Recombinant Mouse IL-12 Protein, CF


1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 25 kDa and 43 kDa (R) and 68 kDa (NR) .
Recombinant Mouse IL-12 (Catalog # 419‑ML/CF) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01-0.1 ng/mL.

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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IL-12 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
Accession # P43432
Mouse IL-12 p35
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23 (p40) & Arg23 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
70 kDa, non-reducing conditions
Read Publications using
419-ML/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein, CF

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems’ recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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Publications for IL-12 (419-ML/CF)(237)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Mousd, N/A, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.

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Showing Publications 1 - 10 of 237. Show All 237 Publications.
Publications using 419-ML/CF Applications Species
YH Chen, RH Liu, YZ Cui, A Hettinghou, WY Fu, L Zhang, C Zhang, CJ Liu Penfluridol targets acid sphingomyelinase to inhibit TNF signaling and is therapeutic against inflammatory autoimmune diseases Arthritis Research & Therapy, 2022;24(1):27. 2022 [PMID: 35045889] (Bioassay, Mouse) Bioassay Mouse
Y Alwarawrah, K Danzaki, AG Nichols, BE Fee, C Bock, G Kucera, LP Hale, GA Taylor, NJ MacIver Irgm1 regulates metabolism and function in T cell subsets Scientific Reports, 2022;12(1):850. 2022 [PMID: 35039539] (Bioassay, Mouse) Bioassay Mouse
V Patel, A Jayaraman, S Jayaraman Epigenetic drug ameliorated type 1 diabetes via decreased generation of Th1 and Th17 subsets and restoration of self-tolerance in CD4+ T cells International immunopharmacology, 2021;103(0):108490. 2021 [PMID: 34954557] (Bioassay, Mouse) Bioassay Mouse
M Gurusamy, D Tischner, J Shao, S Klatt, S Zukunft, R Bonnavion, S Günther, K Siebenbrod, RI Kestner, T Kuhlmann, I Fleming, S Offermanns, N Wettschure G-protein-coupled receptor P2Y10 facilitates chemokine-induced CD4 T cell migration through autocrine/paracrine mediators Nature Communications, 2021;12(1):6798. 2021 [PMID: 34815397] (Bioassay, Mouse) Bioassay Mouse
MI Matias, CS Yong, A Foroushani, C Goldsmith, C Mongellaz, E Sezgin, KR Levental, A Talebi, J Perrault, A Rivière, J Dehairs, O Delos, J Bertand-Mi, JC Portais, M Wong, JC Marie, A Kelekar, S Kinet, VS Zimmermann, I Levental, L Yvan-Charv, JV Swinnen, SA Muljo, H Hernandez-, S Tardito, N Taylor, V Dardalhon Regulatory T�cell differentiation is controlled by alphaKG-induced alterations in mitochondrial metabolism and lipid homeostasis Cell Reports, 2021;37(5):109911. 2021 [PMID: 34731632] (Bioassay, Human) Bioassay Human
L Dai, Q Wang, X Lv, F Gao, Z Chen, Y Shen Elevated beta-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer&#039;s disease Brain, Behavior, and Immunity, 2021;98(0):337-348. 2021 [PMID: 34500034] (Bioassay, Mouse) Bioassay Mouse
A Osman, B Yan, Y Li, KD Pavelko, J Quandt, A Saadalla, MP Singh, M Kazemian, F Gounari, K Khazaie TCF-1 controls Treg cell functions that regulate inflammation, CD8+ T cell cytotoxicity and severity of colon cancer Nature Immunology, 2021;0(0):. 2021 [PMID: 34385712] (Bioassay, Mouse) Bioassay Mouse
J Zhou, X Zhang, J Hu, R Qu, Z Yu, H Xu, H Chen, L Yan, C Ding, Q Zou, Y Ye, Z Wang, RA Flavell, HB Li m6A demethylase ALKBH5 controls CD4+ T cell pathogenicity and promotes autoimmunity Science Advances, 2021;7(25):. 2021 [PMID: 34134995] (Bioassay, Mouse) Bioassay Mouse
S Sheppard, EK Santosa, CM Lau, S Violante, P Giovanelli, H Kim, JR Cross, MO Li, JC Sun Lactate dehydrogenase A-dependent aerobic glycolysis promotes natural killer cell anti-viral and anti-tumor function Cell Reports, 2021;35(9):109210. 2021 [PMID: 34077737] (Bioassay, Mouse) Bioassay Mouse
D Guan, Z Wang, J Huo, S Xu, KP Lam Bruton&#039;s tyrosine kinase regulates gut immune homeostasis through attenuating Th1 response Cell Death & Disease, 2021;12(5):431. 2021 [PMID: 33931590] (Bioassay, Mouse) Bioassay Mouse
Show All 237 Publications.

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