Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). ...read more
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 ...read more
Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a ...read more
1 ug/mL of this antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Rhodopsin antibody validated for IHC-Fr from a verified customer review.
40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-59690 in the following applications:
Extinct Spiny Shark (Acanthodes bridgei). Zebrafish reactivity reported from a verified customer review.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4), 50% Glycerol
0.09% Sodium Azide
Protein G purified
Alternate Names for Rhodopsin Antibody (4D2)
opsin 2, rod pigment
retinitis pigmentosa 4, autosomal dominant
Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Put slides in 0.1% PBS-Tween 20 1-2 minutes In 10mM sodium citrate pH 8.5 autoclave (1 min at 121°C, door open at 99⁰C ) Put back in 0.1% PBS-Tween20 Wash 3 x 5min 0.1% PBS-Tween20 1 hour block solution (10% NFDM in 0.1% PBS-Tween20) Overnight with Primary antibody 1:500- 1:5000 in block solution Wash 3 x 10 min 0.1% PBS-Tween20 2 hour incubation with Secondary antibody (1:500) + DAPI (1:10000) in block solution Wash 3 X 10 min 0.1% PBS-Tween20 Enclose with prolong gold antifade Store in dark at 4°C. ***Novus Innovators Program - new species or application used on a primary antibody.
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Vision Infographic: Do you see how I see? Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological... Read full blog post.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.