Recombinant Human TSLP Protein

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1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human TSLP (Catalog # 1398‑TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50 for this effect is 0.05-0.3 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TSLP Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. Reche, P.A. et al. (2001) J. Immunol. 167:336. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived human TSLP protein
Tyr29-Gln159, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TSLP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1398-TS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSLP Protein

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  5. Quentmeier, H. et al. (2001) Leukemia 15:1286.

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1398-TS
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Applications: Bioactivity

Publications for TSLP (1398-TS)(34)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Bioassay, Elispot Development.


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Bioassay
(33)
Elispot Development
(1)
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Human
(33)
Mouse
(1)
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using 1398-TS Applications Species
B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils Oncol Lett, 2017;14(6):7483-7488. 2017 [PMID: 29344192] (Bioassay, Human) Bioassay Human
A Orellana, V García-Gon, R López, S Pascual-Gu, E Lozoya, J Díaz, D Casals, A Barrena, S Paris, M Andrés, V Segarra, D Vilella, R Malhotra, P Eastwood, A Planagumà, M Miralpeix, A Nueda Application of a phenotypic drug discovery strategy to identify biological and chemical starting points for inhibition of TSLP production in lung epithelial cells PLoS ONE, 2018;13(1):e0189247. 2018 [PMID: 29320511] (Bioassay, Human) Bioassay Human
JT Schroeder, AP Bieneman Activation of Human Basophils by A549 Lung Epithelial Cells Reveals a Novel IgE-Dependent Response Independent of Allergen J. Immunol., 2017;0(0):. 2017 [PMID: 28652400] (Bioassay, Human) Bioassay Human
Y Peng, K Meng, L Jiang, Y Zhong, Y Yang, Y Lan, Q Zeng, L Cheng Thymic stromal lymphopoietin-induced HOTAIR activation promotes endothelial cell proliferation and migration in atherosclerosis Biosci. Rep., 2017;37(4):. 2017 [PMID: 28615347] (Bioassay, Human) Bioassay Human
V Lombardi, S Luce, H Moussu, L Morizur, C Gueguen, C Neukirch, S Chollet-Ma, L Mascarell, M Aubier, V Baron-Bodo, P Moingeon Effector and regulatory dendritic cells display distinct patterns of miRNA expression Immun Inflamm Dis, 2017;0(0):. 2017 [PMID: 28497578] (Bioassay, Human) Bioassay Human
JA Poposki, AI Klingler, BK Tan, P Soroosh, H Banie, G Lewis, KE Hulse, WW Stevens, AT Peters, LC Grammer, RP Schleimer, KC Welch, SS Smith, DB Conley, JR Raviv, JG Karras, O Akbari, RC Kern, A Kato Group 2 innate lymphoid cells are elevated and activated in chronic rhinosinusitis with nasal polyps Immun Inflamm Dis, 2017;0(0):. 2017 [PMID: 28474861] (Bioassay, Human) Bioassay Human
CY Chiu, SC Su, WL Fan, SH Lai, MH Tsai, SH Chen, KS Wong, WH Chung Whole-Genome Sequencing of a Family with Hereditary Pulmonary Alveolar Proteinosis Identifies a Rare Structural Variant Involving CSF2RA/CRLF2/IL3RA Gene Disruption Sci Rep, 2017;7(0):43469. 2017 [PMID: 28233860] (Bioassay, Human) Bioassay Human
Distinct roles of short and long thymic stromal lymphopoietin isoforms in house dust mite-induced asthmatic airway epithelial barrier disruption Sci Rep, 2016;6(0):39559. 2016 [PMID: 27996052] (Bioassay, Human) Bioassay Human
Genetic, immunological, and clinical features of patients with bacterial and fungal infections due to inherited IL-17RA deficiency Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):. 2016 [PMID: 27930337] (Bioassay, Human) Bioassay Human
Brigitte Wildemann Myeloid dendritic cells exhibit defects in activation and function in patients with multiple sclerosis J. Neuroimmunol., 2016;301(0):53-60. 2016 [PMID: 27836182] (Bioassay, Human) Bioassay Human
Show All 34 Publications.

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Bioinformatics

Gene Symbol TSLP
Entrez
Uniprot