Recombinant Mouse IL-17A Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse IL-17A Protein Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED 50 for this effect is 0.25-1.25 ng/mL.
• Source: E. coli-derived mouse IL-17/IL-17A protein Thr22-Ala158
• Accession #: Q62386.1
• N-terminal Sequence: Thr22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Il17a
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 15.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 25 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-17A Protein

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature mouse IL-17A shares 61% and 89% amino acid sequence identity with human and rat IL-17A, respectively (3, 4). IL-17A is secreted by Th17 cells, gamma /δ T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides (9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (Fossiez, 14). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (15, 16). IL-17A has been shown to exert either tumorigenic or anti-tumor effects (17, 18).

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Yao, Z. et al. (1996) Gene 168:223.
4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.
5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
8. Rong, Z. et al. (2009) Cell Res. 19:208.
9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
13. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
14. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
15. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
16. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
17. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
18. Kryczek, I. et al. (2009) Blood 114:357.

Publications for IL-17/IL-17A (421-ML)(92)

Publications using 421-ML

• Zhang, H;Xue, K;Li, W;Yang, X;Gou, Y;Su, X;Qian, F;Sun, L; Cullin5 drives experimental asthma exacerbations by modulating alveolar macrophage antiviral immunity Nature communications 2024-01-04 [PMID: 38177117] (Bioassay, Mouse)
• X Wang, S Wu, W Wu, W Zhang, L Li, Q Liu, Z Yan Candida albicans Promotes Oral Cancer via IL-17A/IL-17RA-Macrophage Axis MBio, 2023-04-17;0(0):e0044723. 2023-04-17 [PMID: 37067414] (Bioassay, Mouse)
• J Youwakim, D Vallerand, H Girouard Neurovascular Coupling in Hypertension Is Impaired by IL-17A through Oxidative Stress International Journal of Molecular Sciences, 2023-02-16;24(4):. 2023-02-16 [PMID: 36835372] (In Vivo, Mouse, Transgenic Mouse)
• Y Liu, Y Meng, C Zhou, J Yan, C Guo, W Dong Activation of the IL-17/TRAF6/NF-kappaB pathway is implicated in Abeta-induced neurotoxicity Bmc Neuroscience, 2023-02-23;24(1):14. 2023-02-23 [PMID: 36823558] (Mouse)
• FP Veras, GA Publio, BM Melo, DS Prado, T Norbiato, NT Cecilio, C Hiroki, LEA Damasceno, R Jung, JE Toller-Kaw, TV Martins, SF Assunção, D Lima, MG Alves, GV Vieira, LA Tavares, ALR Alves-Reze, SH Karbach, HI Nakaya, TM Cunha, CS Souza, FQ Cunha, KU Sales, A Waisman, JC Alves-Filh Pyruvate kinase M2 mediates IL-17 signaling in keratinocytes driving psoriatic skin inflammation Cell Reports, 2022-12-27;41(13):111897. 2022-12-27 [PMID: 36577385] (Bioassay, Human)
• Y Li, M Mao, L Zhu, Q Sun, J Tong, Z Zhou IL-17A drives cognitive aging probably via inducing neuroinflammation and theta oscillation disruption in the hippocampus Oncogene, 2022-05-31;108(0):108898. 2022-05-31 [PMID: 35729833] (In Vivo, Mouse)
