AKR1C2 Antibody (CPTC-AKR1C2-1)

Western Blot: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Western Blot Analysis of Human HeLa, K-562 and A431 cell lysates using AKR1C2 Antibody (CPTC-AKR1C2-1).

Immunohistochemistry-Paraffin: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Antibody (CPTC-AKR1C2-1).

Immunohistochemistry-Paraffin: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AKR1C2 Antibody (CPTC-AKR1C2-1).

Protein Array: AKR1C2 Antibody (CPTC-AKR1C2-1) [NBP2-79775] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AKR1C2 Antibody (CPTC-AKR1C2-1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: WB, IHC, MA
• Clone: CPTC-AKR1C2-1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

AKR1C2 Antibody (CPTC-AKR1C2-1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79909)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant human full-length AKR1C2 protein (Uniprot: P52895)
• Localization: Cytoplasm
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKR1C2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 37 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for AKR1C2 Antibody (CPTC-AKR1C2-1)

• aldo-keto reductase family 1 member C2
• aldo-keto reductase family 1, member C2 (dihydrodiol dehydrogenase 2; bile acidbinding protein; 3-alpha hydroxysteroid dehydrogenase, type III)
• BABP
• Chlordecone reductase homolog HAKRD
• DD
• DD-2
• DD2DD/BABP
• DDH2FLJ53800
• Dihydrodiol dehydrogenase 2
• Dihydrodiol dehydrogenase/bile acid-binding protein
• EC 1.1.1,3-alpha-HSD3
• EC 1.1.1.213
• EC 1.3.1.20
• HAKRDAKR1C-pseudo
• HBAB
• MCDR2
• pseudo-chlordecone reductase
• Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
• type II dihydrodiol dehydrogenase
• Type III 3-alpha-hydroxysteroid dehydrogenase

Background

AKR1C2 encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKR1C2
• Uniprot:
  • P52895(Human)