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AKR1C2 Antibody (CPTC-AKR1C2-1)

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Western Blot: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Western Blot Analysis of Human HeLa, K-562 and A431 cell lysates using AKR1C2 Antibody (CPTC-AKR1C2-1).
Western Blot: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Western Blot Analysis of Human HeLa, K-562 and A431 cell lysates using AKR1C2 Antibody (CPTC-AKR1C2-1).
Immunohistochemistry-Paraffin: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Antibody (CPTC-AKR1C2-1).
Immunohistochemistry-Paraffin: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Antibody (CPTC-AKR1C2-1).
Protein Array: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AKR1C2 Antibody (CPTC-AKR1C2-1). Z- and S- Score: The Z-score ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PA
Clone
CPTC-AKR1C2-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-79909AF350
Alexa Fluor 405
NBP2-79909AF405
Alexa Fluor 488
NBP2-79909AF488
Alexa Fluor 532
NBP2-79909AF532
Alexa Fluor 594
NBP2-79909AF594
Alexa Fluor 647
NBP2-79909AF647
Alexa Fluor 700
NBP2-79909AF700
Alexa Fluor 750
NBP2-79909AF750
Biotin
NBP2-79909B
DyLight 350
NBP2-79909UV
DyLight 405
NBP2-79909V
DyLight 488
NBP2-79909G
DyLight 550
NBP2-79909R
DyLight 594
NBP2-79909DL594
DyLight 650
NBP2-79909C
DyLight 680
NBP2-79909FR
DyLight 755
NBP2-79909IR
FITC
NBP2-79909F
HRP
NBP2-79909H
Janelia Fluor 549
NBP2-79909JF549
Janelia Fluor 646
NBP2-79909JF646
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-79909
100% Guarantee on every product

AKR1C2 Antibody (CPTC-AKR1C2-1) Summary

Immunogen
Recombinant human full-length AKR1C2 protein
Localization
Cytoplasm
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
AKR1C2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for AKR1C2 Antibody (CPTC-AKR1C2-1)