• M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis Cell Reports, 2021-12-07;37(10):110094. 2021-12-07 [PMID: 34879272] (Bioassay, Mouse)
• KJ Roberts, MF Cubitt, TM Carlton, L Rodrigues-, L Maggiore, R Chai, S Clare, K Harcourt, TT MacDonald, KP Ray, A Vossenkämp, MR West, JS Crowe Preclinical development of a bispecific TNFalpha/IL-23 neutralising domain antibody as a novel oral treatment for inflammatory bowel disease Scientific Reports, 2021-09-30;11(1):19422. 2021-09-30 [PMID: 34593832] (ELISA Standard, N/A)
• LM Sanmarco, MA Wheeler, C Gutiérrez-, CM Polonio, M Linnerbaue, FA Pinho-Ribe, Z Li, F Giovannoni, KV Batterman, G Scalisi, SEJ Zandee, ES Heck, M Alsuwailm, DL Rosene, B Becher, IM Chiu, A Prat, FJ Quintana Gut-licensed IFN&amp;gamma+ NK cells drive LAMP1+TRAIL+ anti-inflammatory astrocytes Nature, 2021-01-06;0(0):. 2021-01-06 [PMID: 33408417] (Bioassay, Mouse)
• G Gupta, Z Mou, P Jia, R Sharma, R Zayats, SM Viana, L Shan, A Barral, VS Boaventura, TT Murooka, A Soussi-Gou, CI de Oliveir, JE Uzonna The Long Pentraxin 3 (PTX3) Suppresses Immunity to Cutaneous Leishmaniasis by Regulating CD4+ T Helper Cell Response Cell Reports, 2020-12-15;33(11):108513. 2020-12-15 [PMID: 33326783] (Bioassay, Transgenic Mouse)
• T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death Cell. Signal., 2020-06-15;73(0):109690. 2020-06-15 [PMID: 32553549] (Bioassay, Mouse)
• M Li, C Li, X Wu, T Chen, L Ren, B Xu, J Cao Microbiota-driven interleukin-17 production provides immune protection against invasive candidiasis Crit Care, 2020-05-27;24(1):268. 2020-05-27 [PMID: 32460890] (In Vivo, Mouse)
• H Koriyama, Y Ikeda, H Nakagami, M Shimamura, S Yoshida, H Rakugi, R Morishita Development of an IL-17A DNA Vaccine to Treat Systemic Lupus Erythematosus in Mice Vaccines (Basel), 2020-02-12;8(1):. 2020-02-12 [PMID: 32059488] (Bioassay, Mouse)
• J Li, P Kong, C Chen, J Tang, X Jin, J Yan, Y Wang Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis Oxid Med Cell Longev, 2019-08-18;2019(0):1475729. 2019-08-18 [PMID: 31531179] (In Vivo, Mouse)
• C Cataisson, R Salcedo, AM Michalowsk, M Klosterman, S Naik, L Li, MJ Pan, A Sweet, JQ Chen, LG Kostecka, M Karwan, L Smith, RM Dai, CA Stewart, L Lyakh, WT Hsieh, A Khan, H Yang, M Lee, G Trinchieri, SH Yuspa T cell deletion of MyD88 connects IL-17 and IkBz to RAS oncogenesis Mol. Cancer Res., 2019-06-04;0(0):. 2019-06-04 [PMID: 31164412] (Bioassay, Mouse, Transgenic Mouse)
• A Zychlinsky, M Rousseau, L Lacerda Ma, T Canton, CR Consiglio, ML Albert, M Fontes, D Duffy, MA Ingersoll Sex differences in IL-17 contribute to chronicity in male versus female urinary tract infection JCI Insight, 2019-05-30;5(0):. 2019-05-30 [PMID: 31145099] (In Vivo, Mouse)
• H Katsura, Y Kobayashi, PR Tata, BLM Hogan IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration Stem Cell Reports, 2019-03-28;0(0):. 2019-03-28 [PMID: 30930244] (Bioassay, Mouse)
• B Zwarycz, AD Gracz, KR Rivera, IA Williamson, LA Samsa, J Starmer, MA Daniele, L Salter-Cid, Q Zhao, ST Magness IL22 Inhibits Epithelial Stem Cell Expansion in an Ileal Organoid Model Cell Mol Gastroenterol Hepatol, 2018-07-04;7(1):1-17. 2018-07-04 [PMID: 30364840] (Bioassay, Mouse)
