Rhodopsin Antibody (4D2)

Images

 
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of ~38.9kDa Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). ...read more
Immunohistochemistry: Rhodopsin Antibody (4D2) [NBP2-59690] - Immunohistochemistry analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (NBP2-59690). Tissue: retina. Species: Mouse. Primary Antibody: Mouse ...read more
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Bovine photoreceptor membranes showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 ...read more
Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a ...read more

Product Details

Summary
Reactivity Am, Av, Fi, Ma, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
Clone
4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Rhodopsin Antibody (4D2) Summary

Immunogen
Bovine Rhodopsin
Localization
Membrane
Specificity
Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
1 ug/mL of this antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Rhodopsin antibody validated for IHC-Fr from a verified customer review.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-59690 in the following applications:

Publications
Read Publications using
NBP2-59690 in the following applications:

Reactivity Notes

Extinct Spiny Shark (Acanthodes bridgei). Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rhodopsin Antibody (4D2)

  • CSNBAD1
  • MGC138309
  • OPN2
  • OPN2MGC138311
  • opsin 2, rod pigment
  • Opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • RHO
  • Rhodopsin
  • RP4

Background

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

664-LI/CF
Species: Hu
Applications: BA
NB100-91273
Species: Hu, Mu, Pm, Rt
Applications: ICC/IF, IHC, IHC-P, WB
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
NBP2-67895
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IP, WB
NB100-624
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
NB100-91266
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB200-540
Species: Ec, Mu
Applications: CyTOF-ready, Flow-IC, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
NB120-2776
Species: Bv, Hu
Applications: ICC/IF, IHC, IHC-Fr, IP, WB
NLS1017
Species: Bt, Bv, Ca, Eq, Hu, Mu
Applications: IHC, IHC-P
MAB194
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, WB
NLS418
Species: Hu
Applications: IHC, IHC-P
NLS2756
Species: Hu, Pm
Applications: ICC, IHC, IHC-P
NLS2576
Species: Hu, Pm, Rt
Applications: IHC, IHC-P

Publications for Rhodopsin Antibody (NBP2-59690)(3)

Review for Rhodopsin Antibody (NBP2-59690) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Zebrafish.

Reviews using NBP2-59690:
Filter by Applications
Immunohistochemistry-Frozen
(1)
All Applications
Filter by Species
Zebrafish
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Rhodopsin NBP2-59690
Enlarge
5
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Zebrafish 08/29/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRetina (outer nuclear layer)
SpeciesZebrafish
LotMR187076

Comments

Commentso/n PFA and Methanol fixed 7um sections

Put slides in 0.1% PBS-Tween 20 1-2 minutes
In 10mM sodium citrate pH 8.5 autoclave (1 min at 121°C, door open at 99⁰C )
Put back in 0.1% PBS-Tween20
Wash 3 x 5min 0.1% PBS-Tween20
1 hour block solution (10% NFDM in 0.1% PBS-Tween20)
Overnight with Primary antibody 1:500- 1:5000 in block solution
Wash 3 x 10 min 0.1% PBS-Tween20
2 hour incubation with Secondary antibody (1:500) + DAPI (1:10000) in block solution
Wash 3 X 10 min 0.1% PBS-Tween20
Enclose with prolong gold antifade
Store in dark at 4°C.
***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP2-59690) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Rhodopsin Products

Bioinformatics Tool for Rhodopsin Antibody (NBP2-59690)

Discover related pathways, diseases and genes to Rhodopsin Antibody (NBP2-59690). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Rhodopsin Antibody (NBP2-59690)

Discover more about diseases related to Rhodopsin Antibody (NBP2-59690).
 

Pathways for Rhodopsin Antibody (NBP2-59690)

View related products by pathway.

PTMs for Rhodopsin Antibody (NBP2-59690)

Learn more about PTMs related to Rhodopsin Antibody (NBP2-59690).
 

Research Areas for Rhodopsin Antibody (NBP2-59690)

Find related products by research area.

Blogs on Rhodopsin.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
08/29/2019
Application: Immunohistochemistry-Frozen
Species: Zebrafish

Bioinformatics

Gene Symbol RHO