Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 104 cells/well are added to rmICAM/Fc Chimera coated plates (12.5 µg/mL with 100 µL/well), approximately 65-95% will adhere after 1 hour incubation at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived mouse ICAM-1/CD54 protein
Mouse ICAM-1
(Gln28-Asn485)
Accession # Q3U8M7
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gln28
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Icam1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
76.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
110-130 kDa, reducing conditions
Publications
Read Publications using
796-IC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 0.4 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF

  • BB2
  • CD54 antigen
  • CD54
  • cell surface glycoprotein P3.58
  • human rhinovirus receptor
  • ICAM1
  • ICAM-1
  • intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
  • intercellular adhesion molecule 1
  • Major group rhinovirus receptor
  • P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

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796-IC
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Applications: Bioactivity

Publications for ICAM-1/CD54 (796-IC)(65)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 7 applications: Adhesion Assay, Binding Assay, Bioassay, Cell Culture, Control, Flow Cytometry, Surface Plasmon Resonance.


Filter By Application
Adhesion Assay
(1)
Binding Assay
(1)
Bioassay
(58)
Cell Culture
(1)
Control
(1)
Flow Cytometry
(2)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(61)
N/A
(2)
All Species
Showing Publications 1 - 10 of 65. Show All 65 Publications.
Publications using 796-IC Applications Species
NH Roy, M Mammadli, JK Burkhardt, M Karimi CrkL is required for donor T cell migration to GvHD target organs Oncotarget, 2020;11(17):1505-1514. 2020 [PMID: 32391120] (Bioassay, Mouse) Bioassay Mouse
A Salz, C Gurniak, F Jönsson, W Witke Cofilin1 driven actin dynamics controls migration of thymocytes and is essential for positive selection in the thymus J. Cell. Sci., 2020;0(0):. 2020 [PMID: 31974112] (Bioassay, Mouse) Bioassay Mouse
C Ren, Q Yuan, X Jian, PA Randazzo, W Tang, D Wu Small GTPase ARF6 Is a Coincidence-Detection Code for RPH3A Polarization in Neutrophil Polarization J. Immunol., 2020;0(0):. 2020 [PMID: 31924649] (Bioassay, Mouse) Bioassay Mouse
GS Gülcüler B, C Monzel, S Kleber, J Beaudouin, E Balta, T Kaindl, S Chen, L Gao, M Thiemann, CR Wirtz, Y Samstag, M Tanaka, A Martin-Vil 3D Cellular Architecture Modulates Tyrosine Kinase Activity, Thereby Switching CD95-Mediated Apoptosis to Survival Cell Rep, 2019;29(8):2295-2306.e6. 2019 [PMID: 31747602] (Binding Assay, Mouse) Binding Assay Mouse
D Engelberts, A Autio, RAF Verwillige, MAC Depuydt, G Newton, S Rattik, E Levinsohn, G Saggu, P Jarolim, H Wang, F Velazquez, AH Lichtman, FW Luscinskas Increased lymphocyte activation and atherosclerosis in CD47-deficient mice Sci Rep, 2019;9(1):10608. 2019 [PMID: 31337788] (Cell Culture, Mouse) Cell Culture Mouse
MC Schmid, SQ Khan, MM Kaneda, P Pathria, R Shepard, TL Louis, S Anand, G Woo, C Leem, MH Faridi, T Geraghty, A Rajagopala, S Gupta, M Ahmed, RI Vazquez-Pa, DA Cheresh, V Gupta, JA Varner Integrin CD11b activation drives anti-tumor innate immunity Nat Commun, 2018;9(1):5379. 2018 [PMID: 30568188] (Bioassay, Mouse) Bioassay Mouse
J Chen, N Li, Y Yin, N Zheng, M Min, B Lin, L Zhang, X Long, Y Zhang, Z Cai, S Zhai, J Qin, X Wang Methyltransferase Nsd2 Ensures Germinal Center Selection by Promoting Adhesive Interactions between B Cells and Follicular Dendritic Cells Cell Rep, 2018;25(12):3393-3404.e6. 2018 [PMID: 30566865] (Bioassay, Mouse) Bioassay Mouse
RJ Kimmerling, SM Prakadan, AJ Gupta, NL Calistri, MM Stevens, S Olcum, N Cermak, RS Drake, K Pelton, F De Smet, KL Ligon, AK Shalek, SR Manalis Linking single-cell measurements of mass, growth rate, and gene expression Genome Biol., 2018;19(1):207. 2018 [PMID: 30482222] (Bioassay, Mouse) Bioassay Mouse
FA Moretti, S Klapproth, R Ruppert, A Margraf, J Weber, R Pick, C Scheierman, M Sperandio, R Fässler, M Moser Differential requirement of kindlin-3 for T cell progenitor homing to the non-vascularized and vascularized thymus Elife, 2018;7(0):. 2018 [PMID: 30187863] (Bioassay, Mouse) Bioassay Mouse
M Keszei, J Record, JS Kritikou, H Wurzer, C Geyer, M Thiemann, P Drescher, H Brauner, L Köcher, J James, M He, MA Baptista, CI Dahlberg, A Biswas, S Lain, DP Lane, W Song, K Pütsep, P Vandenberg, SB Snapper, LS Westerberg Constitutive activation of WASp in X-linked neutropenia renders neutrophils hyperactive J. Clin. Invest., 2018;128(9):4115-4131. 2018 [PMID: 30124469] (Bioassay, Mouse) Bioassay Mouse
Show All 65 Publications.

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Bioinformatics

Gene Symbol Icam1
Uniprot