• Y Okuyama, Y Tanaka, JJ Jiang, D Kamimura, A Nakamura, M Ota, T Ohki, D Higo, H Ogura, N Ishii, T Atsumi, M Murakami Bmi1 Regulates I?B? Degradation via Association with the SCF Complex J. Immunol., 2018-09-12;0(0):. 2018-09-12 [PMID: 30209188] (Bioassay, Mouse)
• Y Tanaka, L Sabharwal, M Ota, I Nakagawa, JJ Jiang, Y Arima, H Ogura, M Okochi, M Ishii, D Kamimura, M Murakami Presenilin 1 Regulates NF-?B Activation via Association with Breakpoint Cluster Region and Casein Kinase II J. Immunol., 2018-09-10;0(0):. 2018-09-10 [PMID: 30201812] (Bioassay, In Vivo, Mouse)
• M Bambouskov, L Gorvel, V Lampropoul, A Sergushich, E Loginichev, K Johnson, D Korenfeld, ME Mathyer, H Kim, LH Huang, D Duncan, H Bregman, A Keskin, A Santeford, RS Apte, R Sehgal, B Johnson, GK Amarasingh, MP Soares, T Satoh, S Akira, T Hai, C de Guzman, K Auclair, TP Roddy, SA Biller, M Jovanovic, E Klechevsky, KM Stewart, GJ Randolph, MN Artyomov Electrophilic properties of itaconate and derivatives regulate the�I?B?-ATF3 inflammatory axis Nature, 2018-04-18;556(7702):501-504. 2018-04-18 [PMID: 29670287] (Bioassay, Mouse)
• L Markey, L Shaban, ER Green, KP Lemon, J Mecsas, CA Kumamoto Pre-colonization with the commensal fungus Candida albicans reduces murine susceptibility to Clostridium difficile infection Gut Microbes, 2018-05-30;0(0):0. 2018-05-30 [PMID: 29667487] (In Vivo, Mouse)
• AS Chitre, MG Kattah, YY Rosli, M Pao, M Deswal, SG Deeks, PW Hunt, M Abdel-Mohs, LJ Montaner, CC Kim, A Ma, M Somsouk, JM McCune A20 upregulation during treated HIV disease is associated with intestinal epithelial cell recovery and function PLoS Pathog., 2018-03-05;14(3):e1006806. 2018-03-05 [PMID: 29505600] (Bioassay, Mouse)
• C Navarrete, F Carrillo-S, B Palomares, M Mecha, C Jiménez-Ji, L Mestre, A Feliú, ML Bellido, BL Fiebich, G Appendino, MA Calzado, C Guaza, E Muñoz Hypoxia mimetic activity of VCE-004.8, a cannabidiol quinone derivative: implications for multiple sclerosis therapy J Neuroinflammation, 2018-03-01;15(1):64. 2018-03-01 [PMID: 29495967] (Bioassay, Mouse)
• A Mancini, M Tantucci, P Mazzocchet, A de Iure, V Durante, L Macchioni, C Giampà, A Alvino, L Gaetani, C Costa, A Tozzi, P Calabresi, M Di Filippo Microglial activation and the nitric oxide/cGMP/PKG pathway underlie enhanced neuronal vulnerability to mitochondrial dysfunction in experimental multiple sclerosis Neurobiol. Dis., 2018-01-09;0(0):. 2018-01-09 [PMID: 29325869] (Bioassay, Mouse)
• A Hammer, A Schliep, S Jörg, A Haghikia, R Gold, M Kleinewiet, DN Müller, RA Linker Impact of combined sodium chloride and saturated long-chain fatty acid challenge on the differentiation of T helper cells in neuroinflammation J Neuroinflammation, 2017-09-12;14(1):184. 2017-09-12 [PMID: 28899400] (Bioassay, Mouse)
• Y Arima, T Ohki, N Nishikawa, K Higuchi, M Ota, Y Tanaka, J Nio-Kobaya, M Elfeky, R Sakai, Y Mori, T Kawamoto, A Stofkova, Y Sakashita, Y Morimoto, M Kuwatani, T Iwanaga, Y Yoshioka, N Sakamoto, A Yoshimura, M Takiguchi, S Sakoda, M Prinz, D Kamimura, M Murakami Brain micro-inflammation at specific vessels dysregulates organ-homeostasis via the activation of a new neural circuit Elife, 2017-08-15;6(0):. 2017-08-15 [PMID: 28809157] (In Vivo, Mouse)
• T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity Cell Rep, 2017-03-28;18(13):3078-3090. 2017-03-28 [PMID: 28355561] (In Vivo, Mouse)
