Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse VCAM-1/CD106 protein
Met Mouse VCAM-1
(Phe25-Glu698)
Accession # CAA47989
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Vcam1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
101.9 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
120 kDa, reducing conditions
Publications
Read Publications using
643-VM in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7. The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity.

During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation.

ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

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Publications for VCAM-1/CD106 (643-VM)(39)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, Flow Cytometry.


Filter By Application
Bioassay
(35)
Flow Cytometry
(2)
All Applications
Filter By Species
Human
(6)
Mouse
(31)
Rat
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using 643-VM Applications Species
MC Schmid, SQ Khan, MM Kaneda, P Pathria, R Shepard, TL Louis, S Anand, G Woo, C Leem, MH Faridi, T Geraghty, A Rajagopala, S Gupta, M Ahmed, RI Vazquez-Pa, DA Cheresh, V Gupta, JA Varner Integrin CD11b activation drives anti-tumor innate immunity Nat Commun, 2018;9(1):5379. 2018 [PMID: 30568188] (Bioassay, Mouse) Bioassay Mouse
J Chen, N Li, Y Yin, N Zheng, M Min, B Lin, L Zhang, X Long, Y Zhang, Z Cai, S Zhai, J Qin, X Wang Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells Cell Rep, 2018;25(12):3393-3404.e6. 2018 [PMID: 30566865] (Bioassay, Mouse) Bioassay Mouse
V Andreani, S Ramamoorth, A Pandey, E Lupar, SL Nutt, T Lämmermann, R Grosschedl Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and ?1-integrin function Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30257949] (Bioassay, Mouse) Bioassay Mouse
FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018;7(0):. 2018 [PMID: 30187863] (Mouse) Mouse
A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis Sci Rep, 2018;8(1):7473. 2018 [PMID: 29748664] (Bioassay, Mouse) Bioassay Mouse
K Morita, C Tokushige, S Maeda, H Kiyose, M Noura, A Iwai, M Yamada, G Kashiwazak, J Taniguchi, T Bando, M Hirata, TR Kataoka, T Nakahata, S Adachi, H Sugiyama, Y Kamikubo RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice Blood Adv, 2018;2(5):509-515. 2018 [PMID: 29500219] (Bioassay, Mouse) Bioassay Mouse
M Praetner, G Zuchtriege, M Holzer, B Uhl, J Schaubäche, L Mittmann, M Fabritius, R Fürst, S Zahler, D Funken, M Lerchenber, A Khandoga, S Kanse, K Lauber, F Krombach, CA Reichel Plasminogen Activator Inhibitor-1 Promotes Neutrophil Infiltration and Tissue Injury on Ischemia-Reperfusion Arterioscler. Thromb. Vasc. Biol., 2018;0(0):. 2018 [PMID: 29371242] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Paetau, T Rolova, L Ning, CG Gahmberg Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia Front Mol Neurosci, 2017;10(0):431. 2017 [PMID: 29311819] (Bioassay, Mouse) Bioassay Mouse
CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex Cell. Signal., 2017;40(0):62-72. 2017 [PMID: 28851628] (Bioassay, Mouse) Bioassay Mouse
PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28637896] (Bioassay, Mouse) Bioassay Mouse
Show All 39 Publications.

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Bioinformatics

Gene Symbol Vcam1
Uniprot