Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 65-95% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-1/CD54 protein
Mouse ICAM-1
(Gln28 - Asn485)
Accession # Q3U8M7
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln28
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Icam1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
76.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-130 kDa, reducing conditions
Publications
Read Publications using
796-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 0.4 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

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Publications for ICAM-1/CD54 (796-IC)(52)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Bioassay, Cell Adhesion, Ctrl, Flow, Surface Plasmon Resonance.


Filter By Application
Bioassay
(48)
Cell Adhesion
(1)
Ctrl
(1)
Flow
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(48)
N/A
(2)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using 796-IC Applications Species
A Farinazzo, S Angiari, E Turano, E Bistaffa, S Dusi, S Ruggieri, R Bonafede, R Mariotti, G Constantin, B Bonetti Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis Sci Rep, 2018;8(1):7473. 2018 [PMID: 29748664] (Bioassay, Mouse) Bioassay Mouse
L Ju, Y Chen, K Li, Z Yuan, B Liu, SP Jackson, C Zhu Dual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species Sci Rep, 2017;7(1):14185. 2017 [PMID: 29079742] (Bioassay, Mouse) Bioassay Mouse
CP Basole, RK Nguyen, K Lamothe, A Vang, R Clark, GS Baillie, PM Epstein, S Brocke PDE8 controls CD4(+) T cell motility through the PDE8A-Raf-1 kinase signaling complex Cell. Signal., 2017;40(0):62-72. 2017 [PMID: 28851628] (Bioassay, Mouse) Bioassay Mouse
JG Borger, VL Morrison, A Filby, C Garcia, LM Uotila, F Simbari, SC Fagerholm, R Zamoyska Caveolin-1 Influences LFA-1 Redistribution upon TCR Stimulation in CD8 T Cells J. Immunol., 2017;0(0):. 2017 [PMID: 28637901] (Bioassay, Mouse) Bioassay Mouse
BR Gittens, JV Bodkin, S Nourshargh, M Perretti, D Cooper Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation J. Immunol., 2017;0(0):. 2017 [PMID: 28438899] (Bioassay, Mouse) Bioassay Mouse
Y Hirano, WL Yang, M Aziz, F Zhang, B Sherry, P Wang MFG-E8-derived peptide attenuates adhesion and migration of immune cells to endothelial cells J. Leukoc. Biol, 2017;0(0):. 2017 [PMID: 28096298] (Bioassay, Mouse) Bioassay Mouse
Sophia Koutsogian Prolonged exposure to volatile anesthetic isoflurane worsens the outcome of polymicrobial abdominal sepsis Toxicol. Sci, 2016;0(0):. 2016 [PMID: 28003439] (Bioassay, Mouse) Bioassay Mouse
Janice Chou Glycogen storage disease type Ib neutrophils exhibit impaired cell adhesion and migration Biochem. Biophys. Res. Commun., 2016;0(0):. 2016 [PMID: 27864142] (Bioassay, Mouse) Bioassay Mouse
Aged neutrophils contribute to the first line of defense in the acute inflammatory response Blood, 2016;0(0):. 2016 [PMID: 27609642] (Flow, Mouse) Flow Mouse
ADAP plays a pivotal role in CD4+ T cell activation but is only marginally involved in CD8+ T cell activation, differentiation, and immunity to pathogens J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27605210] (Bioassay, Mouse) Bioassay Mouse
Show All 52 Publications.

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Bioinformatics

Gene Symbol Icam1
Entrez
Uniprot