Recombinant Mouse IL-33 Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-33 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0125-0.05 ng/mL.
Source
E. coli-derived mouse IL-33 protein
Ser109-Ile266
Accession #
N-terminal Sequence
Ser109
Protein/Peptide Type
Recombinant Proteins
Gene
Il33
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20 kDa, reducing conditions
Publications
Read Publications using
3626-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-33 Protein, CF

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NFHEV
  • NF-HEV
  • NF-HEVNFEHEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2

Background

IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL‑1 RAcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

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Publications for IL-33 (3626-ML/CF)(58)

We have publications tested in 2 confirmed species: Mouse, Moluse.

We have publications tested in 3 applications: Bioassay, In Vivo, In vivo.


Filter By Application
Bioassay
(30)
In Vivo
(27)
In vivo
(1)
All Applications
Filter By Species
Mouse
(57)
Moluse
(1)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using 3626-ML/CF Applications Species
K Cao, X Liao, J Lu, S Yao, F Wu, X Zhu, D Shi, S Wen, L Liu, H Zhou IL-33/ST2 plays a critical role in endothelial cell activation and microglia-mediated neuroinflammation modulation J Neuroinflammation, 2018;15(1):136. 2018 [PMID: 29728120] (Bioassay, Mouse) Bioassay Mouse
B Sun, L Zhu, Y Tao, HX Sun, Y Li, P Wang, Y Hou, Y Zhao, X Zhang, L Zhang, N Na, Y Zhao Characterization and allergic role of IL-33-induced neutrophil polarization Cell. Mol. Immunol., 2018;0(0):. 2018 [PMID: 29503441] (Bioassay, Mouse) Bioassay Mouse
M Yamaguchi, SK Samuchiwal, O Quehenberg, JA Boyce, B Balestrier Macrophages regulate lung ILC2 activation via Pla2g5-dependent mechanisms Mucosal Immunol, 2018;11(3):615-626. 2018 [PMID: 29346348] (In Vivo, Mouse) In Vivo Mouse
J Duffen, M Zhang, K Masek-Hamm, A Nunez, A Brennan, JEC Jones, J Morin, K Nocka, M Kasaian Modulation of the IL-33/IL-13 Axis in Obesity by IL-13R?2 J. Immunol., 2018;0(0):. 2018 [PMID: 29305434] (In Vivo, Mouse) In Vivo Mouse
V Cardoso, J Chesné, H Ribeiro, B García-Cas, T Carvalho, T Bouchery, K Shah, NL Barbosa-Mo, N Harris, H Veiga-Fern Neuronal regulation of type 2 innate lymphoid cells via neuromedin U Nature, 2017;549(7671):277-281. 2017 [PMID: 28869974] (Bioassay, Mouse) Bioassay Mouse
M Han, C Rajput, JY Hong, J Lei, JL Hinde, Q Wu, JK Bentley, MB Hershenson The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice J. Immunol., 2017;0(0):. 2017 [PMID: 28701507] (Bioassay, Mouse) Bioassay Mouse
S Taylor, Y Huang, G Mallett, C Stathopoul, TC Felizardo, MA Sun, EL Martin, N Zhu, EL Woodward, MS Elias, J Scott, NJ Reynolds, WE Paul, DH Fowler, S Amarnath PD-1 regulates KLRG1(+) group 2 innate lymphoid cells J. Exp. Med., 2017;0(0):. 2017 [PMID: 28490441] (In Vivo, Mouse) In Vivo Mouse
F Reverchon, S Mortaud, M Sivoyon, I Maillet, A Laugeray, J Palomo, C Montécot, A Herzine, S Meme, W Meme, F Erard, B Ryffel, A Menuet, VFJ Quesniaux IL-33 receptor ST2 regulates the cognitive impairments associated with experimental cerebral malaria PLoS Pathog., 2017;13(4):e1006322. 2017 [PMID: 28448579] (Bioassay, Mouse) Bioassay Mouse
T Sezaki, Y Hirata, T Hagiwara, YI Kawamura, T Okamura, R Takanashi, K Nakano, M Tamura-Nak, LC Burkly, T Dohi Disruption of the TWEAK/Fn14 pathway prevents 5-fluorouracil-induced diarrhea in mice World J. Gastroenterol., 2017;23(13):2294-2307. 2017 [PMID: 28428709] (Bioassay, Mouse) Bioassay Mouse
M Ikutani, K Tsuneyama, M Kawaguchi, J Fukuoka, F Kudo, S Nakae, M Arita, Y Nagai, S Takaki, K Takatsu Prolonged activation of IL-5-producing ILC2 causes pulmonary arterial hypertrophy JCI Insight, 2017;2(7):e90721. 2017 [PMID: 28405615] (In Vivo, Moluse) In Vivo Moluse
Show All 58 Publications.

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Blogs on IL-33.

Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory...  Read full blog post.

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Bioinformatics

Gene Symbol Il33
Entrez
Uniprot