND50 is typ-ically 0.3-2.4 µg/mL." class="big_lightbox" target="_blank">
557-NT) was serially diluted and captured by Mouse Anti-Rat CNTF Monoclonal Antibody (Catalog # MAB557) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND50 is typically 7‑15 µg/mL." class="big_lightbox" target="_blank">
257-NT) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human CNTF (400 ng/mL) is neutralized (green line) by increasing concentrations of Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA). The ND50 is typically 3-8 µg/mL." class="big_lightbox" target="_blank">
257-NT) was serially diluted and captured by Mouse Anti-Human CNTF Monoclonal Antibody (Catalog # MAB657) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-257-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. FUNCTION: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Nervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. SIMILARITY: Belongs to the CNTF family.
![Immunohistochemistry CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AF557NA_Immunohistochemistry_22749.jpg)
![Immunohistochemistry CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AB-557-NA_Immunohistochemistry_7098.jpg)
![Neutralization CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AF-257-NA_Block_Neutralize_640.jpg)
![N/A CNTF [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CNTF_DNT00_ELISA_779.jpg)
![N/A CNTF [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CNTF_DY257_ELISA_1848.jpg)
![Bioactivity CNTF [Unconjugated]](http://images.novusbio.com/fullsize/protein/CNTF_257-NT_520.jpg)
![Immunohistochemistry CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AF557NA_Immunohistochemistry_22748.jpg)
![Immunohistochemistry CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AF557NA_Immunohistochemistry_23058.jpg)
![Neutralization CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AF-557-NA_Block_Neutralize_8738.jpg)
![ELISA CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/af-557-na_mouse-rat-cntf-affinity-purified-polyclonal-ab-elisa-639201854763058932.jpg)
![Neutralization CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/CNTF_AB-557-NA_Block_Neutralize_8634.jpg)
![ELISA CNTF Antibody [Unconjugated]](http://images.novusbio.com/images/af-257-na_human-cntf-affinity-purified-polyclonal-ab-elisa-639201854763068489.jpg)
![N/A CNTF [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_CNTF_DNT00_ELISA_780.jpg)
![N/A CNTF [HRP]](http://images.novusbio.com/fullsize/elisa/CNTF_DNT00_ELISA_153.jpg)
![SDS-Page CNTF [Unconjugated]](http://images.novusbio.com/fullsize/protein/CNTF_257-NT_502.jpg)
