Rhodopsin Antibody (4D2)

Images

 
Western Blot: Rhodopsin Antibody (4D2) [NBP2-59690] - Western Blot analysis of Human A549 cells showing detection of Rhodopsin protein using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2 (SMC-176). Load: 15 ug. ...read more
Immunocytochemistry/ Immunofluorescence: Rhodopsin Antibody (4D2) [NBP2-59690] - Analysis using Mouse Anti-Rhodopsin Monoclonal Antibody, Clone 4D2. Tissue: retina. Species: Mouse. Primary Antibody: Mouse Anti-Rhodopsin ...read more
Immunohistochemistry-Frozen: Rhodopsin Antibody (4D2) [NBP2-59690] - Zebrafish retina (outer nuclear layer). PFA and methanol fixed 7 um sections. Rhodopsin antibody O/N incubation at 1:500. IHC-Fr image submitted by a ...read more

Product Details

Summary
Reactivity Am, Av, Fi, Ma, ZeSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IP
Clone
4D2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Rhodopsin Antibody (4D2) Summary

Immunogen
Bovine Rhodopsin
Localization
Membrane
Specificity
Binds specifically to the N-terminus of Rhodopsin. Does not detect Rhodopsin in invertebrates.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RHO
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
Application Notes
1 ug/mL of this antibody was sufficient for detection of rhodopsin in 10 ug of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Rhodopsin antibody validated for IHC-Fr from a verified customer review.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-59690.

Reactivity Notes

Extinct Spiny Shark (Acanthodes bridgei). Zebrafish reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Rhodopsin Antibody (4D2)

  • CSNBAD1
  • MGC138309
  • OPN2MGC138311
  • opsin 2, rod pigment
  • opsin-2
  • retinitis pigmentosa 4, autosomal dominant
  • rhodopsin
  • RP4

Background

Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4).Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rhodopsin Antibody (NBP2-59690)(1)

We have publications tested in 1 application: ELISA.


Filter By Application
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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rhodopsin Antibody (NBP2-59690) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-59690AF405
Alexa Fluor 488 NBP2-59690AF488
Alexa Fluor 647 NBP2-59690AF647
Alexa Fluor 700 NBP2-59690AF700
Biotin NBP2-59690B
DyLight 350 NBP2-59690UV
DyLight 405 NBP2-59690V
DyLight 488 NBP2-59690G
DyLight 550 NBP2-59690R
DyLight 650 NBP2-59690C
DyLight 680 NBP2-59690FR
DyLight 755 NBP2-59690IR
FITC NBP2-59690F
HRP NBP2-59690H
Janelia Fluor 549 NBP2-59690JF549
Janelia Fluor 646 NBP2-59690JF646

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Bioinformatics Tool for Rhodopsin Antibody (NBP2-59690)

Discover related pathways, diseases and genes to Rhodopsin Antibody (NBP2-59690). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on Rhodopsin.

Vision Infographic: Do you see how I see?
Vision involves several parts of the eye processing light which send signals to the brain via the optic nerve to process information. Learn more about the vision process and related ocular proteins in the infographic below. Novus Biological...  Read full blog post.

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Bioinformatics

Gene Symbol RHO