Recombinant Human VEGF 165 Protein

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1 μg/lane of Recombinant Human VEGF165 was resolved by SDS-PAGE with silver staining, under reducing (R) and non-reducing (NR) conditions, showing major bands at 20-22 kDa and 39-42 kDa, respectively. Multiple bands in ...read more
Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human VEGF 165 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL.
The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein
Ala27-Arg191
Accession #
N-terminal Sequence
Ala27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
VEGFA
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-22 kDa, reducing conditions
39-42 kDa, non-reducing conditions
Publications
Read Publications using
293-VE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. Alternatively, reconstitute at 500 μg/mL in sterile 4 mM HCl containing 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF 165 Protein

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

  1. Leung, D.W. et al. (1989) Science 246:1306. 
  2. Keck, P.J. et al. (1989) Science 246:1309. 
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. 
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  6. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  7. Thurston, G. (2002) J. Anat. 200:575.
  8. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  9. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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293-VE
Species: Hu
Applications: Bioactivity

Publications for VEGF (293-VE)(303)

We have publications tested in 17 confirmed species: Human, Mouse, Rat, Avian - Quail, Bovine, Canine, Chicken, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Chlorocebus sabaeus (Green Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Transgenic Rabbit, Xenopus, Zebrafish.

We have publications tested in 14 applications: Array Development, Binding Assay, Bioassay, Cell Culture, ELISA, ELISA (Capture), ELISA (Standard), ELISA Development, Immunoassay Development, Immunoassay Standard, In Vivo, Quantum Dot, Surface Plasmon Resonance, Western Blot.


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Array Development
(1)
Binding Assay
(3)
Bioassay
(235)
Cell Culture
(2)
ELISA
(1)
ELISA (Capture)
(2)
ELISA (Standard)
(5)
ELISA Development
(1)
Immunoassay Development
(1)
Immunoassay Standard
(1)
In Vivo
(34)
Quantum Dot
(1)
Surface Plasmon Resonance
(3)
Western Blot
(4)
All Applications
Filter By Species
Human
(212)
Mouse
(32)
Rat
(10)
Avian - Quail
(1)
Bovine
(7)
Canine
(2)
Chicken
(3)
N/A
(10)
Porcine
(3)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(3)
Transgenic Rabbit
(1)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 303. Show All 303 Publications.
Publications using 293-VE Applications Species
LM Muellerlei, B Buxbaum, B Nell, DA Fux In-vitro binding analysis of anti-human vascular endothelial growth factor antibodies bevacizumab and aflibercept with canine, feline, and equine vascular endothelial growth factor Res. Vet. Sci., 2019;124(0):233-238. 2019 [PMID: 30933891] (ELISA, Human) ELISA Human
S Jauhiainen, JP Laakkonen, K Ketola, PI Toivanen, T Nieminen, T Ninchoji, AL Levonen, MU Kaikkonen, S Ylä-Herttu Axon Guidance-Related Factor FLRT3 Regulates VEGF-Signaling and Endothelial Cell Function Front Physiol, 2019;10(0):224. 2019 [PMID: 30930791] (Bioassay, Human) Bioassay Human
O Lyros, AK Lamprecht, L Nie, R Thieme, K Götzel, M Gasparri, G Haasler, P Rafiee, R Shaker, I Gockel Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett&#039;s associated esophageal adenocarcinoma Am J Cancer Res, 2019;9(2):330-346. 2019 [PMID: 30906632] (Bioassay, Human) Bioassay Human
T Emi, K Michaud, E Orton, G Santilli, C Linh, M O'Connell, F Issa, S Kennedy Ultrasonic Generation of Pulsatile and Sequential Therapeutic Delivery Profiles from Calcium-Crosslinked Alginate Hydrogels Molecules, 2019;24(6):. 2019 [PMID: 30884862] (Bioassay) Bioassay
NW Pensa, AS Curry, MS Reddy, SL Bellis The addition of a polyglutamate domain to the angiogenic QK peptide improves peptide coupling to bone graft materials leading to enhanced endothelial cell activation PLoS ONE, 2019;14(3):e0213592. 2019 [PMID: 30856221] (Bioassay, Human) Bioassay Human
H Xu, B Wang, M Ono, A Kagita, K Fujii, N Sasakawa, T Ueda, P Gee, M Nishikawa, M Nomura, F Kitaoka, T Takahashi, K Okita, Y Yoshida, S Kaneko, A Hotta Targeted Disruption of HLA Genes via CRISPR-Cas9 Generates iPSCs with Enhanced Immune Compatibility Cell Stem Cell, 2019;0(0):. 2019 [PMID: 30853558] (Bioassay, Human) Bioassay Human
T Colunga, M Hayworth, S Kre beta, DM Reynolds, L Chen, KL Nazor, J Baur, AM Singh, JF Loring, M Metzger, S Dalton Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 2019;26(10):2566-2579.e10. 2019 [PMID: 30840882] (Bioassay, Human) Bioassay Human
S Aghaabdoll, R Ahangari C, D Norouzian, F Davami, MR Asadi Kara, F Torkashvan, G Vaseghi, R Moazzami, S Latif Diza Enhancing bioactivity, physicochemical, and pharmacokinetic properties of a nano-sized, anti-VEGFR2 Adnectin, through PASylation technology Sci Rep, 2019;9(1):2978. 2019 [PMID: 30814652] (Bioassay, Human) Bioassay Human
N Muraoka, K Nara, F Tamura, H Kojima, H Yamakawa, T Sadahiro, K Miyamoto, M Isomi, S Haginiwa, H Tani, S Kurotsu, R Osakabe, S Torii, S Shimizu, H Okano, Y Sugimoto, K Fukuda, M Ieda Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming Nat Commun, 2019;10(1):674. 2019 [PMID: 30787297] (Bioassay, Mouse) Bioassay Mouse
S Pehrsson, M Hölttä, G Linhardt, RF Danielson, L Carlsson Rapid Production of Human VEGF-A following Intradermal Injection of Modified VEGF-A mRNA Demonstrated by Cutaneous Microdialysis in the Rabbit and Pig In Vivo Biomed Res Int, 2019;2019(0):3915851. 2019 [PMID: 30766883] (Immunoassay Standard, Transgenic Rabbit) Immunoassay Standard Transgenic Rabbit
Show All 303 Publications.

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FAQs for VEGF (293-VE). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Blogs on VEGF. Showing 1-10 of 19 blog posts - Show all blog posts.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Chemotherapy-induced metastasis: An unexpected foe?
By Yoskaly Lazo-Fernandez, PhD IntroductionEvidence has accumulated recently indicating that common cancer therapies might stimulate metastasis in a significant number of cancer patients1. In fact, neoadjuvant che...  Read full blog post.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol VEGFA
Uniprot