Recombinant Mouse IL-9 Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-9 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using TS1 mouse helper T cells. Uyttenhove, C. et al. (1988) Proc. Natl. Acad. Sci. 85:6934. The ED50 for this effect is 0.01-0.03 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-9 protein
Gln19-Pro144
Accession #
N-terminal Sequence

No results obtained, sequencing might be blocked: Gln19 is predicted

Protein/Peptide Type
Recombinant Proteins
Gene
Il9
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
14.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
Multiple bands between 16-25 kDa, reducing conditions
Publications
Read Publications using
409-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-9 Protein, CF

  • Cytokine P40
  • HP40
  • IL9
  • IL-9
  • IL-9homolog of mouse T cell and mast cell growth factor 40
  • interleukin 9
  • interleukin-9
  • p40 cytokine
  • p40 T-cell and mast cell growth factor
  • P40
  • T-cell growth factor P40

Background

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gamma c) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gamma c to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).
  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Van Snick, J. et al. (1989) J. Exp. Med. 169:363.

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Publications for IL-9 (409-ML/CF)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.


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Bioassay
(2)
In Vivo
(3)
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Mouse
(6)
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Showing Publications 1 - 6 of 6.
Publications using 409-ML/CF Applications Species
N Liu, Y Jiang, J Chen, H Nan, Y Zhao, X Chu, A Wang, D Wang, T Qin, S Gao, Q Yi, Y Yue, S Wang IL-33 drives the antitumor effects of dendritic cells via the induction of Tc9 cells Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 30275536] (Mouse) Mouse
Robust tumor immunity to melanoma mediated by interleukin-9-producing T cells. Nat. Med., 2012;1248(0):18. 2012 [PMID: 22772464] (In Vivo, Mouse) In Vivo Mouse
Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012;109(25):9971-6. 2012 [PMID: 22665806] (Bioassay, Mouse) Bioassay Mouse
Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus. J. Virol., 2010;84(24):12703-12. 2010 [PMID: 20881038] (In Vivo, Mouse) In Vivo Mouse
Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development. J. Immunol., 2008;181(5):3285-90. 2008 [PMID: 18714000] (Bioassay, Mouse) Bioassay Mouse
Reader JR, Hyde DM, Schelegle ES, Aldrich MC, Stoddard AM, McLane MP, Levitt RC, Tepper JS Interleukin-9 induces mucous cell metaplasia independent of inflammation. Am. J. Respir. Cell Mol. Biol., 2003;28(6):664-72. 2003 [PMID: 12760964] (In Vivo, Mouse) In Vivo Mouse

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Bioinformatics

Gene Symbol Il9
Uniprot