• AB Roos, M Mori, HK Gura, A Lorentz, L Bjermer, HJ Hoffmann, JS Erjefält, MR Stampfli Increased IL-17RA and IL-17RC in End-Stage COPD and the Contribution to Mast Cell Secretion of FGF-2 and VEGF Respir. Res, 2017-03-15;18(1):48. 2017-03-15 [PMID: 28298222] (Bioassay, Mouse)
• David A Hildeman Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire J. Immunol., 2016-11-16;0(0):. 2016-11-16 [PMID: 27852740] (Bioassay, Mouse)
• Surface-bound bovine serum albumin carrier protein as present in recombinant cytokine preparations amplifies T helper 17 cell polarization Sci Rep, 2016-11-03;6(0):36598. 2016-11-03 [PMID: 27808281] (Bioassay, Mouse)
• Synovium-Derived MicroRNAs Regulate Bone Pathways in Rheumatoid Arthritis J Bone Miner Res, 2016-10-28;0(0):. 2016-10-28 [PMID: 27676131] (Bioassay, Mouse)
• Breakpoint Cluster Region-Mediated Inflammation Is Dependent on Casein Kinase II J Immunol, 2016-09-14;0(0):. 2016-09-14 [PMID: 27630163] (Bioassay, Human)
• Daniel Hsu CF Lung Infection and Inflammation: IL-17 Pathophysiology and Therapeutic Intervention Infect Immun, 2016-08-19;0(0):. 2016-08-19 [PMID: 27271746] (In Vivo, Mouse)
• Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (Bioassay, Mouse)
• Reynolds J, Lee Y, Shi Y, Wang X, Angkasekwinai P, Nallaparaju K, Flaherty S, Chang S, Watarai H, Dong C Interleukin-17B Antagonizes Interleukin-25-Mediated Mucosal Inflammation. Immunity, 2015-04-14;42(4):692-703. 2015-04-14 [PMID: 25888259] (Bioassay, Mouse)
• Miljkovic D, Blazevski J, Petkovic F, Djedovic N, Momcilovic M, Stanisavljevic S, Jevtic B, Mostarica Stojkovic M, Spasojevic I A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate. J Immunol, 2015-02-13;194(6):2493-503. 2015-02-13 [PMID: 25681336] (Bioassay, Rat)
• Harada M, Kamimura D, Arima Y, Kohsaka H, Nakatsuji Y, Nishida M, Atsumi T, Meng J, Bando H, Singh R, Sabharwal L, Jiang J, Kumai N, Miyasaka N, Sakoda S, Yamauchi-Takihara K, Ogura H, Hirano T, Murakami M Temporal expression of growth factors triggered by epiregulin regulates inflammation development. J Immunol, 2015-01-02;194(3):1039-46. 2015-01-02 [PMID: 25556244] (Bioassay, In Vivo, Mouse)
• Dallenbach K, Maurer P, Rohn T, Zabel F, Kopf M, Bachmann M Protective effect of a germline, IL-17-neutralizing antibody in murine models of autoimmune inflammatory disease. Eur J Immunol, 2015-01-23;45(4):1238-47. 2015-01-23 [PMID: 25545966] (Bioassay, Mouse)
• Ha H, Wang H, Pisitkun P, Kim J, Tassi I, Tang W, Morasso M, Udey M, Siebenlist U IL-17 drives psoriatic inflammation via distinct, target cell-specific mechanisms. Proc Natl Acad Sci U S A, 2014-08-04;111(33):E3422-31. 2014-08-04 [PMID: 25092341] (Bioassay, Mouse)
• Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile. BMC Biol, 2014-05-09;12(0):32. 2014-05-09 [PMID: 24886643] (Bioassay, Mouse)
• Ardeljan , Daniel, Wang , Yujuan, Park , Stanley, Shen , Defen, Chu , Xi Kathy, Yu , Cheng-Ro, Abu-Asab , Mones, Tuo , Jingshen, Eberhart , Charles, Olsen , Timothy, Mullins , Robert F, White , Gary, Wadsworth , Sam, Scaria , Abraham, Chan , Chi-Chao Interleukin-17 retinotoxicity is prevented by gene transfer of a soluble interleukin-17 receptor acting as a cytokine blocker: implications for age-related macular degeneration. PLoS ONE, 2014-04-29;9(4):e95900. 2014-04-29 [PMID: 24780906] (Bioassay, Mouse)
