Recombinant Mouse Dectin-1/CLEC7A Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Dectin-1/CLEC7A Protein, CF Summary

• Details of Functionality: Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated K_D <6 nM.
• Source: Mouse myeloma cell line, NS0-derived mouse Dectin-1/CLEC7A protein
  Phe69-Leu244, with an N-terminal 10-His tag
• Accession #: Q6QLQ4
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: Clec7a
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 21.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 27-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Dectin-1/CLEC7A Protein, CF

• Beta-glucan receptor
• BGR
• CD369
• CLEC7A
• CLECSF12
• CLECSF12DC-associated C-type lectin 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12
• C-type lectin domain family 7 member A
• C-type lectin domain family 7, member A
• C-type lectin superfamily member 12
• Dectin1
• Dectin-1
• DECTIN1CANDF4
• Dendritic cell-associated C-type lectin 1
• dendritic cell-associated C-type lectin-1
• hDectin-1
• lectin-like receptor 1

Background

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Publications for Dectin-1/CLEC7A (1756-DC)(3)

Publications using 1756-DC

• C Leclaire, K Lecointe, PA Gunning, S Tribolo, DW Kavanaugh, A Wittmann, D Latousakis, DA MacKenzie, N Kawasaki, N Juge Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins FASEB J., 2018-01-29;0(0):fj201700619R. 2018-01-29 [PMID: 29401627] (Bioassay, Mass Spectrometry, Mouse)
• AJ Smith, B Graves, R Child, PJ Rice, Z Ma, DW Lowman, HE Ensley, KT Ryter, JT Evans, DL Williams Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties J. Immunol., 2017-12-15;0(0):. 2017-12-15 [PMID: 29246954] (Bioassay)
• Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015-03-18;10(3):e0119244. 2015-03-18 [PMID: 25786219] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Clec7a
• Uniprot:
  • Q6QLQ4()