Recombinant Mouse IL-12 Protein, CF


1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).
Recombinant Mouse IL-12 (Catalog # 419‑ML/CF) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01-0.1 ng/mL.

Product Details

Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Mouse IL-12 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
Accession # P43432
Mouse IL-12 p35
Accession # NP_032377
N-terminus C-terminus
Accession #
N-terminal Sequence
Met23 (p40) & Arg23 (p35)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


70 kDa, non-reducing conditions
Read Publications using
419-ML/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12 Protein, CF

  • CLMF p35
  • CLMF1
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12 subunit p35
  • IL12
  • IL-12
  • IL-12, subunit p35
  • IL12A
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • natural killer cell stimulatory factor 1, 35 kD subunit
  • NF cell stimulatory factor chain 1
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35


Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems’ recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 - G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, ICC
Species: Mu
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu
Applications: WB, Simple Western, IHC, IP
Species: Hu, Rt
Applications: WB, IHC, IHC-P
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, KO
Species: Mu
Applications: Bioactivity

Publications for IL-12 (419-ML/CF)(190)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, N/A, Transgenic Mouse.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo.

Filter By Application
Cell Culture
ELISA (Standard)
In Vivo
All Applications
Filter By Species
Transgenic Mouse
All Species
Showing Publications 1 - 10 of 190. Show All 190 Publications.
Publications using 419-ML/CF Applications Species
C Morales De, JR Maxwell, MM Xu, A Menoret, P Mittal, N Tsurutani, AJ Adler, AT Vella Costimulation Induces CD4�T Cell Antitumor Immunity via an Innate-like Mechanism Cell Rep, 2019;27(5):1434-1445.e3. 2019 [PMID: 31042471] (Bioassay, Mouse) Bioassay Mouse
TA Mace, MB Ware, SA King, S Loftus, MR Farren, E McMichael, S Scoville, C Geraghty, G Young, WE Carson, SK Clinton, GB Lesinski Soy isoflavones and their metabolites modulate cytokine-induced natural killer cell function Sci Rep, 2019;9(1):5068. 2019 [PMID: 30911044] (In Vivo, Mouse) In Vivo Mouse
P Kumar, K Rajasekara, A Nanbakhsh, J Gorski, MS Thakar, S Malarkanna IL-27 promotes NK cell effector functions via Maf-Nrf2 pathway during influenza infection Sci Rep, 2019;9(1):4984. 2019 [PMID: 30899058] (Bioassay, Mouse) Bioassay Mouse
Y Liu, Y Song, D Lin, L Lei, Y Mei, Z Jin, H Gong, Y Zhu, B Hu, Y Zhang, L Zhao, HY Teo, J Qiu, W Jiang, C Dong, D Wu, Y Huang, H Liu NCR- group 3 innate lymphoid cells orchestrate IL-23/IL-17 axis to promote hepatocellular carcinoma development EBioMedicine, 2019;0(0):. 2019 [PMID: 30827928] (Cell Culture, Mouse) Cell Culture Mouse
M Ott, E Avendaño-G, E Ullrich, C Dreyer, J Strauss, M Harden, M Schön, MP Schön, G Bernhardt, C Stadelmann, C Wegner, W Brück, S Nessler Laquinimod, a prototypic quinoline-3-carboxamide and aryl hydrocarbon receptor agonist, utilizes a CD155-mediated natural killer/dendritic cell interaction to suppress CNS autoimmunity J Neuroinflammation, 2019;16(1):49. 2019 [PMID: 30808363] (Bioassay, Mouse) Bioassay Mouse
KL Graham, BJ Werner, KM Moyer, AK Patton, CR Krois, HS Yoo, M Tverskoy, M LaJevic, JL Napoli, RA Sobel, BA Zabel, EC Butcher DGAT1 inhibits retinol-dependent regulatory T cell formation and mediates autoimmune encephalomyelitis Proc. Natl. Acad. Sci. U.S.A., 2019;0(0):. 2019 [PMID: 30718413] (Bioassay, Mouse) Bioassay Mouse
N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity J. Immunol., 2019;0(0):. 2019 [PMID: 30718299] (Bioassay, Mouse) Bioassay Mouse
K Yasuda, Y Kitagawa, R Kawakami, Y Isaka, H Watanabe, G Kondoh, T Kohwi-Shig, S Sakaguchi, K Hirota Satb1 regulates the effector program of encephalitogenic tissue Th17 cells in chronic inflammation Nat Commun, 2019;10(1):549. 2019 [PMID: 30710091] (Bioassay, Mouse) Bioassay Mouse
TS Abraham, JC Flickinger, SA Waldman, AE Snook TCR Retrogenic Mice as a Model To Map Self-Tolerance Mechanisms to the Cancer Mucosa Antigen GUCY2C J. Immunol., 2019;0(0):. 2019 [PMID: 30642983] (Bioassay, Mouse) Bioassay Mouse
M Amir, S Chaudhari, R Wang, S Campbell, SA Mosure, LB Chopp, Q Lu, J Shang, OB Pelletier, Y He, C Doebelin, MD Cameron, DJ Kojetin, TM Kamenecka, LA Solt REV-ERB? Regulates TH17 Cell Development and Autoimmunity Cell Rep, 2018;25(13):3733-3749.e8. 2018 [PMID: 30590045] (Bioassay, Mouse) Bioassay Mouse
Show All 190 Publications.

Reviews for IL-12 (419-ML/CF) (0)

There are no reviews for IL-12 (419-ML/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-12 (419-ML/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-12 Products

Bioinformatics Tool for IL-12 (419-ML/CF)

Discover related pathways, diseases and genes to IL-12 (419-ML/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-12 (419-ML/CF)

Discover more about diseases related to IL-12 (419-ML/CF).

Pathways for IL-12 (419-ML/CF)

View related products by pathway.

PTMs for IL-12 (419-ML/CF)

Learn more about PTMs related to IL-12 (419-ML/CF).

Blogs on IL-12.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Ly6G - a marker for monocytes, granulocytes and neutrophils
Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes expres...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse IL-12 Protein, CF and receive a gift card or discount.