• Akiyama T, Miyamoto Y, Yoshimura K, Yamada A, Takami M, Suzawa T, Hoshino M, Imamura T, Akiyama C, Yasuhara R, Mishima K, Maruyama T, Kohda C, Tanaka K, Potempa J, Yasuda H, Baba K, Kamijo R Porphyromonas gingivalis-derived lysine gingipain enhances osteoclast differentiation induced by tumor necrosis factor-alpha and interleukin-1beta but suppresses that by interleukin-17A: importance of proteolytic degradation of osteoprotegerin by lysine gingipain. J Biol Chem, 2014-04-22;289(22):15621-30. 2014-04-22 [PMID: 24755218] (Bioassay, Degradation, Bacteria - Porphyromonas gingivalis, Mouse)
• Carrillo-Salinas F, Navarrete C, Mecha M, Feliu A, Collado J, Cantarero I, Bellido M, Munoz E, Guaza C A cannabigerol derivative suppresses immune responses and protects mice from experimental autoimmune encephalomyelitis. PLoS ONE, 2014-04-11;9(4):e94733. 2014-04-11 [PMID: 24727978] (Bioassay, Mouse)
• Sriskantharajah S, Guckel E, Tsakiri N, Kierdorf K, Brender C, Ben-Addi A, Veldhoen M, Tsichlis P, Stockinger B, O'Garra A, Prinz M, Kollias G, Ley S Regulation of experimental autoimmune encephalomyelitis by TPL-2 kinase. J Immunol, 2014-03-17;192(8):3518-29. 2014-03-17 [PMID: 24639351] (Bioassay, Mouse)
• Yang E, Lee J, Lee S, Kim E, Moon Y, Jung Y, Park S, Cho M EGCG attenuates autoimmune arthritis by inhibition of STAT3 and HIF-1alpha with Th17/Treg control. PLoS ONE, 2014-02-18;9(2):e86062. 2014-02-18 [PMID: 24558360] (ELISA (Standard), Mouse)
• Moon C, VanDussen K, Miyoshi H, Stappenbeck T Development of a primary mouse intestinal epithelial cell monolayer culture system to evaluate factors that modulate IgA transcytosis. Mucosal Immunol, 2013-11-13;7(4):818-28. 2013-11-13 [PMID: 24220295] (Bioassay, Mouse)
• Hwang I, Hwang K, Park C, Harrison K, Kehrl J Rgs13 constrains early B cell responses and limits germinal center sizes. PLoS ONE, 2013-03-22;8(3):e60139. 2013-03-22 [PMID: 23533672] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Barin JG, Baldeviano GC, Talor MV, Wu L, Ong S, Quader F, Chen P, Zheng D, Caturegli P, Rose NR, Cihakova D Macrophages participate in IL-17-mediated inflammation. Eur. J. Immunol., 2012-01-23;42(3):726-36. 2012-01-23 [PMID: 22161142] (Bioassay, Mouse)
• Sadik CD, Kim ND, Alekseeva E, Luster AD IL-17RA signaling amplifies antibody-induced arthritis. PLoS ONE, 2011-10-20;6(10):e26342. 2011-10-20 [PMID: 22028860] (Bioassay, Mouse)
• Kono H, Fujii H, Ogiku M, Hosomura N, Amemiya H, Tsuchiya M, Hara M Role of IL-17A in neutrophil recruitment and hepatic injury after warm ischemia-reperfusion mice. J. Immunol., 2011-09-26;187(9):4818-25. 2011-09-26 [PMID: 21949019] (In Vivo, Mouse)
• Whitehead GS, Wilson RH, Nakano K, Burch LH, Nakano H, Cook DN IL-35 production by inducible costimulator (ICOS)-positive regulatory T cells reverses established IL-17-dependent allergic airways disease. J. Allergy Clin. Immunol., 2011-09-08;129(1):207-15.e1-5. 2011-09-08 [PMID: 21906794] (In Vivo, Mouse)
• Li Z, Burns AR, Han L, Rumbaut RE, Smith CW IL-17 and VEGF are necessary for efficient corneal nerve regeneration. Am. J. Pathol., 2011-03-01;178(3):1106-16. 2011-03-01 [PMID: 21356362] (In Vivo, Mouse)
