Recombinant Human TSLP Protein

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1 μg/lane of Recombinant Human TSLP was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human TSLP (Catalog # 1398‑TS) stimulates cell proliferation in the BaF3 mouse pro-B cell line transfected with human IL-7 R alpha and human TSLP R. The ED50 for this effect is 0.05-0.3 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TSLP Protein Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells co-transfected with human IL-7 R alpha and human TSLP R. Reche, P.A. et al. (2001) J. Immunol. 167:336. The ED50 for this effect is 0.05-0.3 ng/mL.
Source
E. coli-derived human TSLP protein
Tyr29-Gln159, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
TSLP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1398-TS in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TSLP Protein

  • thymic stromal lymphopoietin
  • TSLP

Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells (1 - 3). The activities of mouse TSLP overlap with, but are distinct from, those of mouse IL-7. Both mouse TSLP and IL-7 can co-stimulate growth of thymocytes and mature T cells, and support B lymphopoiesis in long-term cultures of fetal liver cells and bone-marrow cells. Whereas mouse IL-7 facilitates the development of B220+/IgM- pre-B cells, mouse TSLP promotes the development B220+/IgM+ B cells. Human TSLP was reported to preferentially stimulate myeloid cells; inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c+ dendritic cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Within the mature region, six of the seven cysteine residues present in the mouse TSLP involved in intramolecular disulfide bond formation are conserved in the human TSLP. Human TSLP shares approximately 43% aa sequence identity with mouse TSLP. By Northern blot analysis, human TSLP expression has been detected in many tissues with the highest expressions in heart, liver, testis and prostate. TSLP signals through a heterodimeric receptor complex that consists of IL-7 R alpha and the TSLP R, a member of the hemopoietin receptor family most closely related to R gamma c.

  1. Sims, J.E. et al. (2000) J. Exp. Med. 192:671.
  2. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  3. Pandey, A. et al. (2000) Nature Immunol. 1:59.
  4. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  5. Quentmeier, H. et al. (2001) Leukemia 15:1286.

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Publications for TSLP (1398-TS)(46)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, ELISpot Development.


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Bioassay
(42)
Cell Culture
(1)
ELISpot Development
(1)
All Applications
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Human
(45)
Mouse
(1)
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Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using 1398-TS Applications Species
JH Lim, HY Kim, HG Kang, HJ Jeong, HM Kim RANKL down-regulates the mast cell proliferation through inducing senescence Cytokine, 2022;159(0):156018. 2022 [PMID: 36054965] (Bioassay, Human) Bioassay Human
C Yu, W Huang, Z Zhou, S Liang, Z Zhou, J Liu, H Zhao, L Liu, H Dong, F Zou, S Cai Short isoform thymic stromal lymphopoietin reduces inflammation and aerobic glycolysis of asthmatic airway epithelium by antagonizing long isoform thymic stromal lymphopoietin Respiratory Research, 2022;23(1):75. 2022 [PMID: 35351157] (Bioassay, Human) Bioassay Human
SR Bonam, C Chauvin, MJ Mathew, J Bayry IFN-gamma Induces PD-L1 Expression in Primed Human Basophils Cells, 2022;11(5):. 2022 [PMID: 35269423] (Bioassay, Human) Bioassay Human
L Karpf, C Trichot, L Faucheux, I Legbre, M Grandclaud, C Lahoute, H Mattoo, B Pasquier, V Soumelis A multivariate modeling framework to quantify immune checkpoint context-dependent stimulation on T cells Cell Discovery, 2022;8(1):1. 2022 [PMID: 34983927] (Bioassay, Human) Bioassay Human
KR Thomas, EJ Allenspach, ND Camp, MN Wray-Dutra, S Khim, A Zielinska-, AE Timms, JP Loftus, HD Liggitt, K Georgopoul, SK Tasian, RG James, DJ Rawlings Activated interleukin-7 receptor signaling drives B-cell acute lymphoblastic leukemia in mice Leukemia, 2021;0(0):. 2021 [PMID: 34193976] (Bioassay, Human) Bioassay Human
T Petrova, J Pesic, K Pardali, M Gaestel, JSC Arthur p38 MAPK signalling regulates cytokine production in IL-33 stimulated Type 2 Innate Lymphoid cells Sci Rep, 2020;10(1):3479. 2020 [PMID: 32103032] (Human) Human
N Ogasawara, JA Poposki, AI Klingler, BK Tan, KE Hulse, WW Stevens, AT Peters, LC Grammer, KC Welch, SS Smith, DB Conley, JR Raviv, P Soroosh, KI Takano, T Himi, RC Kern, RP Schleimer, A Kato Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps Mucosal Immunol, 2020;13(1):86-95. 2020 [PMID: 31641233] (Cell Culture, Human) Cell Culture Human
E Ragonnaud, K Moritoh, M Bodogai, F Gusev, S Garaud, C Chen, X Wang, T Baljinnyam, KG Becker, RW Maul, K Willard-Ga, E Rogaev, A Biragyn Tumor-derived Thymic stromal lymphopoietin expands bone marrow B-cell precursors in circulation to support metastasis Cancer Res., 2019;0(0):. 2019 [PMID: 31575547] (Bioassay, Human) Bioassay Human
BB Park, JW Choi, D Park, D Choi, J Paek, HJ Kim, SY Son, AU Mushtaq, H Shin, SH Kim, Y Zhou, T Lim, JY Park, JY Baek, K Kim, H Kwon, SH Son, KY Chung, HJ Jeong, HM Kim, YW Jung, K Lee, KY Lee, Y Byun, YH Jeon Structure-Activity Relationships of Baicalein and its Analogs as Novel TSLP Inhibitors Sci Rep, 2019;9(1):8762. 2019 [PMID: 31217492] (Bioassay, Human) Bioassay Human
T Hochdörfer, C Winkler, K Pardali, J Mjösberg Expression of c-Kit discriminates between two functionally distinct subsets of human type 2 innate lymphoid cells Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30892687] (Bioassay, Human) Bioassay Human
Show All 46 Publications.

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Bioinformatics

Gene Symbol TSLP
Uniprot