Recombinant Mouse FGF-21 Protein

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Recombinant Mouse FGF-21 stimulated proliferation in NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4‑2 μg/mL in the presence of Recombinant Mouse Klotho beta (Catalog # ...read more

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Mouse FGF-21 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2 μg/mL in the presence of Recombinant Mouse Klotho  beta (Catalog # 2619-KB).
Source
Mouse myeloma cell line, NS0-derived mouse FGF-21 protein
Tyr30-Ser210
Accession #
N-terminal Sequence
Tyr30 & Tyr49
Protein/Peptide Type
Recombinant Proteins
Gene
Fgf21
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22-25 kDa, reducing conditions
Publications
Read Publications using
8409-FG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and EDTA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGF-21 Protein

  • FGF21
  • FGF-21
  • fibroblast growth factor 21

Background

Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family. Based on its structure, FGF-21 is further classified into a subfamily of FGFs along with FGF-19 and -23 (1). Mouse FGF-21 is a 210 amino acid (aa) polypeptide that contains a 120 aa core FGF domain and a hydrophobic N-terminus signal sequence. The signal sequence is cleaved to release the soluble 180 aa mature FGF-21 protein (2). At the amino acid sequence level, mature mouse FGF-21 is 81% and 92% identical to mature human and rat FGF-21, respectively. In comparison to other FGF subfamilies, a heparin-binding domain is uniquely absent in FGF-19 subfamily members. Lack of this domain confers endocrine function to FGF-19 members and enables them to freely diffuse within tissues and accumulate in the circulatory system (3, 4). The biological activity of FGF-21 requires binding to Klotho  beta , a co-receptor that is in complex with cell surface FGF receptors (FGF R) (5, 6). Binding of FGF-21 to Klotho  beta facilitates FGF R activation and autophosphorylation resulting in the initiation of multiple downstream signaling cascades (7, 8). FGF-21 cannot independently bind to FGF Rs, thus its effects are restricted to target tissues that express Klotho  beta . FGF-21 functions as a physiological regulator of cellular metabolism, including glucose uptake in adipocytes and cellular sensitivity to insulin. FGF-21 is basally expressed in the pancreas, thymus, and liver, as well as in adipose tissue (3, 9). Local and systemic metabolic stress has been shown to induce expression of FGF-21 in the liver, muscles, and fat (10-12). Modulation of FGF-21 expression is associated with a number of metabolic disorders, including obesity and diabetes (7, 13). FGF-21 is also involved in promoting cell survival and proliferation, modulating mesenchymal stem cell differentiation, regulating circadian rhythm, and controlling reproductive capacity during nutrient deprivation (14-18).

  1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
  2. Nishimura, T. et al. (2000) Biochim. Biophys. Acta 1492:203.
  3. Adams, A.C. et al. (2013) Mol. Metab. 2:205.
  4. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
  5. Suzuki, M. et al. (2008) Mol. Endocrinol. 22:1006.
  6. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  7. Gimeno, R.E. and D.E. Moller (2014) Trends Endocrinol. Metab. 25:303.
  8. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. U S A 104:7432.
  9. Kharitonenkov, A. et al. (2005) J. Clin. Invest. 115:1627.
  10. Luo, Y. and W.L. McKeehan (2013) Front. Endocrinol. (Lausanne) 4:194.
  11. Yang, C. et al. (2013) BMC Gastroenterol. 13:67.
  12. Keipert, S. et al. (2014) Am. J. Physiol. Endocrinol. Metab. 306:E469.
  13. Zhang, X. et al. (2008) Diabetes 57:1246.
  14. Wei, W. et al. (2012) Proc. Natl. Acad. Sci. USA 109:3143.
  15. Wei, W. et al. (2010) Cell. Metab. 11:503.
  16. Wan, Y. (2013) Int. J. Biochem. Cell. Biol. 45:546.
  17. Bookout, A.L. et al. (2013) Nat. Med. 19:1147.
  18. Owen, B.M. et al. (2013) Nat. Med. 19:1153.

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Publications for FGF-21 (8409-FG)(3)

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Bioinformatics

Gene Symbol Fgf21
Uniprot