• Liu Y, Mei J, Gonzales L, Yang G, Dai N, Wang P, Zhang P, Favara M, Malcolm KC, Guttentag S, Worthen GS IL-17A and TNF-alpha Exert Synergistic Effects on Expression of CXCL5 by Alveolar Type II Cells In Vivo and In Vitro. J. Immunol., 2011-01-31;186(5):3197-205. 2011-01-31 [PMID: 21282514] (In Vivo, Mouse)
• Kagami S, Rizzo HL, Kurtz SE IL-23 and IL-17A, but not IL-12 and IL-22, are required for optimal skin host defense against Candida albicans. J. Immunol., 2010-10-04;185(9):5453-62. 2010-10-04 [PMID: 20921529] (In Vivo, Mouse)
• Wondimu Z, Santodomingo-Garzon T, Le T, Swain MG Protective role of interleukin-17 in murine NKT cell-driven acute experimental hepatitis. Am. J. Pathol., 2010-09-16;177(5):2334-46. 2010-09-16 [PMID: 20847291] (Bioassay, In Vivo, Mouse)
• Blumberg H, Dinh H, Dean C, Trueblood ES, Bailey K, Shows D, Bhagavathula N, Aslam MN, Varani J, Towne JE, Sims JE IL-1RL2 and its ligands contribute to the cytokine network in psoriasis. J. Immunol., 2010-09-10;185(7):4354-62. 2010-09-10 [PMID: 20833839] (In Vivo, Mouse)
• Yoshizaki A, Yanaba K, Iwata Y Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model. J. Immunol., 2010-07-12;185(4):2502-15. 2010-07-12 [PMID: 20624949] (Bioassay, Mouse)
• Cho JS, Pietras EM, Garcia NC, Ramos RI, Farzam DM, Monroe HR, Magorien JE, Blauvelt A, Kolls JK, Cheung AL, Cheng G, Modlin RL, Miller LS IL-17 is essential for host defense against cutaneous Staphylococcus aureus infection in mice. J. Clin. Invest., 2010-04-01;120(5):1762-73. 2010-04-01 [PMID: 20364087] (Bioassay, In Vivo, Bacteria, Mouse)
• Guilloteau K, Paris I, Pedretti N, Boniface K, Juchaux F, Huguier V, Guillet G, Bernard FX, Lecron JC, Morel F Skin Inflammation Induced by the Synergistic Action of IL-17A, IL-22, Oncostatin M, IL-1alpha, and TNF-alpha Recapitulates Some Features of Psoriasis. J. Immunol., 2010-03-24;184(0):5263-70. 2010-03-24 [PMID: 20335534] (In Vivo, Mouse)
• Ma J, Wang J, Wan J, Charboneau R, Chang Y, Barke RA, Roy S Morphine disrupts interleukin-23 (IL-23)/IL-17-mediated pulmonary mucosal host defense against Streptococcus pneumoniae infection. Infect. Immun., 2009-12-07;78(2):830-7. 2009-12-07 [PMID: 19995896] (In Vivo, Mouse)
• Moransard M, Sawitzky M, Fontana A, Suter T Expression of the HGF receptor c-met by macrophages in experimental autoimmune encephalomyelitis. Glia, 2010-04-01;58(5):559-71. 2010-04-01 [PMID: 19941340] (Bioassay, Mouse)
• Meeks KD, Sieve AN, Kolls JK, Ghilardi N, Berg RE IL-23 is required for protection against systemic infection with Listeria monocytogenes. J. Immunol., 2009-12-15;183(12):8026-34. 2009-12-15 [PMID: 19923464] (ELISA (Standard), Mouse)
• Oyoshi MK, Elkhal A, Kumar L, Scott JE, Koduru S, He R, Leung DY, Howell MD, Oettgen HC, Murphy GF, Geha RS Vaccinia virus inoculation in sites of allergic skin inflammation elicits a vigorous cutaneous IL-17 response. Proc. Natl. Acad. Sci. U.S.A., 2009-08-17;106(35):14954-9. 2009-08-17 [PMID: 19706451] (Bioassay, Mouse)
• Wilson RH, Whitehead GS, Nakano H, Free ME, Kolls JK, Cook DN Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness. Am. J. Respir. Crit. Care Med., 2009-08-06;180(8):720-30. 2009-08-06 [PMID: 19661246] (In Vivo, Mouse)
• Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum. J. Immunol., 2009-06-19;183(2):1291-300. 2009-06-19 [PMID: 19542374] (In Vivo, Mouse)
