HAF109). A specific band was detected for IL-33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog #
FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005). View our protocol for
Staining Intracellular Molecules." class="big_lightbox" target="_blank">
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox" target="_blank">
3626-ML) stimulates proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog #
AR002). Proliferation elicited by Recombinant Mouse IL-33 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626). The ND
50 is typically 10-50 ng/mL." class="big_lightbox" target="_blank">
3626-ML) was serially diluted and captured by Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF3626) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox" target="_blank">
3626-ML) was serially diluted and captured by Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF3626) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse IL‑33 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3626) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox" target="_blank">