IL-12 Antibodies

219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." class="big_lightbox"> 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." alt="Recombinant Human IL-12 (219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA). The ND₅₀ is typically 5-50 ng/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm . View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm . View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 309-IL) was serially diluted and captured by Mouse Anti-Human IL‑12/IL‑23 p40 Monoclonal Antibody (Catalog # MAB609) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

219-IL) was serially diluted and captured by Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 219-IL) was serially diluted and captured by Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-219-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_lightbox"> 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." alt="Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-419-NA). The ND₅₀ is typically 0.01-0.06 µg/mL." class="big_thumb" />

219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND₅₀ is typically 1-2 µg/mL." class="big_lightbox"> 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND₅₀ is typically 1-2 µg/mL." alt="Recombinant Human IL‑12 (Catalog # 219-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL‑12 Polyclonal Antibody (Catalog # AB-219-NA). The ND₅₀ is typically 1-2 µg/mL." class="big_thumb" />

419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-12 Polyclonal Antibody (Catalog # AB-419-NA). The ND₅₀ is typically 0.2-0.5 µg/mL." class="big_lightbox"> 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-12 Polyclonal Antibody (Catalog # AB-419-NA). The ND₅₀ is typically 0.2-0.5 µg/mL." alt="Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-12 (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-12 Polyclonal Antibody (Catalog # AB-419-NA). The ND₅₀ is typically 0.2-0.5 µg/mL." class="big_thumb" />

1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_lightbox"> 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND₅₀ is typically 0.05-0.25 µg/mL." alt="Recombinant Rat IL-12 (Catalog # 1760-RL) stimulates proliferation in mouse splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1760). The ND₅₀ is typically 0.05-0.25 µg/mL." class="big_thumb" />

912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND₅₀ is typically 0.01-0.04 µg/mL." class="big_lightbox"> 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND₅₀ is typically 0.01-0.04 µg/mL." alt="Recombinant Porcine IL‑12 (Catalog # 912-PL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL‑12 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine IL‑12 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF912). The ND₅₀ is typically 0.01-0.04 µg/mL." class="big_thumb" />

F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC monocytes treated with IFN-gamma (Catalog # 285-IF, 10 ng/mL for 2 hours), then LPS (100 ng/mL for 12 hours), and lastly monensin (3 µM for 3 hours) (A) vs naïve PBMC monocytes (B) were stained with Rat Anti-Human IL‑12 Monoclonal Antibody (Catalog # MAB11485) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113) and subsequently Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />