Recombinant Mouse LIGHT/TNFSF14 Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse LIGHT/TNFSF14 Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using HT‑29 human colon adenocarcinoma cells Yu, K.Y. et al. (1999) J. Biol. Chem. 274:13733; Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548; Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142. The ED50 for this effect is 2-5 µg/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) and recombinant human IFN-gamma.
Source
Mouse myeloma cell line, NS0-derived mouse LIGHT/TNFSF14 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR
Mouse LIGHT
(Asp72-Val239)
Accession # Q9QYH9
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
Tnfsf14
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
24-34 kDa, reducing conditions
Publications
Read Publications using
1794-LT in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse LIGHT/TNFSF14 Protein

  • CD258 antigen
  • CD258
  • delta transmembrane LIGHT
  • Herpes virus entry mediator ligand
  • Herpesvirus entry mediator ligand
  • HVEM-L
  • HVEMLherpesvirus entry mediator-ligand
  • ligand for herpesvirus entry mediator
  • LIGHT
  • LIGHTherpesvirus entry mediator A
  • LTg
  • TNFSF14
  • TR2
  • tumor necrosis factor (ligand) superfamily, member 14
  • tumor necrosis factor ligand superfamily member 14
  • tumor necrosis factor receptor-like 2
  • tumor necrosis factor superfamily member LIGHT

Background

LIGHT (lymphotoxin-like, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes) is a member of the TNF superfamily and is designated TNFSF14. The gene for mouse LIGHT encodes a 239 amino acid residue (aa) type II transmembrane glycoprotein that contains a 37 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 181 aa extracellular domain. A soluble form of mouse LIGHT is generated from the membrane form by proteolytic processing. Similar to other TNF ligand family members, LIGHT is assembled as a homotrimer. Mouse and human LIGHT share 71% aa sequence identity.

LIGHT is expressed by activated lymphocytes, natural killer cells, immature dendritic cells, monocytes and granulocytes. Mouse LIGHT binds and signals via two distinct TNF receptor superfamily members, including the herpes virus entry mediator (HVEM/TNFRSF14) and the lymphotoxin beta receptor (LT beta R/TNFRSF3). In humans, LIGHT also binds the soluble human decoy receptor 3 (DcR3/TNFRSF6B). Signaling from LT beta R, which also binds LT alpha beta, induces apoptosis and the production of various cytokines. LIGHT-LT beta R signaling also plays a role in mesenteric lymph node organogenesis, and restoration of secondary lymphoid structure and function. Signaling from HVEM, which also binds LT alpha, co-stimulates T-helper cell type 1 (TH1) immune responses, enhances Cytotoxic T Lymphocytes (CTL)‑mediated tumor immunity, and regulates allogeneic T cell activation and allograft rejection. Blockade of LIGHT-HVEM signaling has been shown to prevent graft versus host disease.

  1. Granger, S.W. and S. Rickert (2003) Cytokine Growth Factor Rev. 14:289.

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1794-LT
Species: Mu
Applications: Bioactivity

Publications for LIGHT/TNFSF14 (1794-LT)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(4)
In Vivo
(3)
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Mouse
(7)
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Showing Publications 1 - 7 of 7.
Publications using 1794-LT Applications Species
A TNFRSF14-Fc?RI-mast cell pathway contributes to development of multiple features of asthma pathology in mice Nat Commun, 2016;7(0):13696. 2016 [PMID: 27982078] (Bioassay, Mouse) Bioassay Mouse
Herro R, Antunes R, Aguilera A, Tamada K, Croft M The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis. J Invest Dermatol, 2015;135(8):2109-18. 2015 [PMID: 25789702] (In Vivo, Mouse) In Vivo Mouse
Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012;109(25):9971-6. 2012 [PMID: 22665806] (Bioassay, Mouse) Bioassay Mouse
Doherty TA, Soroosh P, Khorram N, Fukuyama S, Rosenthal P, Cho JY, Norris PS, Choi H, Scheu S, Pfeffer K, Zuraw BL, Ware CF, Broide DH, Croft M The tumor necrosis factor family member LIGHT is a target for asthmatic airway remodeling. Nat. Med., 2011;17(5):596-603. 2011 [PMID: 21499267] (In Vivo, Mouse) In Vivo Mouse
Aebischer J, Cassina P, Otsmane B, Moumen A, Seilhean D, Meininger V, Barbeito L, Pettmann B, Raoul C FN-gamma triggers a LIGHT-dependent selective death of motoneurons contributing to the non-cell-autonomous effects of mutant SOD1. Cell Death Differ., 2011;18(5):754-68. 2011 [PMID: 21072055] (Bioassay, Mouse) Bioassay Mouse
Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009;384(3):296-300. 2009 [PMID: 19406102] (In Vivo, Mouse) In Vivo Mouse
Mueller AM, Pedre X, Killian S, David M, Steinbrecher A The Decoy Receptor 3 (DcR3, TNFRSF6B) suppresses Th17 immune responses and is abundant in human cerebrospinal fluid. J. Neuroimmunol., 2009;209(1):57-64. 2009 [PMID: 19269042] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol Tnfsf14
Uniprot