IL-9 Antibodies

209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND₅₀ is typically 2-5 µg/mL." class="big_lightbox"> 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND₅₀ is typically 2-5 µg/mL." alt="Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF209). The ND₅₀ is typically 2-5 µg/mL." class="big_thumb" />

209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑9 Polyclonal Antibody (Catalog # AB-209-NA). The ND₅₀ is typically 20-40 µg/mL." class="big_lightbox"> 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑9 Polyclonal Antibody (Catalog # AB-209-NA). The ND₅₀ is typically 20-40 µg/mL." alt="Recombinant Human IL‑9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑9 Polyclonal Antibody (Catalog # AB-209-NA). The ND₅₀ is typically 20-40 µg/mL." class="big_thumb" />

409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-9 Monoclonal Antibody (Catalog # MAB4091). The ND₅₀ is typically 0.5-2.0 ng/mL." class="big_lightbox"> 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-9 Monoclonal Antibody (Catalog # MAB4091). The ND₅₀ is typically 0.5-2.0 ng/mL." alt="Recombinant Mouse IL-9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-9 Monoclonal Antibody (Catalog # MAB4091). The ND₅₀ is typically 0.5-2.0 ng/mL." class="big_thumb" />

409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-9 Polyclonal Antibody (Catalog # AB-409-NA). The ND₅₀ is typically 3-18 µg/mL." class="big_lightbox"> 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-9 Polyclonal Antibody (Catalog # AB-409-NA). The ND₅₀ is typically 3-18 µg/mL." alt="Recombinant Mouse IL-9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-9 Polyclonal Antibody (Catalog # AB-409-NA). The ND₅₀ is typically 3-18 µg/mL." class="big_thumb" />

4134-RL) stim-ulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Rat IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4134). The ND₅₀ is typically 1.5-7.5 µg/mL." class="big_lightbox"> 4134-RL) stim-ulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Rat IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4134). The ND₅₀ is typically 1.5-7.5 µg/mL." alt="Recombinant Rat IL-9 (Catalog # 4134-RL) stim-ulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-9 (10 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti-Rat IL-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4134). The ND₅₀ is typically 1.5-7.5 µg/mL." class="big_thumb" />

5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin." class="big_lightbox"> 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permea-bilized with saponin." class="big_thumb" />

409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF409). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF409). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Mouse IL‑9 (Catalog # 409-ML) stimulates proliferation in the TS1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑9 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF409). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

202-IL), Recombinant Human IL-4 (Catalog # 204-IL), and Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF-beta 1 (Catalog # 240-B). PVDF membrane was probed with a 0.1 µg/mL of Rabbit Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), and Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF-beta 1 (Catalog # 240-B). PVDF membrane was probed with a 0.1 µg/mL of Rabbit Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), and Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285) or (3) anti-CD3, anti-CD28, Recombinant Human IL-2 (Catalog # 202-IL), Recombinant Human IL-4 (Catalog # 204-IL), Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB285), and Recombinant Human TGF-beta 1 (Catalog # 240-B). PVDF membrane was probed with a 0.1 µg/mL of Rabbit Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB209) or (B) Mouse IgG2B Isotype Control Antibody (MAB0041) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_lightbox"> 204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB209) or (B) Mouse IgG2B Isotype Control Antibody (MAB0041) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol. " alt="Human peripheral blood mononuclear cells (PBMCs) were treated with plate-bound anti-CD3 Monoclonal Antibody (5 μg/ml) and anti-CD28 Monoclonal Antibody (2 μg/ml), Recombinant Human IL-4 (204-IL,10 ng/ml), and anti-Human IFN-gamma Monoclonal Antibody (AF-285-NA, 20 μg/ml) for 5 days and stained with (A) Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB209) or (B) Mouse IgG2B Isotype Control Antibody (MAB0041) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse Anti-Human IFN-gamma Phycoerythrin-conjugated Monoclonal Antibody (IC285P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol. " class="big_thumb" />

209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2092). The ND₅₀ is typically 1-5 µg/mL" class="big_lightbox"> 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2092). The ND₅₀ is typically 1-5 µg/mL" alt="Recombinant Human IL-9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-9 (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-9 Monoclonal Antibody (Catalog # MAB2092). The ND₅₀ is typically 1-5 µg/mL" class="big_thumb" />