LIGHT/TNFSF14 Antibodies

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="LIGHT/TNFSF14 was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human LIGHT/ TNFSF14 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LIGHT/ TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664). The ND₅₀ is typically 5-20 ng/mL." class="big_lightbox"> 664-LI) induces proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human LIGHT/ TNFSF14 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human LIGHT/ TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664). The ND₅₀ is typically 5-20 ng/mL." class="big_thumb" />

MAB6642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> MAB6642) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

664-LI) was serially diluted and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 664-LI) was serially diluted and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="PBMC treated with 50ng/mL PMA and 500ng/mL Calcium Ionomycin for 48 hours (A) vs resting PBMC (B) were stained with Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) and Mouse Anti-Human CD8 alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB1509P) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

664-LI) was serially diluted and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 664-LI) was serially diluted and captured by Mouse Anti-Human LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB664) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIGHT/TNFSF14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF664) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with either (A) mouse LIGHT/TNFSF14 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse LIGHT/TNFSF14 Monoclonal Antibody (Catalog # MAB17942) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />