Recombinant Mouse IL-12 Protein, CF

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Recombinant Mouse IL-12 (Catalog # 419‑ML/CF) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01-0.1 ng/mL.
1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 25 kDa and 43 kDa (R) and 68 kDa (NR) .

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-12 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
(Met23-Ser335)
Accession # P43432
Mouse IL-12 p35
(Arg23-Ala215)
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23 (p40) & Arg23 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12a
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
SDS-PAGE
70 kDa, non-reducing conditions
Publications
Read Publications using
419-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein, CF

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems’ recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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Publications for IL-12 (419-ML/CF)(257)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(213)
Cell Culture
(11)
ELISA (Standard)
(8)
In Vivo
(26)
All Applications
Filter By Species
Human
(3)
Mouse
(245)
Rat
(2)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 257. Show All 257 Publications.
Publications using 419-ML/CF Applications Species
KA Read, DM Jones, S Pokhrel, EDS Hales, A Varkey, JA Tuazon, CD Eisele, O Abdouni, A Saadey, MR Leonard, RT Warren, MD Powell, JM Boss, EA Hemann, JS Yount, G Xin, HE Ghoneim, CJ Lio, AG Freud, PL Collins, KJ Oestreich Aiolos represses CD4+ T cell cytotoxic programming via reciprocal regulation of TFH transcription factors and IL-2 sensitivity Nature Communications, 2023-03-24;14(1):1652. 2023-03-24 [PMID: 36964178] (Bioassay, Mouse) Bioassay Mouse
Y Liao, L Sun, M Nie, J Li, X Huang, S Heng, W Zhang, T Xia, Z Guo, Q Zhao, LJ Zhang Modulation of Skin Inflammatory Responses by Aluminum Adjuvant Pharmaceutics, 2023-02-08;15(2):. 2023-02-08 [PMID: 36839900] (Bioassay, Mouse) Bioassay Mouse
WL Yang, W Qiu, T Zhang, K Xu, ZJ Gu, Y Zhou, HJ Xu, ZZ Yang, B Shen, YL Zhao, Q Zhou, Y Yang, W Li, PY Yang, YG Yang Nsun2 coupling with RoRgammat shapes the fate of Th17 cells and promotes colitis Nature Communications, 2023-02-16;14(1):863. 2023-02-16 [PMID: 36792629] (Bioassay, Mouse) Bioassay Mouse
X Hu, Y Zou, DA Copland, LP Schewitz-B, Y Li, PJP Lait, M Stimpson, Z Zhang, S Guo, J Liang, T Chen, JJ Li, S Yuan, S Li, P Zhou, Y Liu, AD Dick, X Wen, RWJ Lee, L Wei Epigenetic drug screen identified IOX1 as an inhibitor of Th17-mediated inflammation through targeting TET2 EBioMedicine, 2022-11-03;86(0):104333. 2022-11-03 [PMID: 36335665] (Bioassay, Mouse, Transgenic Mouse) Bioassay Mouse, Transgenic Mouse
SA Park, YJ Lim, WL Ku, D Zhang, K Cui, LY Tang, C Chia, P Zanvit, Z Chen, W Jin, D Wang, J Xu, O Liu, F Wang, A Cain, N Guo, H Nakatsukas, C Wu, YE Zhang, K Zhao, W Chen Opposing functions of circadian protein DBP and atypical E2F family E2F8 in anti-tumor Th9 cell differentiation Nature Communications, 2022-10-14;13(1):6069. 2022-10-14 [PMID: 36241625] (Bioassay, Mouse) Bioassay Mouse
M Hashimoto, K Araki, MA Cardenas, P Li, RR Jadhav, HT Kissick, WH Hudson, DJ McGuire, RC Obeng, A Wieland, J Lee, DT McManus, JL Ross, SJ Im, J Lee, JX Lin, B Hu, EE West, CD Scharer, GJ Freeman, AH Sharpe, SS Ramalingam, A Pellerin, V Teichgräbe, WJ Greenleaf, C Klein, JJ Goronzy, P Umaña, WJ Leonard, KA Smith, R Ahmed PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program Nature, 2022-09-28;610(7930):173-181. 2022-09-28 [PMID: 36171288] (Bioassay, Mouse) Bioassay Mouse
J Zhong, X Yang, J Chen, K He, X Gao, X Wu, M Zhang, H Zhou, F Xiao, L An, X Wang, Y Shi, N Zhang Circular EZH2-encoded EZH2-92aa mediates immune evasion in glioblastoma via inhibition of surface NKG2D ligands Nature Communications, 2022-08-15;13(1):4795. 2022-08-15 [PMID: 35970825] (Bioassay, Mouse) Bioassay Mouse
Y Su, X Sun, X Liu, Q Qu, L Yang, Q Chen, F Liu, Y Li, Q Wang, B Huang, XJ Huang, XH Zhang hUC-EVs-ATO reduce the severity of acute GVHD by resetting inflammatory macrophages toward the M2 phenotype Journal of hematology & oncology, 2022-07-21;15(1):99. 2022-07-21 [PMID: 35864538] (Bioassay, Mouse) Bioassay Mouse
A Merlini, M Haberl, J Strau beta , L Hildebrand, N Genc, J Franz, D Chilov, K Alitalo, C Flügel-Koc, C Stadelmann, A Flügel, F Odoardi Distinct roles of the meningeal layers in CNS autoimmunity Nature Neuroscience, 2022-06-30;0(0):. 2022-06-30 [PMID: 35773544] (Bioassay, Mouse) Bioassay Mouse
HZ Yu, BQ Zhu, L Zhu, S Li, LM Wang NR4A1 agonist cytosporone B attenuates neuroinflammation in a mouse model of multiple sclerosis Oncogene, 2022-12-01;17(12):2765-2770. 2022-12-01 [PMID: 35662227] (Bioassay, Mouse) Bioassay Mouse
Show All 257 Publications.

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