Recombinant Mouse IL-17A/F Heterodimer Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse IL-17A/F Heterodimer Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811. The ED₅₀ for this effect is 0.075-0.375 μg/mL.
• Source: E. coli-derived mouse IL-17A/F Heterodimer protein
  

  Mouse IL-17A (Thr22 - Ala158) Accession # Q62386

  Mouse IL-17F (Arg21 - Ala153, with an N-terminal Met) Accession # AAM77568
  N-terminus	C-terminus

• Accession #: Q62386 (IL-17A) & AAM77568 (IL-17F)
• N-terminal Sequence: Thr22 (IL-17A) & Met (IL-17F)
• Structure / Form: Disulfide-linked heterodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 14.9 kDa (IL-17A) & 15.5 kDa (IL-17F), reducing conditions.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-17A/F Heterodimer Protein, CF

• CTLA8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• Cytotoxic T-Lymphocyte-Associated Protein 8
• IL17
• IL-17
• IL-17A
• IL-17A/F Heterodimer
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A
• interleukin-17A

Background

IL-17A/F is an approximately 40 kDa, secreted, disulfide-linked heterodimeric glycoprotein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1). Members of this family demonstrate a structural motif termed a cysteine-knot that also characterizes a large superfamily of growth factors. Although most cysteine-knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (2 - 4). Mouse IL-17A is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 133 aa mature region. The mature chain contains one potential site of N-linked glycosylation. Mouse IL-17F is synthesized as a 161 aa precursor. Like IL-17A, IL-17F contains one potential site of N-linked glycosylation. IL-17A and IL-17F share 50% aa sequence identity. Mouse IL-17A and IL-17F are 61% and 56% identical to human IL-17A and IL-17F on the aa level, respectively. IL-17A/F and the IL-17A and IL-17F homodimers are produced by an activated subset of CD4⁺ T cells, termed Th17 (1, 5 - 7). IL-23 drives Th17 lymphocytes to produce these cytokines (5, 8 - 9). Studies have identified that the widely expressed receptors IL-17RA and IL-17RC form a heterodimer for the binding of IL-17A and IL-17F, and IL-17A/F (5, 10 - 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (6).

1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
2. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
3. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
4. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
5. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
6. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
7. Ouyang, W. et al. (2008) Immunity 28:454.
8. Steinman, L. (2007) Nat. Med. 13:139.
9. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
10. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
11. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.

Publications for IL-17A/F Heterodimer (5390-IL/CF)(3)

Publications using 5390-IL/CF

• F Zhang, O Ledue, M Jun, C Goulart, R Malley, YJ Lu Protection against Staphylococcus aureus Colonization and Infection by B- and T-Cell-Mediated Mechanisms MBio, 2018-10-16;9(5):. 2018-10-16 [PMID: 30327437] (In Vivo, Mouse)
• Keratinocytes contribute intrinsically to psoriasis upon loss of Tnip1 function Proc Natl Acad Sci USA, 2016-09-26;0(0):. 2016-09-26 [PMID: 27671649] (In Vivo, Mouse)
• Kono H, Fujii H, Ogiku M, Hosomura N, Amemiya H, Tsuchiya M, Hara M Role of IL-17A in neutrophil recruitment and hepatic injury after warm ischemia-reperfusion mice. J. Immunol., 2011-09-26;187(9):4818-25. 2011-09-26 [PMID: 21949019] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • Q62386 (IL-17A) & AAM77568 (IL-17F)()