Recombinant Mouse IL-33 Protein, CF


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Recombinant Mouse IL-33 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0125-0.05 ng/mL.
E. coli-derived mouse IL-33 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
20 kDa, reducing conditions
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3626-ML/CF in the following applications:

Packaging, Storage & Formulations

  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-33 Protein, CF

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NF-HEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2


IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL‑1 RAcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

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Publications for IL-33 (3626-ML/CF)(61)

We have publications tested in 2 confirmed species: Mouse, Moluse.

We have publications tested in 3 applications: Bioassay, In Vivo, In vivo.

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Showing Publications 1 - 10 of 61. Show All 61 Publications.
Publications using 3626-ML/CF Applications Species
N Liu, Y Jiang, J Chen, H Nan, Y Zhao, X Chu, A Wang, D Wang, T Qin, S Gao, Q Yi, Y Yue, S Wang IL-33 drives the antitumor effects of dendritic cells via the induction of Tc9 cells Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 30275536] (Bioassay, Mouse) Bioassay Mouse
RR Ricardo-Go, SJ Van Dyken, C Schneider, J Lee, JC Nussbaum, HE Liang, D Vaka, WL Eckalbar, AB Molofsky, DJ Erle, RM Locksley Tissue signals imprint ILC2 identity with anticipatory function Nat. Immunol., 2018;0(0):. 2018 [PMID: 30201992] (Bioassay, Mouse) Bioassay Mouse
K Cao, X Liao, J Lu, S Yao, F Wu, X Zhu, D Shi, S Wen, L Liu, H Zhou IL-33/ST2 plays a critical role in endothelial cell activation and microglia-mediated neuroinflammation modulation J Neuroinflammation, 2018;15(1):136. 2018 [PMID: 29728120] (Bioassay, Mouse) Bioassay Mouse
B Sun, L Zhu, Y Tao, HX Sun, Y Li, P Wang, Y Hou, Y Zhao, X Zhang, L Zhang, N Na, Y Zhao Characterization and allergic role of IL-33-induced neutrophil polarization Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29503441] (Bioassay, Mouse) Bioassay Mouse
M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms Mucosal Immunol, 2018;11(3):615-626. 2018 [PMID: 29346348] (In Vivo, Mouse) In Vivo Mouse
J Duffen, M Zhang, K Masek-Hamm, A Nunez, A Brennan, JEC Jones, J Morin, K Nocka, M Kasaian Modulation of the IL-33/IL-13 Axis in Obesity by IL-13R?2 J. Immunol., 2018;0(0):. 2018 [PMID: 29305434] (In Vivo, Mouse) In Vivo Mouse
V Cardoso, J Chesné, H Ribeiro, B García-Cas, T Carvalho, T Bouchery, K Shah, NL Barbosa-Mo, N Harris, H Veiga-Fern Neuronal regulation of type 2 innate lymphoid cells via neuromedin U Nature, 2017;549(7671):277-281. 2017 [PMID: 28869974] (Bioassay, Mouse) Bioassay Mouse
JM Garth, KM Reeder, MS Godwin, JJ Mackel, CW Dunaway, JP Blackburn, C Steele IL-33 Signaling Regulates Innate IL-17A and IL-22 Production via Suppression of Prostaglandin E2 during Lung Fungal Infection J. Immunol., 2017;199(6):2140-2148. 2017 [PMID: 28784844] (In Vivo, Mouse) In Vivo Mouse
M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice J. Immunol., 2017;0(0):. 2017 [PMID: 28701507] (Bioassay, Mouse) Bioassay Mouse
S Taylor, Y Huang, G Mallett, C Stathopoul, TC Felizardo, MA Sun, EL Martin, N Zhu, EL Woodward, MS Elias, J Scott, NJ Reynolds, WE Paul, DH Fowler, S Amarnath PD-1 regulates KLRG1(+) group 2 innate lymphoid cells J. Exp. Med., 2017;0(0):. 2017 [PMID: 28490441] (In Vivo, Mouse) In Vivo Mouse
Show All 61 Publications.

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Blogs on IL-33.

Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory...  Read full blog post.

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Gene Symbol Il33