Recombinant Mouse IL-17A Protein

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA

Order Details

Recombinant Mouse IL-17A Protein Summary

Additional Information
A new and improved rmIL-17A is now available! It is CHO expressed and has a lower endotoxin spec!
New Product 7956-ML
Details of Functionality
Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED50 for this effect is 0.25-1.25 ng/mL.
Source
E. coli-derived
Thr22-Ala158
Accession #
N-terminal Sequence
Thr22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il17a
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15.5 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
421-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-17A Protein

  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL‑17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).

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Species: Mu
Applications: BA

Publications for (64)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bacteria.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(33)
ELISA Standard
(2)
In Vivo
(27)
All Applications
Filter By Species
Human
(1)
Mouse
(59)
Rat
(1)
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 421-ML Applications Species
A Hammer, A Schliep, S Jörg, A Haghikia, R Gold, M Kleinewiet, DN Müller, RA Linker Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation J Neuroinflammation, 2017;14(1):184. 2017 [PMID: 28899400] (Bioassay, Mouse) Bioassay Mouse
Y Arima, T Ohki, N Nishikawa, K Higuchi, M Ota, Y Tanaka, J Nio-Kobaya, M Elfeky, R Sakai, Y Mori, T Kawamoto, A Stofkova, Y Sakashita, Y Morimoto, M Kuwatani, T Iwanaga, Y Yoshioka, N Sakamoto, A Yoshimura, M Takiguchi, S Sakoda, M Prinz, D Kamimura, M Murakami Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit Elife, 2017;6(0):. 2017 [PMID: 28809157] (In Vivo, Mouse) In Vivo Mouse
T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity Cell Rep, 2017;18(13):3078-3090. 2017 [PMID: 28355561] (In Vivo, Mouse) In Vivo Mouse
AB Roos, M Mori, HK Gura, A Lorentz, L Bjermer, HJ Hoffmann, JS Erjefält, MR Stampfli Increased IL-17RA and IL-17RC in End-Stage COPD and the Contribution to Mast Cell Secretion of FGF-2 and VEGF Respir. Res, 2017;18(1):48. 2017 [PMID: 28298222] (Bioassay, Mouse) Bioassay Mouse
Surface-bound bovine serum albumin carrier protein as present in recombinant cytokine preparations amplifies T helper 17 cell polarization Sci Rep, 2016;6(0):36598. 2016 [PMID: 27808281] (Bioassay, Mouse) Bioassay Mouse
Synovium-Derived MicroRNAs Regulate Bone Pathways in Rheumatoid Arthritis J Bone Miner Res, 2016;0(0):. 2016 [PMID: 27676131] (Bioassay, Mouse) Bioassay Mouse
Breakpoint Cluster Region-Mediated Inflammation Is Dependent on Casein Kinase II J Immunol, 2016;0(0):. 2016 [PMID: 27630163] (Bioassay, Human) Bioassay Human
Daniel Hsu CF Lung Infection and Inflammation: IL-17 Pathophysiology and Therapeutic Intervention Infect Immun, 2016;0(0):. 2016 [PMID: 27271746] (In Vivo, Mouse) In Vivo Mouse
Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016;6(0):26851. 2016 [PMID: 27246365] (Bioassay, Mouse) Bioassay Mouse
Miljkovic D, Blazevski J, Petkovic F, Djedovic N, Momcilovic M, Stanisavljevic S, Jevtic B, Mostarica Stojkovic M, Spasojevic I A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate. J Immunol, 2015;194(6):2493-503. 2015 [PMID: 25681336] (Bioassay, Rat) Bioassay Rat
Show All 64 Publications.

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FAQs for . (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol Il17a
Entrez
Uniprot