Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD117/c-kit Fc Chimera is present at 0.5 μg/mL, the concentration of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC) that produces 50% of the optimal binding response is approximately 3‑12 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CD117/c-kit protein
  

  Mouse CD117/c-kit (Gln26-Thr523) Accession # P05532	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: P05532
• N-terminal Sequence: Gln26 predicted: No results obtained, sequencing might be blocked
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Kit
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 82.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD117/c-kit Fc Chimera Protein, CF

• CD117 antigen
• CD117
• ckit
• c-kit
• EC 2.7.10
• EC 2.7.10.1
• KIT
• PBT
• piebald trait
• Proto-oncogene c-Kit
• proto-oncogene tyrosine-protein kinase Kit
• SCF R
• SCFR
• SCFRmast/stem cell growth factor receptor
• soluble KIT variant 1
• Tyrosine-protein kinase Kit
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
• v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

Background

Stem Cell Factor Receptor (SCF R), also known as c‑Kit and CD117, is a widely expressed 145 kDa receptor tyrosine kinase. It is the cellular homolog of the feline sarcoma virus protein, v‑Kit. Binding of SCF R to SCF, also known as Steel Factor and Kit Ligand, promotes the survival, differentiation, and mobilization of progenitor cells in multiple lineages (1‑4). Mutations or deletions of SCF R cause a wide variety of malignancies as well as pigmentation disorders and sterility (5, 6). Mature mouse SCF R consists of a 503 amino acid (aa) extracellular domain (ECD) with five tandem immunoglobulin‑like domains, a 21 aa transmembrane segment, and a 431 aa cytoplasmic domain with the split tyrosine kinase domain (7). Within the ECD, mouse SCF R shares 73% and 88% aa sequence identity with human and rat SCF R, respectively. Alternative splicing of mouse SCF R generates a truncated intracellular isoform that corresponds to the C‑terminal half of the tyrosine kinase domain (8). SCF is expressed as transmembrane and soluble noncovalent homodimers (9). One SCF dimer binds to two molecules of SCF R, inducing receptor dimerization and activation (9). Transmembrane SCF induces more prolonged signaling through SCF R compared to soluble SCF (10). Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (11). A 100 kDa glycosylated ECD fragment of SCF R can be shed into the circulation by TACE/ADAM17, and this fragment inhibits the interaction of SCF with transmembrane SCF R (12, 13). SCF is a primary growth and activation factor for mast cells and eosinophils (14). SCF R expression on mast cells enables them to infiltrate SCF‑secreting tumors where they promote tumor growth and induce local immune suppression (15). SCF R is up‑regulated on dendritic cells by Th2‑ or Th17‑biasing stimuli, and it is required for subsequent dendritic cell induction of Th2 and Th17 responses (16). SCF R protects vascular smooth muscle cells from apoptosis and assists in the recovery of cardiac function following myocardial infarction (17, 18).

1. Kimura, Y. et al. (2011) PLoS ONE 6:e26918.
2. Tallini, Y.N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:1808.
3. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
4. Sun, L. et al. (2004) J. Clin. Invest. 113:1364.
5. Pittoni, P. et al. (2011) Oncogene 30:757.
6. Sartini, S. et al. (2011) Curr. Med. Chem. 18:2893.
7. Qiu, F.H. et al. (1988) EMBO J. 7:1003.
8. Zayas, J. et al. (2008) Stem Cells Dev. 17:343.
9. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
10. Miyazawa, K. et al. (1995) Blood 85:641.
11. Martin, F.H. et al. (1990) Cell 63:203.
12. Turner, A.M. et al. (1995) Blood 85:2052.
13. Cruz, A.C. et al. (2004) J. Biol. Chem. 279:5612.
14. Jamur, M.C. and C. Oliver (2011) Front. Biosci. (School Ed.) 3:1390.
15. Huang, B. et al. (2008) Blood 112:1269.
16. Krishnamoorthy, N. et al. (2008) Nat. Med. 14:565.
17. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
18. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.

Publications for CD117/c-kit (1356-SR)(1)

Publication using 1356-SR

• M Schmitt, M Schewe, A Sacchetti, D Feijtel, WS van de Gee, M Teeuwssen, HF Sleddens, R Joosten, ME van Royen, HJG van de Wer, J van Es, H Clevers, R Fodde Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling Cell Rep, 2018;24(9):2312-2328.e7. 2018 [PMID: 30157426] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Kit
• Uniprot:
  • P05532()