Recombinant Mouse IL-33 Protein, CF


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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-33 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.0125-0.05 ng/mL.
E. coli-derived mouse IL-33 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
20 kDa, reducing conditions
Read Publications using
3626-ML/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-33 Protein, CF

  • C9orf26
  • C9orf26chromosome 9 open reading frame 26 (NF-HEV)
  • DKFZp586H0523
  • DVS27
  • DVS27-related protein
  • IL1F11
  • IL-1F11
  • IL33
  • IL-33
  • interleukin 33
  • Interleukin-1 family member 11
  • interleukin-33
  • NF-HEV
  • Nuclear factor from high endothelial venules
  • RP11-575C20.2


IL-33, also known as NF-HEV and DVS 27, is a 30 kDa proinflammatory protein that may also regulate gene transcription (1‑3). DVS 27 was identifed as a gene that is up‑regulated in vasospastic cerebral arteries (1). NF-HEV was described as a nuclear factor that is preferentially expressed in the endothelial cells of high endothelial venules relative to endothelial cells from other tissues (2). IL-33 was identified based on sequence and structural homology with IL-1 family cytokines (3). DVS 27, NF-HEV, and IL-33 share 100% amino acid sequence identity. IL-33 is constitutively expressed in smooth muscle and airway epithelia. It is up‑regulated in arterial smooth muscle, dermal fibroblasts, and keratinocytes following IL-1 alpha or IL‑1 beta stimulation (1, 3). Similar to IL-1, IL-33 can be cleaved in vitro by caspase‑1, generating an N‑terminal fragment that is slightly shorter than the C‑terminal fragment (3, 4). The N‑terminal portion of full length IL-33 contains a predicted bipartite nuclear localization sequence and a homeodomain-like helix-turn-helix DNA binding domain. By immunofluorescence, full length IL-33 localizes to the nucleus in HUVECs and transfectants (2). The C‑terminal fragment, corresponding to mature IL-33, binds and triggers signaling through mast cell IL‑1 R4/ST2L, a longtime orphan receptor involved in the augmentation of Th2 cell responses (3, 5‑7). A ternary signaling complex is formed by the subsequent association of IL-33 and ST2L with IL‑1 RAcP (8). Stimulation of Th2 polarized lymphocytes with mature IL-33 in vitro induces IL-5 and IL-13 secretion (3). In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues (3). Full length and mature mouse IL-33 share approximately 55% and 90% aa sequence identity with human and rat IL-33, respectively. Mouse IL-33 shares less than 25% aa sequence identity with other IL-1 family proteins.

  1. Onda, H. et al. (1999) J. Cereb. Blood Flow Metab. 19:1279.
  2. Baekkevold, E.S. et al. (2003) Am. J. Pathol. 163:69.
  3. Schmitz, J. et al. (2005) Immunity 23:479.
  4. Black, R.A. et al. (1989) J. Biol. Chem. 264:5323.
  5. Xu, D. et al. (1998) J. Exp. Med. 187:787.
  6. Lohning, M. et al. (1998) Proc. Natl. Acad. Sci. 95:6930.
  7. Dinarello, C.A. (2005) Immunity 23:461.
  8. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.

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Publications for IL-33 (3626-ML/CF)(106)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Bioassay, Cell Culture, Flow Cytometry, In Vivo, Neutralization, Stimulation.

