Recombinant Mouse IL-12 Protein, CF

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1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).
Recombinant Mouse IL-12 (Catalog # 419‑ML/CF) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01-0.1 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Mouse IL-12 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
(Met23-Ser335)
Accession # P43432
Mouse IL-12 p35
(Arg23-Ala215)
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23 (p40) & Arg23 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12a
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

SDS-PAGE
70 kDa, non-reducing conditions
Publications
Read Publications using
419-ML/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein, CF

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems’ recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

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419-ML/CF
Species: Mu
Applications: Bioactivity

Publications for IL-12 (419-ML/CF)(190)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(152)
Cell Culture
(3)
ELISA (Standard)
(8)
In Vivo
(23)
All Applications
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Human
(1)
Mouse
(179)
Rat
(2)
N/A
(6)
Transgenic Mouse
(1)
All Species
Showing Publications 1 - 10 of 190. Show All 190 Publications.
Publications using 419-ML/CF Applications Species
C Morales De, JR Maxwell, MM Xu, A Menoret, P Mittal, N Tsurutani, AJ Adler, AT Vella Costimulation Induces CD4�T Cell Antitumor Immunity via an Innate-like Mechanism Cell Rep, 2019;27(5):1434-1445.e3. 2019 [PMID: 31042471] (Bioassay, Mouse) Bioassay Mouse
TA Mace, MB Ware, SA King, S Loftus, MR Farren, E McMichael, S Scoville, C Geraghty, G Young, WE Carson, SK Clinton, GB Lesinski Soy isoflavones and their metabolites modulate cytokine-induced natural killer cell function Sci Rep, 2019;9(1):5068. 2019 [PMID: 30911044] (In Vivo, Mouse) In Vivo Mouse
P Kumar, K Rajasekara, A Nanbakhsh, J Gorski, MS Thakar, S Malarkanna IL-27 promotes NK cell effector functions via Maf-Nrf2 pathway during influenza infection Sci Rep, 2019;9(1):4984. 2019 [PMID: 30899058] (Bioassay, Mouse) Bioassay Mouse
Y Liu, Y Song, D Lin, L Lei, Y Mei, Z Jin, H Gong, Y Zhu, B Hu, Y Zhang, L Zhao, HY Teo, J Qiu, W Jiang, C Dong, D Wu, Y Huang, H Liu NCR- group 3 innate lymphoid cells orchestrate IL-23/IL-17 axis to promote hepatocellular carcinoma development EBioMedicine, 2019;0(0):. 2019 [PMID: 30827928] (Cell Culture, Mouse) Cell Culture Mouse
M Ott, E Avendaño-G, E Ullrich, C Dreyer, J Strauss, M Harden, M Schön, MP Schön, G Bernhardt, C Stadelmann, C Wegner, W Brück, S Nessler Laquinimod, a prototypic quinoline-3-carboxamide and aryl hydrocarbon receptor agonist, utilizes a CD155-mediated natural killer/dendritic cell interaction to suppress CNS autoimmunity J Neuroinflammation, 2019;16(1):49. 2019 [PMID: 30808363] (Bioassay, Mouse) Bioassay Mouse
KL Graham, BJ Werner, KM Moyer, AK Patton, CR Krois, HS Yoo, M Tverskoy, M LaJevic, JL Napoli, RA Sobel, BA Zabel, EC Butcher DGAT1 inhibits retinol-dependent regulatory T cell formation and mediates autoimmune encephalomyelitis Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 30718413] (Bioassay, Mouse) Bioassay Mouse
N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity J. Immunol., 2019;0(0):. 2019 [PMID: 30718299] (Bioassay, Mouse) Bioassay Mouse
K Yasuda, Y Kitagawa, R Kawakami, Y Isaka, H Watanabe, G Kondoh, T Kohwi-Shig, S Sakaguchi, K Hirota Satb1 regulates the effector program of encephalitogenic tissue Th17 cells in chronic inflammation Nat Commun, 2019;10(1):549. 2019 [PMID: 30710091] (Bioassay, Mouse) Bioassay Mouse
TS Abraham, JC Flickinger, SA Waldman, AE Snook TCR Retrogenic Mice as a Model To Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen GUCY2C J. Immunol., 2019;0(0):. 2019 [PMID: 30642983] (Bioassay, Mouse) Bioassay Mouse
M Amir, S Chaudhari, R Wang, S Campbell, SA Mosure, LB Chopp, Q Lu, J Shang, OB Pelletier, Y He, C Doebelin, MD Cameron, DJ Kojetin, TM Kamenecka, LA Solt REV-ERB? Regulates TH17 Cell Development and Autoimmunity Cell Rep, 2018;25(13):3733-3749.e8. 2018 [PMID: 30590045] (Bioassay, Mouse) Bioassay Mouse
Show All 190 Publications.

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