  • aldo-keto reductase family 1 member C2
  • aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
  • BABP
  • Chlordecone reductase homolog HAKRD
  • DD
  • DD-2
  • DD2DD/BABP
  • DDH2FLJ53800
  • Dihydrodiol dehydrogenase 2
  • Dihydrodiol dehydrogenase/bile acid-binding protein
  • EC 1.1.1,3-alpha-HSD3
  • EC 1.1.1.213
  • EC 1.3.1.20
  • HAKRDAKR1C-pseudo
  • HBAB
  • MCDR2
  • pseudo-chlordecone reductase
  • Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
  • type II dihydrodiol dehydrogenase
  • Type III 3-alpha-hydroxysteroid dehydrogenase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ACE/CD143 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE/CD143 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of mouse lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE/CD143 at approximately 218 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse ACE/CD143 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1513) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.
(1 Review)
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Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
ID1 Antibody
(JM92-13)
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Western Blot: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: ID1 Antibody (JM92-13) [NBP2-66897] - Staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: ID1 Antibody (JM92-13) [NBP2-66897] - Analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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44110002
ELISA: FMN1 Antibody [44110002]
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NB100-1269
Western Blot: FoxC2 Antibody [NB100-1269] - Staining of nuclear HEK293 cell lysate with antibody at 2 ug/mL (A) and negative control human pancreas cell lysate with antibody at 1 ug/mL (B) (RIPA buffer 35 ug total protein per lane). Detected by chemiluminescence.
Flow Cytometry: FoxC2 Antibody [NB100-1269] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Antibody (Ang II E7 ( ...
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Western Blot: Angiotensin II/III Antibody (Ang II E7 (BGN/KA/4L)) [NB100-62346] - Evaluation of angiotensis II expression in untreated UUO rat kidneys and UUO rat kidneys treated with Ang-(1-7).
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LIGHT/TNFSF14
[Unconjugated]
664-LI/CF
1 µg/lane of Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 25 kDa.
Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
(16 Publications)
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Aldo-keto
Reductase 1C1/AKR1C1 ...
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Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and human liver tissue, loaded at 0.5 mg/mL. A specific band was detected for Aldo‑keto Reductase 1C1/AKR1C1 at approximately 41 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Aldo‑keto Reductase 1C1/AKR1C1 Monoclonal Antibody (Catalog # MAB6529). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Hu
Applications: WB, Simple Western
Band 3 Antibody
(BIII 136)
NB120-11012
Western Blot: Band 3 Antibody (BIII 136) [NB120-11012] - Human erythrocytes ghosts extract was separated on SDS-PAGE and probed with Monoclonal Anti-Band 3 Clone: BIII-136. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and AEC substrate.
Species: Hu, Bv(-), Ca(-), Eq(-), Gp(-), Mu(-), Po(-)
Applications: WB, ELISA, ICC/IF, IP
Aldo-keto
Reductase 1C3/AKR1C3
NBP1-31378
Western Blot: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C3 antibody diluted at 1:20000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: Aldo-keto Reductase 1C3/AKR1C3 Antibody [NBP1-31378] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.
Species: Hu, Rt
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II/Thrombin Antibody
NBP1-58268
Western Blot: Coagulation Factor II/Thrombin Antibody [NBP1-58268] - 721_B tissue lysate at a concentration of 1ug/ml.
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[Unconjugated]
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NBP2-94035
Western Blot: TEF1 Antibody [NBP2-94035] - Analysis of extracts of various cell lines, using TEF1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunocytochemistry/Immunofluorescence: TEF1 Antibody [NBP2-94035] - Analysis of extracts of HeLa cell line, using TEF1 rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunohistochemistry-Paraffin: TEF1 Antibody [NBP2-94035] - Paraffin-embedded mouse lung using TEF1.
Species: Hu, Mu
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Immunocytochemistry/Immunofluorescence: t-Plasminogen Activator/tPA Antibody (AH54-10) [NBP2-67279] - Staining TPA in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Antibody
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Western Blot: SLC17A5 Antibody [NBP2-20383] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: SLC17A5 Antibody [NBP2-20383] - Sample: HepG2 cells were fixed in -20C 100% MeOH for 5 min. Green: SLC17A5 protein stained by SLC17A5 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: Sialin/SLC17A5 Antibody [NBP2-20383] - Mouse lung diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Renin was detected in immersion fixed paraffin-embedded sections of human kidney using Sheep Anti-Human Renin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4090) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: AGTR-1 Antibody [NBP1-77078] - Mouse Kidney cells at 20 ug/ml.
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Western Blot: Aldo-keto Reductase 1C4/AKR1C4 Antibody (2C11) [H00001109-M01] - Aldo-keto Reductase 1C4/AKR1C4 monoclonal antibody (M01), clone 2C11 Western Blot analysis of Aldo-keto Reductase 1C4/AKR1C4 expression in MCF-7 [L046V1].
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AKR1C2 Antibody
(CPTC-AKR1C2-1)
NBP2-79775
AKR1C2 Antibody (CPTC-AKR1C2-1)
Species: Hu
Applications: WB, IHC, IHC-P, PA

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-79909AF350
Alexa Fluor 405 NBP2-79909AF405
Alexa Fluor 488 NBP2-79909AF488
Alexa Fluor 532 NBP2-79909AF532
Alexa Fluor 594 NBP2-79909AF594
Alexa Fluor 647 NBP2-79909AF647
Alexa Fluor 700 NBP2-79909AF700
Alexa Fluor 750 NBP2-79909AF750
Biotin NBP2-79909B
DyLight 350 NBP2-79909UV
DyLight 405 NBP2-79909V
DyLight 488 NBP2-79909G
DyLight 550 NBP2-79909R
DyLight 594 NBP2-79909DL594
DyLight 650 NBP2-79909C
DyLight 680 NBP2-79909FR
DyLight 755 NBP2-79909IR
FITC NBP2-79909F
HRP NBP2-79909H
Janelia Fluor 549 NBP2-79909JF549
Janelia Fluor 646 NBP2-79909JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol AKR1C2
Uniprot