Reactivity | MuSpecies Glossary |
Applications | Bioactivity |
Details of Functionality | Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2 μg/mL in the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB). |
Source | Mouse myeloma cell line, NS0-derived mouse FGF-21 protein Tyr30-Ser210 |
Accession # | |
N-terminal Sequence | Tyr30 & Tyr49 |
Protein/Peptide Type | Recombinant Proteins |
Gene | Fgf21 |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note | <0.10 EU per 1 μg of the protein by the LAL method. |
Dilutions |
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Theoretical MW | 20 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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SDS-PAGE | 22-25 kDa, reducing conditions |
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Publications |
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Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Buffer | Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein. |
Purity | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions | Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin. |
Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. Based on its structure, FGF-21 is further classified into a subfamily of FGFs along with FGF-19 and -23 (1). Mouse FGF-21 is a 210 amino acid (aa) polypeptide that contains a 120 aa core FGF domain and a hydrophobic N-terminus signal sequence. The signal sequence is cleaved to release the soluble 180 aa mature FGF-21 protein (2). At the amino acid sequence level, mature mouse FGF-21 is 81% and 92% identical to mature human and rat FGF-21, respectively. In comparison to other FGF subfamilies, a heparin-binding domain is uniquely absent in FGF-19 subfamily members. Lack of this domain confers endocrine function to FGF-19 members and enables them to freely diffuse within tissues and accumulate in the circulatory system (3, 4). The biological activity of FGF-21 requires binding to Klotho beta , a co-receptor that is in complex with cell surface FGF receptors (FGF R) (5, 6). Binding of FGF-21 to Klotho beta facilitates FGF R activation and autophosphorylation resulting in the initiation of multiple downstream signaling cascades (7, 8). FGF-21 cannot independently bind to FGF Rs, thus its effects are restricted to target tissues that express Klotho beta . FGF-21 functions as a physiological regulator of cellular metabolism, including glucose uptake in adipocytes and cellular sensitivity to insulin. FGF-21 is basally expressed in the pancreas, thymus, and liver, as well as in adipose tissue (3, 9). Local and systemic metabolic stress has been shown to induce expression of FGF-21 in the liver, muscles, and fat (10-12). Modulation of FGF-21 expression is associated with a number of metabolic disorders, including obesity and diabetes (7, 13). FGF-21 is also involved in promoting cell survival and proliferation, modulating mesenchymal stem cell differentiation, regulating circadian rhythm, and controlling reproductive capacity during nutrient deprivation (14-18).
PTMs for FGF-21 (8409-FG)Learn more about PTMs related to FGF-21 (8409-FG).
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