Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10 4 cells/well are added to mouse VCAM-1 coated plates (10 µg/mL with 100 µL/well), >85% will adhere after 1 hour incubation at room temperature. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived mouse VCAM-1/CD106 protein

  Met

  Mouse VCAM-1 (Phe25-Glu698) Accession # CAA47989

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  6-His tag

  N-terminus

  C-terminus

• Accession #: CAA47989
• N-terminal Sequence: Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Vcam1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 101.9 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 120 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse VCAM-1/CD106 Fc Chimera Protein, CF

• CD106 antigen
• CD106
• DKFZp779G2333
• INCAM-100
• L1CAM
• MGC99561
• vascular cell adhesion molecule 1
• vascular cell adhesion protein 1
• V-CAM 1
• VCAM1
• VCAM-1

Background

VCAM-1 (CD106), a member of the immunoglobulin superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta , IL-4, TNF-alpha and IFN-gamma . VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7 . The human and mouse VCAM-1 proteins share approximately 76% amino acid similarity. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Publications for VCAM-1/CD106 (643-VM)(53)

Publications using 643-VM

• Jones, RD;Salim, K;Stankiewicz, LN;Edgar, JM;Leon, L;Gillies, JK;Murtaza, A;Durland, LJ;Raval, D;Lau, C;Taberna, TPB;Hsu, HH;Zimmerman, C;Michaels, YS;Rossi, FMV;Levings, MK;Zandstra, PW; Tunable differentiation of human CD4+ and CD8+ T cells from pluripotent stem cells Cell stem cell 2026-01-08 [PMID: 41512836] (Bioassay, Human)
• Pareja, J;Aydin, S;Zbinden, M;Bouillet, E;Zollinger, N;Theivendram, V;Fahmi, A;Pleska?, P;Barcos, S;Paas, F;Kloster, F;Blázquez, AM;Fonta, N;Merkler, D;Deutsch, U;Engelhardt, B; Lack of junctional adhesion molecule (JAM)-B traps CD8 T cells in CNS border zones and ameliorates autoimmune neuroinflammation Acta neuropathologica communications 2025-05-26 [PMID: 40420242] (Bioassay, Mouse)
• Angiari, S;Carlucci, T;Budui, SL;Bach, SD;Dusi, S;Walter, J;Ellmeier, E;Schnabl, A;Stracke, A;Bordag, N;Tafrali, C;Demjaha, R;Khalil, M;Angelini, G;Terrabuio, E;Pietronigro, EC;Zenaro, E;Laudanna, C;Rossi, B;Constantin, G; Coenzyme A fueling with pantethine limits autoreactive T cell pathogenicity in experimental neuroinflammation Journal of neuroinflammation 2024-11-05 [PMID: 39501296] (Bioassay, Mouse)
• Haage, TR;Charakopoulos, E;Bhuria, V;Baldauf, CK;Korthals, M;Handschuh, J;Müller, P;Li, J;Harit, K;Nishanth, G;Frey, S;Böttcher, M;Fischer, KD;Dudeck, J;Dudeck, A;Lipka, DB;Schraven, B;Green, AR;Müller, AJ;Mougiakakos, D;Fischer, T; Neutrophil-specific expression of JAK2-V617F or CALRmut induces distinct inflammatory profiles in myeloproliferative neoplasia Journal of hematology & oncology 2024-06-09 [PMID: 38853260] (Bioassay, Mouse)