• Freitas A, Alves-Filho JC, Victoni T, Secher T, Lemos HP, Sonego F, Cunha FQ, Ryffel B IL-17 receptor signaling is required to control polymicrobial sepsis. J. Immunol., 2009-06-15;182(12):7846-54. 2009-06-15 [PMID: 19494309] (In Vivo, Mouse)
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (ELISA (Standard), In Vivo, Mouse)
• Otani K, Watanabe T, Tanigawa T, Okazaki H, Yamagami H, Watanabe K, Tominaga K, Fujiwara Y, Oshitani N, Arakawa T Anti-inflammatory effects of IL-17A on Helicobacter pylori-induced gastritis. Biochem. Biophys. Res. Commun., 2009-02-26;382(2):252-8. 2009-02-26 [PMID: 19249291] (In Vivo, Mouse)
• Ozment-Skelton TR, deFluiter EA, Ha T, Li C, Graves BM, Ferguson DA, Schweitzer JB, Preizsner J, Brown GD, Gordon S, Kalbfleisch JH, Williams DL Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. Crit. Care Med., 2009-03-01;37(3):1038-45. 2009-03-01 [PMID: 19237915] (Bioassay, Mouse)
• Swaidani S, Bulek K, Kang Z, Liu C, Lu Y, Yin W, Aronica M, Li X The critical role of epithelial-derived Act1 in IL-17- and IL-25-mediated pulmonary inflammation. J. Immunol., 2009-02-01;182(3):1631-40. 2009-02-01 [PMID: 19155512] (In Vivo, Mouse)
• Claudio E, Sonder SU, Saret S, Carvalho G, Ramalingam TR, Wynn TA, Chariot A, Garcia-Perganeda A, Leonardi A, Paun A, Chen A, Ren NY, Wang H, Siebenlist U The adaptor protein CIKS/Act1 is essential for IL-25-mediated allergic airway inflammation. J. Immunol., 2009-02-01;182(3):1617-30. 2009-02-01 [PMID: 19155511] (Bioassay, In Vivo, Mouse)
• Ishigame H, Kakuta S, Nagai T, Kadoki M, Nambu A, Komiyama Y, Fujikado N, Tanahashi Y, Akitsu A, Kotaki H, Sudo K, Nakae S, Sasakawa C, Iwakura Y Differential roles of interleukin-17A and -17F in host defense against mucoepithelial bacterial infection and allergic responses. Immunity, 2009-01-16;30(1):108-19. 2009-01-16 [PMID: 19144317] (Bioassay, Mouse)
• Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Cell Res., 2009-02-01;19(2):208-15. 2009-02-01 [PMID: 19079364] (Bioassay, Mouse)
• Maione F, Paschalidis N, Mascolo N, Dufton N, Perretti M, D'Acquisto F Interleukin 17 sustains rather than induces inflammation. Biochem. Pharmacol., 2008-11-25;77(5):878-87. 2008-11-25 [PMID: 19073154] (In Vivo, Mouse)
• Nagata T, McKinley L, Peschon JJ, Alcorn JF, Aujla SJ, Kolls JK Requirement of IL-17RA in Con A induced hepatitis and negative regulation of IL-17 production in mouse T cells. J. Immunol., 2008-12-01;181(11):7473-9. 2008-12-01 [PMID: 19017936] (Bioassay, Mouse)
• Watanabe H, Kawaguchi M, Fujishima S, Ogura M, Matsukura S, Takeuchi H, Ohba M, Sueki H, Kokubu F, Hizawa N, Adachi M, Huang SK, Iijima M Functional characterization of IL-17F as a selective neutrophil attractant in psoriasis. J. Invest. Dermatol., 2008-10-02;129(3):650-6. 2008-10-02 [PMID: 18830271] (In Vivo, Mouse)
• Rickel EA, Siegel LA, Yoon BR, Rottman JB, Kugler DG, Swart DA, Anders PM, Tocker JE, Comeau MR, Budelsky AL Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities. J. Immunol., 2008-09-15;181(6):4299-310. 2008-09-15 [PMID: 18768888] (Bioassay, Mouse)
• Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation. J. Immunol., 2008-09-15;181(6):4279-86. 2008-09-15 [PMID: 18768886] (Bioassay, Mouse)
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FAQs for IL-17/IL-17A (421-ML). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: Il17a
• Uniprot:
  • Q62386.1()