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Showing Publications 1 - 10 of 106. Show All 106 Publications.
Publications using 3626-ML/CF Applications Species
KE Block, K Iijima, MJ Pierson, DA Walsh, R Tei, TA Kucaba, J Xu, MH Khan, C Staley, TS Griffith, HJ McSorley, H Kita, SC Jameson Physiological microbial exposure transiently inhibits mouse lung ILC2 responses to allergens Nature Immunology, 2022;0(0):. 2022-01-01 [PMID: 36411381] (In Vivo, Mouse) In Vivo Mouse
JH Badrani, AN Strohm, L Lacasa, B Civello, K Cavagnero, YA Haung, M Amadeo, LH Naji, SJ Lund, A Leng, H Kim, RE Baum, N Khorram, M Mondal, G Seumois, J Pilotte, PW Vanderklis, HM McGee, TA Doherty RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R Nature Communications, 2022;13(1):4435. 2022-01-01 [PMID: 35908044] (In Vivo, Mouse) In Vivo Mouse
HS Lee, HW Park IL-23 plays a significant role in the augmentation of particulate matter-mediated allergic airway inflammation Journal of Cellular and Molecular Medicine, 2022;0(0):. 2022-01-01 [PMID: 35801505] (Bioassay, Mouse) Bioassay Mouse
A Sheikh, J Lu, E Melese, JH Seo, N Abraham IL-7 induces type 2 cytokine response in lung ILC2s and regulates GATA3 and CD25 expression Journal of leukocyte biology, 2022;0(0):. 2022-01-01 [PMID: 35603486] (Stimulation, Mouse) Stimulation Mouse
T Doke, A Abedini, DL Aldridge, YW Yang, J Park, CM Hernandez, MS Balzer, R Shrestra, G Coppock, JMI Rico, SY Han, J Kim, S Xin, AM Piliponsky, M Angelozzi, V Lefebvre, MC Siracusa, CA Hunter, K Susztak Single-cell analysis identifies the interaction of altered renal tubules with basophils orchestrating kidney fibrosis Nature Immunology, 2022;0(0):. 2022-01-01 [PMID: 35552540] (Bioassay, Mouse) Bioassay Mouse
F Cardoso, RGJ Klein Wolt, C Godinho-Si, RG Domingues, H Ribeiro, JA da Silva, I Mahú, AI Domingos, H Veiga-Fern Neuro-mesenchymal units control ILC2 and obesity via a brain-adipose circuit Nature, 2021;0(0):. 2021-01-01 [PMID: 34408322] (Bioassay, Mouse) Bioassay Mouse
Y Suzuki, T Hayashi, R Yokoyama, F Nakagawa, J Inoue, T Higashi, R Onodera, K Motoyama Fasting impairs type 2 helper T cell infiltration in the lung of an eosinophilic asthma mouse model FEBS Open Bio, 2021;0(0):. 2021-01-01 [PMID: 34363652] (Bioassay, Mouse) Bioassay Mouse
S Patial, BW Lewis, T Vo, I Choudhary, K Paudel, Y Mao, D Singamsett, F Brombacher, Y Saini Myeloid-IL4Ralpha is an indispensable link in IL-33-ILCs-IL-13-IL4Ralpha axis of eosinophil recruitment in murine lungs Scientific Reports, 2021;11(1):15465. 2021-01-01 [PMID: 34326406] (Bioassay, Mouse) Bioassay Mouse
P de Lucía F, C Sherwood, I Saranchova, W Xia, L Munro, CG Pfeifer, JM Piret, WA Jefferies Serum free culture for the expansion and study of type 2 innate lymphoid cells Scientific Reports, 2021;11(1):12233. 2021-01-01 [PMID: 34112824] (In Vivo, Mouse) In Vivo Mouse
D Morichika, A Taniguchi, N Oda, U Fujii, S Senoo, J Itano, A Kanehiro, Y Kitaguchi, M Yasuo, M Hanaoka, T Satoh, S Akira, K Kiura, Y Maeda, N Miyahara Loss of IL-33 enhances elastase-induced and cigarette smoke extract-induced emphysema in mice Respiratory Research, 2021;22(1):150. 2021-01-01 [PMID: 33992109] (Bioassay, Mouse) Bioassay Mouse
Show All 106 Publications.

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Interleukin 33 (IL-33) - A dual function cytokine
IL-33 is a member of the interleukin family of cytokines that regulates a wide variety of cellular functions. Its receptor is ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling and the IL-1R accessory ...  Read full blog post.

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Gene Symbol Il33