Ly6G - a marker for monocytes, granulocytes and neutrophils

Thu, 11/20/2014 - 09:22

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a 21-25kD glycosylphosphatidylinositol (GPI)-linked differentiation antigen that is expressed by myeloid-derived cells in a tightly developmentally-regulated manner in the bone marrow. Monocytes express Ly6G transiently during bone marrow development, while Ly6G expression in granulocytes and peripheral neutrophils directly correlates with the cell’s level of differentiation and maturation. This hallmark makes Ly6G a good marker for these particular cell populations. Ly6G has also been implicated in the development of antitumor responses. 

ly-6g antibody

Gr-1/Ly-6G Antibody (RB6-8C5) [MAB1037]
Ly-6G/Ly-6C (Gr-1) was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1)Monoclonal Antibody (Catalog # MAB1037) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Researchers from the Genetics Institute at Andover used the Ly6G antibody to demonstrate that systemic administration of recombinant murine IL-12, as delivered by modified acute myeloid leukemia (AML) cells, generates a potent and long-lasting protective immunity in AML patients1. Miura et al relied upon the Ly6G antibody to test the effects of antibodies to KC/Gro alpha and MIP-2 on blocking neutrophil infiltration into kidneys after injury2. Their studies found that neutralizing such chemokines and minimizing resultant pathology and damage. Alvarez’s group at the used the Ly6G antibody to map secreted protein acidic and rich in cysteine (SPARC) downstream events in human melanoma cells3. Another group found that Toll-like receptor signaling is crucial for Listeria monocytogenes (Lm) bacterial-triggered tumor growth through infection-associated pathways4. Their novel work sheds light upon the importance of antibiotic treatment therapy in conjunction with more conventional oncology therapies. Additionally, the Ly6G antibody allowed researchers to examine the role of IL-27 in modulating collagen-induced arthritis5. Their rheumatology experiments focused on understanding the MOA of IL-27 and IL-17 and their downstream modulators, with hopes of treating arthritis patients. Novus Biologicals offers Ly6G reagents for your research needs including:


  1. 10590071
  2. 11733364
  3. 15958556
  4. 17483348
  5. 21384333

Blog Topics