• Juilland, M;Alouche, N;Ubezzi, I;Gonzalez, M;Rashid, HO;Scarpellino, L;Erdmann, T;Grau, M;Lenz, G;Luther, SA;Thome, M; Identification of Tensin-3 as a MALT1 substrate that controls B cell adhesion and lymphoma dissemination Proceedings of the National Academy of Sciences of the United States of America 2023-12-26 [PMID: 38109544] (Bioassay, Mouse)
• Park, JS;Kim, JH;Soh, WC;Kim, NY;Lee, KS;Kim, CH;Chung, IJ;Lee, S;Kim, HR;Jun, CD; Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion Nature communications 2023-05-24 [PMID: 37221214] (Bioassay, Mouse)
• YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Cell Culture, Human)
• S Sevilla-Mo, P Fuentes, Y Rodríguez-, N Arellano-S, PW Krenn, SI de Val, S Montero-He, J García-Cec, V Burdiel-He, SR Gardeta, N Aguilera-M, C Barrio-Alo, G Crainiciuc, D Bouvard, A García-Par, AG Zapata, A Hidalgo, R Fässler, YR Carrasco, ML Toribio, J Teixidó ICAP-1 loss impairs CD8+ thymocyte development and leads to reduced marginal zone B cells in mice European Journal of Immunology, 2022-05-13;0(0):. 2022-05-13 [PMID: 35491946] (Bioassay, Mouse)
• CM Lee, ML Chang, RH Chen, FW Chen, JC Liu, SL Kuo, HH Peng Thrombin-Activated Platelets Protect Vascular Endothelium against Tumor Cell Extravasation by Targeting Endothelial VCAM-1 International Journal of Molecular Sciences, 2022-03-22;23(7):. 2022-03-22 [PMID: 35408794] (Cell Culture, Mouse)
• Z Benet, Z Jing, DR Fooksman Plasma cell dynamics in the bone marrow niche Cell Reports, 2021-02-09;34(6):108733. 2021-02-09 [PMID: 33567286] (Bioassay, Mouse)
• NH Roy, SHJ Kim, A Buffone, D Blumenthal, B Huang, S Agarwal, PL Schwartzbe, DA Hammer, JK Burkhardt LFA-1 signals to promote actin polymerization and upstream migration in T cells J. Cell. Sci., 2020-09-09;133(17):. 2020-09-09 [PMID: 32907931] (Bioassay, Mouse)
• V Azcutia, M Kelm, AC Luissint, K Boerner, S Flemming, M Quiros, G Newton, A Nusrat, FW Luscinskas, CA Parkos Neutrophil expressed CD47 regulates CD11b/CD18-dependent neutrophil transepithelial migration in the intestine in vivo Mucosal Immunol, 2020-06-19;0(0):. 2020-06-19 [PMID: 32561828] (Bioassay, Mouse)
• V Barbier, J Erbani, C Fiveash, JM Davies, J Tay, MR Tallack, J Lowe, JL Magnani, DR Pattabiram, AC Perkins, J Lisle, JEJ Rasko, JP Levesque, IG Winkler Endothelial E-selectin inhibition improves acute myeloid leukaemia therapy by disrupting vascular niche-mediated chemoresistance Nat Commun, 2020-04-27;11(1):2042. 2020-04-27 [PMID: 32341362] (Bioassay, Mouse)
• K Birkner, B Wasser, T Ruck, C Thalman, D Luchtman, K Pape, S Schmaul, L Bitar, EM Krämer-Alb, A Stroh, SG Meuth, F Zipp, S Bittner beta1-integrin- and KV1.3 channel-dependent signaling stimulates glutamate release from Th17 cells J. Clin. Invest., 2020-02-03;0(0):. 2020-02-03 [PMID: 31661467] (Mouse)
• MC Schmid, SQ Khan, MM Kaneda, P Pathria, R Shepard, TL Louis, S Anand, G Woo, C Leem, MH Faridi, T Geraghty, A Rajagopala, S Gupta, M Ahmed, RI Vazquez-Pa, DA Cheresh, V Gupta, JA Varner Integrin CD11b activation drives anti-tumor innate immunity Nat Commun, 2018-12-19;9(1):5379. 2018-12-19 [PMID: 30568188] (Bioassay, Mouse)
• J Chen, N Li, Y Yin, N Zheng, M Min, B Lin, L Zhang, X Long, Y Zhang, Z Cai, S Zhai, J Qin, X Wang Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells Cell Rep, 2018-12-18;25(12):3393-3404.e6. 2018-12-18 [PMID: 30566865] (Bioassay, Mouse)
• V Andreani, S Ramamoorth, A Pandey, E Lupar, SL Nutt, T Lämmermann, R Grosschedl Cochaperone Mzb1 is a key effector of Blimp1 in plasma cell differentiation and ?1-integrin function Proc. Natl. Acad. Sci. U.S.A., 2018-09-26;0(0):. 2018-09-26 [PMID: 30257949] (Bioassay, Mouse)
• FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018-09-06;7(0):. 2018-09-06 [PMID: 30187863] (Mouse)
• A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis Sci Rep, 2018-05-10;8(1):7473. 2018-05-10 [PMID: 29748664] (Bioassay, Mouse)
• K Morita, C Tokushige, S Maeda, H Kiyose, M Noura, A Iwai, M Yamada, G Kashiwazak, J Taniguchi, T Bando, M Hirata, TR Kataoka, T Nakahata, S Adachi, H Sugiyama, Y Kamikubo RUNX transcription factors potentially control E-selectin expression in the bone marrow vascular niche in mice Blood Adv, 2018-03-13;2(5):509-515. 2018-03-13 [PMID: 29500219] (Bioassay, Mouse)
• M Praetner, G Zuchtriege, M Holzer, B Uhl, J Schaubäche, L Mittmann, M Fabritius, R Fürst, S Zahler, D Funken, M Lerchenber, A Khandoga, S Kanse, K Lauber, F Krombach, CA Reichel Plasminogen Activator Inhibitor-1 Promotes Neutrophil Infiltration and Tissue Injury on Ischemia-Reperfusion Arterioscler. Thromb. Vasc. Biol., 2018-01-25;0(0):. 2018-01-25 [PMID: 29371242] (Flow Cytometry, Mouse)
• S Sarvi, H Patel, J Li, GL Dodd, H Creedon, M Muir, J Ward, JC Dawson, M Lee, J Culley, DM Salter, AH Sims, A Byron, VG Brunton Kindlin-1 promotes pulmonary breast cancer metastasis Cancer Res., 2018-01-12;0(0):. 2018-01-12 [PMID: 29330144] (Bioassay, Mouse)
• S Paetau, T Rolova, L Ning, CG Gahmberg Neuronal ICAM-5 Inhibits Microglia Adhesion and Phagocytosis and Promotes an Anti-inflammatory Response in LPS Stimulated Microglia Front Mol Neurosci, 2017-12-22;10(0):431. 2017-12-22 [PMID: 29311819] (Bioassay, Mouse)
• CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex Cell. Signal., 2017-08-26;40(0):62-72. 2017-08-26 [PMID: 28851628] (Bioassay, Mouse)
• PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017-06-21;0(0):. 2017-06-21 [PMID: 28637896] (Bioassay, Mouse)
• KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity Nat. Immunol., 2017-04-17;0(0):. 2017-04-17 [PMID: 28414311] (Bioassay, Mouse)
• S Shukla, MA Langley, J Singh, JM Edgar, M Mohtashami, JC Zúñiga-Pfl, PW Zandstra Progenitor T-cell differentiation from hematopoietic stem cells using Delta-like-4 and VCAM-1 Nat. Methods, 2017-04-10;14(5):531-538. 2017-04-10 [PMID: 28394335] (Bioassay, Mouse)
• ML Estin, SB Thompson, B Traxinger, MH Fisher, RS Friedman, J Jacobelli Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration Proc. Natl. Acad. Sci. U.S.A, 2017-03-20;0(0):. 2017-03-20 [PMID: 28320969] (Bioassay, Mouse)
• Y Hirano, WL Yang, M Aziz, F Zhang, B Sherry, P Wang MFG-E8-derived peptide attenuates adhesion and migration of immune cells to endothelial cells J. Leukoc. Biol, 2017-01-17;0(0):. 2017-01-17 [PMID: 28096298] (Bioassay, Mouse)
