Recombinant Mouse IL-33 Protein


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Applications Bioactivity

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Recombinant Mouse IL-33 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0125-0.05 ng/mL.
E. coli-derived mouse IL-33 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
20 kDa, reducing conditions
Read Publications using
3626-ML in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-33 Protein

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NF-HEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2


IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL‑1 RAcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

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Publications for IL-33 (3626-ML)(114)

We have publications tested in 2 confirmed species: Mouse, Transgenic Mouse.

We have publications tested in 8 applications: Bioassay, Cell Culture, ELISA (Standard), Flow Cytometry, In Vivo, Neutralization, Stimulation, Western Blot.

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Showing Publications 1 - 10 of 114. Show All 114 Publications.
Publications using 3626-ML Applications Species
Calafiore, M;Fu, YY;Vinci, P;Arnhold, V;Chang, WY;Jansen, SA;Egorova, A;Takashima, S;Kuttiyara, J;Ito, T;Serody, J;Nakae, S;Turnquist, H;van Es, J;Clevers, H;Lindemans, CA;Blazar, BR;Hanash, AM; A tissue-intrinsic IL-33/EGF circuit promotes epithelial regeneration after intestinal injury Nature communications 2023-09-05 [PMID: 37669929] (Bioassay, Mouse) Bioassay Mouse
Hollifield, IE;Motyka, NI;Fernando, KA;Bitoun, JP; Heat-Labile Enterotoxin Decreases Macrophage Phagocytosis of Enterotoxigenic Escherichia coli Microorganisms 2023-08-21 [PMID: 37630681] (Bioassay, Mouse) Bioassay Mouse
Hansakon, A;Jeerawattanawart, S;Angkasekwinai, P; Differential and cooperative effects of IL-25 and IL-33 on T helper cells contribute to cryptococcal virulence and brain infection Scientific reports 2023-06-19 [PMID: 37337050] (Bioassay, Mouse) Bioassay Mouse
Cho, MJ;Lee, HG;Yoon, JW;Kim, GR;Koo, JH;Taneja, R;Edelson, BT;Lee, YJ;Choi, JM; Steady-state memory-phenotype conventional CD4+ T cells exacerbate autoimmune neuroinflammation in a bystander manner via the Bhlhe40/GM-CSF axis Experimental & molecular medicine 2023-05-01 [PMID: 37121980] (Bioassay, Mouse) Bioassay Mouse
PH Papotto, B Yilmaz, G Pimenta, S Mensurado, C Cunha, GJ Fiala, D Gomes da C, N Gonçalves-, BHK Chan, B Blankenhau, RG Domingues, T Carvalho, MR Hepworth, AJ Macpherson, JE Allen, B Silva-Sant Maternal gammadelta T�cells shape offspring pulmonary type 2 immunity in a microbiota-dependent manner Cell Reports, 2023-02-07;0(0):112074. 2023-02-07 [PMID: 36787741] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
RK Gurram, D Wei, Q Yu, MJ Butcher, X Chen, K Cui, G Hu, M Zheng, X Zhu, J Oh, B Sun, JF Urban, K Zhao, WJ Leonard, J Zhu Crosstalk between ILC2s and Th2 cells varies among mouse models Cell Reports, 2023-02-02;42(2):112073. 2023-02-02 [PMID: 36735533] (Bioassay, Mouse) Bioassay Mouse
L Cheng, Y Jiao, W Jiang, X Zhang, L Zhang, G Jia IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells Journal of Immunology Research, 2022-12-16;2022(0):9543083. 2022-12-16 [PMID: 36570798] (In Vivo, Mouse, Transgenic Mouse) In Vivo Mouse, Transgenic Mouse
M Zhao, F Shao, D Yu, J Zhang, Z Liu, J Ma, P Xia, S Wang Maturation and specialization of group 2 innate lymphoid cells through the lung-gut axis Nature Communications, 2022-12-09;13(1):7600. 2022-12-09 [PMID: 36494354] (In Vivo, Mouse) In Vivo Mouse
KE Block, K Iijima, MJ Pierson, DA Walsh, R Tei, TA Kucaba, J Xu, MH Khan, C Staley, TS Griffith, HJ McSorley, H Kita, SC Jameson Physiological microbial exposure transiently inhibits mouse lung ILC2 responses to allergens Nature Immunology, 2022-11-21;0(0):. 2022-11-21 [PMID: 36411381] (In Vivo, Mouse) In Vivo Mouse
JH Badrani, AN Strohm, L Lacasa, B Civello, K Cavagnero, YA Haung, M Amadeo, LH Naji, SJ Lund, A Leng, H Kim, RE Baum, N Khorram, M Mondal, G Seumois, J Pilotte, PW Vanderklis, HM McGee, TA Doherty RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R Nature Communications, 2022-07-30;13(1):4435. 2022-07-30 [PMID: 35908044] (In Vivo, Mouse) In Vivo Mouse
Show All 114 Publications.

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Gene Symbol Il33