• Beta 1-integrin ligation and TLR ligation enhance GM-CSF-induced ALDH1A2 expression in dendritic cells, but differentially regulate their anti-inflammatory properties Sci Rep, 2016-11-29;6(0):37914. 2016-11-29 [PMID: 27897208] (Bioassay, Mouse)
• Aged neutrophils contribute to the first line of defense in the acute inflammatory response Blood, 2016-09-08;0(0):. 2016-09-08 [PMID: 27609642] (Flow Cytometry, Mouse)
• A Critical Role for P2X7 Receptor-Induced VCAM-1 Shedding and Neutrophil Infiltration during Acute Lung Injury J Immunol, 2016-08-24;0(0):. 2016-08-24 [PMID: 27559050] (Bioassay, Rat)
• Henriette Rudolph Post-arrest stalling rather than crawling favors CD8(+) over CD4(+) T-cell migration across the blood-brain barrier under flow in vitro Eur J Immunol, 2016-07-22;0(0):. 2016-07-22 [PMID: 27338806] (Bioassay, Mouse)
• Platelets Guide Leukocytes to Their Sites of Extravasation PLoS Biol, 2016-05-06;14(5):e1002459. 2016-05-06 [PMID: 27152726] (Bioassay, Mouse)
• Martin-Blondel G, Pignolet B, Tietz S, Yshii L, Gebauer C, Perinat T, Van Weddingen I, Blatti C, Engelhardt B, Liblau R Migration of encephalitogenic CD8 T cells into the central nervous system is dependent on the alpha4beta1-integrin. Eur J Immunol, 2015-10-06;45(12):3302-12. 2015-10-06 [PMID: 26358409] (Bioassay, Mouse)
• Lemaire M, Negro Silva L, Lemarie C, Bolt A, Flores Molina M, Krohn R, Smits J, Lehoux S, Mann K Arsenic Exposure Increases Monocyte Adhesion to the Vascular Endothelium, a Pro-Atherogenic Mechanism. PLoS ONE, 2015-09-02;10(9):e0136592. 2015-09-02 [PMID: 26332580] (Bioassay, Human, Mouse)
• Boularan C, Hwang I, Kamenyeva O, Park C, Harrison K, Huang Z, Kehrl J B Lymphocyte-Specific Loss of Ric-8A Results in a Galpha Protein Deficit and Severe Humoral Immunodeficiency. J Immunol, 2015-07-31;195(5):2090-102. 2015-07-31 [PMID: 26232433] (Bioassay, Mouse)
• Totsuka N, Kim Y, Kanemaru K, Niizuma K, Umemoto E, Nagai K, Tahara-Hanaoka S, Nakahasi-Oda C, Honda S, Miyasaka M, Shibuya K, Shibuya A Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration. Nat Commun, 2014-08-19;5(0):4710. 2014-08-19 [PMID: 25134989] (Bioassay, Mouse)
• Palmi C, Fazio G, Savino A, Procter J, Howell L, Cazzaniga V, Vieri M, Longinotti G, Brunati I, Andre V, Della Mina P, Villa A, Greaves M, Biondi A, D'Amico G, Ford A, Cazzaniga G Cytoskeletal regulatory gene expression and migratory properties of B-cell progenitors are affected by the ETV6-RUNX1 rearrangement. Mol Cancer Res, 2014-07-24;12(12):1796-806. 2014-07-24 [PMID: 25061103] (Bioassay, Mouse)
• Gobel K, Schuhmann M, Pankratz S, Stegner D, Herrmann A, Braun A, Breuer J, Bittner S, Ruck T, Wiendl H, Kleinschnitz C, Nieswandt B, Meuth S Phospholipase D1 mediates lymphocyte adhesion and migration in experimental autoimmune encephalomyelitis. Eur J Immunol, 2014-06-05;44(8):2295-305. 2014-06-05 [PMID: 24811005] (Bioassay, Mouse)
• Wang X, Rodda L, Bannard O, Cyster J Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. J Immunol, 2014-04-16;192(10):4601-9. 2014-04-16 [PMID: 24740506] (Bioassay, Mouse)
• Gonzalvo-Feo S, Del Prete A, Pruenster M, Salvi V, Wang L, Sironi M, Bierschenk S, Sperandio M, Vecchi A, Sozzani S Endothelial cell-derived chemerin promotes dendritic cell transmigration. J Immunol, 2014-01-27;192(5):2366-73. 2014-01-27 [PMID: 24470498] (Bioassay, Mouse)
• Chen Y, Molnar M, Li L, Friberg P, Gan L, Brismar H, Fu Y Characterization of VCAM-1-binding peptide-functionalized quantum dots for molecular imaging of inflamed endothelium. PLoS ONE, 2013-12-31;8(12):e83805. 2013-12-31 [PMID: 24391829] (Bioassay, Mouse)
• Sato S, Sakurai T, Ogasawara J, Takahashi M, Izawa T, Imaizumi K, Taniguchi N, Ohno H, Kizaki T A circadian clock gene, Rev-erbalpha, modulates the inflammatory function of macrophages through the negative regulation of Ccl2 expression. J Immunol, 2013-12-04;192(1):407-17. 2013-12-04 [PMID: 24307731] (Bioassay, Mouse)
• Lee J, Sohn J, Ryu S, Hong C, Moon K, Park J A novel human anti-VCAM-1 monoclonal antibody ameliorates airway inflammation and remodelling. J Cell Mol Med, 2013-07-16;17(10):1271-81. 2013-07-16 [PMID: 23855490] (Bioassay, Human)
• Schmid M, Franco I, Kang S, Hirsch E, Quilliam L, Varner J PI3-kinase gamma promotes Rap1a-mediated activation of myeloid cell integrin alpha4beta1, leading to tumor inflammation and growth. PLoS ONE, 2013-04-02;8(4):e60226. 2013-04-02 [PMID: 23565202] (Bioassay, Mouse)
• Harker K, Ueno N, Wang T, Bonhomme C, Liu W, Lodoen M Toxoplasma gondii modulates the dynamics of human monocyte adhesion to vascular endothelium under fluidic shear stress. J Leukoc Biol, 2013-03-13;93(5):789-800. 2013-03-13 [PMID: 23485448] (Bioassay, Human)
• Alcaide P, Maganto-Garcia E, Newton G, Travers R, Croce KJ, Bu DX, Luscinskas FW, Lichtman AH Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J. Immunol., 2012-01-04;188(3):1421-30. 2012-01-04 [PMID: 22219321] (Bioassay, Mouse)
• Moser M, Bauer M, Schmid S, Ruppert R, Schmidt S, Sixt M, Wang HV, Sperandio M, Fassler R Kindlin-3 is required for beta2 integrin-mediated leukocyte adhesion to endothelial cells. Nat. Med., 2009-02-22;15(3):300-5. 2009-02-22 [PMID: 19234461] (Bioassay, Mouse)
• Sasaki K, Pardee AD, Okada H, Storkus WJ IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit. Eur. J. Immunol., 2008-10-01;38(10):2865-73. 2008-10-01 [PMID: 18958887] (Bioassay, Mouse)
• Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S High pressure promotes monocyte adhesion to the vascular wall. Circ. Res., 2007-03-29;100(8):1226-33. 2007-03-29 [PMID: 17395876] (Bioassay, Human)
• Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J. Leukoc. Biol., 2006-12-18;81(3):748-56. 2006-12-18 [PMID: 17178915] (Bioassay, Human, Mouse)
• Cardones AR, Murakami T, Hwang ST CXCR4 enhances adhesion of B16 tumor cells to endothelial cells in vitro and in vivo via beta(1) integrin. Cancer Res., 2003-10-15;63(20):6751-7. 2003-10-15 [PMID: 14583470] (Bioassay, Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Vcam1
• Uniprot:
  • CAA47989()