Recombinant Human VEGF 165 Protein, CF

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1 μg/lane of Recombinant Human VEGF165 was resolved by SDS-PAGE with silver staining, under reducing (R) and non-reducing (NR) conditions, showing major bands at 20-22 kDa and 39-42 kDa, respectively. Multiple bands in ...read more
Recombinant Human VEGF165 (Catalog # 293-VE/CF) stimulates proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human VEGF 165 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci. USA 87:1323. The ED50 for this effect is 1-6 ng/mL.
The specific activity of recombinant human VEGF165 is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF165 WHO Standard (NIBSC code: 02/286).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VEGF protein
Ala27-Arg191
Accession #
N-terminal Sequence
Ala27
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
VEGFA
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
19.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
20-22 kDa, reducing conditions
39-42 kDa, non-reducing conditions
Publications
Read Publications using
293-VE/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with trehalose.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS. Alternatively, reconstitute at 500 μg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF 165 Protein, CF

  • MVCD1
  • VAS
  • vascular endothelial growth factor A
  • Vascular permeability factor
  • Vasculotropin
  • VEGF
  • VEGFA
  • VEGF-A
  • VEGFMGC70609
  • VPF
  • VPFvascular endothelial growth factor

Background

Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult (1-3). It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure (4). Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) in length (4). VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189 (3, 4). Isoforms other than VEGF121 contain basic heparin-binding regions and are not freely diffusible (4). Human VEGF165 shares 88% aa sequence identity with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells (4). Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity (3, 4). VEGF165 binds the semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2 (5). VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells (3, 4). In adults, VEGF functions mainly in wound healing and the female reproductive cycle (3). Pathologically, it is involved in tumor angiogenesis and vascular leakage (6, 7). Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus (8). VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, oncostatin M and TNF-alpha (3, 4, 9).

  1. Leung, D.W. et al. (1989) Science 246:1306. 
  2. Keck, P.J. et al. (1989) Science 246:1309. 
  3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777. 
  4. Robinson, C.J. and S.E. Stringer (2001) J. Cell. Sci. 114:853.
  5. Pan, Q. et al. (2007) J. Biol. Chem. 282:24049.
  6. Weis, S.M. and D.A. Cheresh (2005) Nature 437:497.
  7. Thurston, G. (2002) J. Anat. 200:575.
  8. Carvalho, J.F. et al. (2007) J. Clin. Immunol. 27:246.
  9. Angelo, L.S. and R. Kurzrock (2007) Clin. Cancer Res. 13:2825.

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Publications for VEGF (293-VE/CF)(257)

We have publications tested in 15 confirmed species: Human, Mouse, Rat, Avian - Quail, Bovine, Canine, Chicken, N/A, Porcine, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Xenopus, Zebrafish.

We have publications tested in 10 applications: Binding Assay, Binding Competition, Bioassay, ELISA Standard, In Vivo, Quantum Dot, Surface Plasmon Resonance, WB Ctrl, cell culture, micro-immunoassay development.


Filter By Application
Binding Assay
(2)
Binding Competition
(1)
Bioassay
(201)
ELISA Standard
(5)
In Vivo
(30)
Quantum Dot
(1)
Surface Plasmon Resonance
(3)
WB Ctrl
(3)
cell culture
(2)
micro-immunoassay development
(1)
All Applications
Filter By Species
Human
(179)
Mouse
(26)
Rat
(9)
Avian - Quail
(1)
Bovine
(7)
Canine
(2)
Chicken
(3)
N/A
(10)
Porcine
(2)
Primate - Callitrix jacchus (Common Marmoset)
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Rabbit
(3)
Xenopus
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 257. Show All 257 Publications.
Publications using 293-VE/CF Applications Species
S Conroy, FAE Kruyt, M Wagemakers, KPL Bhat, WFA den Dunnen IL-8 associates with a pro-angiogenic and mesenchymal subtype in glioblastoma Oncotarget, 2018;9(21):15721-15731. 2018 [PMID: 29644004] (Bioassay, Human) Bioassay Human
Z Pan, J Zhuang, C Ji, Z Cai, W Liao, Z Huang Curcumin inhibits hepatocellular carcinoma growth by targeting VEGF expression Oncol Lett, 2018;15(4):4821-4826. 2018 [PMID: 29552121] (Bioassay, Mouse) Bioassay Mouse
M Chen, T Qiu, J Wu, Y Yang, GD Wright, M Wu, R Ge Extracellular anti-angiogenic proteins augment an endosomal protein trafficking pathway to reach mitochondria and execute apoptosis in HUVECs Cell Death Differ., 2018;0(0):. 2018 [PMID: 29523874] (Bioassay, Human) Bioassay Human
B Zhang, CY Wei, KK Chang, JJ Yu, WJ Zhou, HL Yang, J Shao, JJ Yu, MQ Li, F Xie TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils Oncol Lett, 2017;14(6):7483-7488. 2017 [PMID: 29344192] (Bioassay, Human) Bioassay Human
L Varinská, L Fáber, M Kello, E Petrovová, ? Balážová, P Solár, M ?oma, P Urdzík, J Mojžiš, E Švajdlenka, P Mu?aji, P Gál ?-Escin Effectively Modulates HUVECS Proliferation and Tube Formation Molecules, 2018;23(1):. 2018 [PMID: 29342121] (Bioassay, Human) Bioassay Human
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29339495] (Bioassay) Bioassay
KC Wheeler, MK Jena, BS Pradhan, N Nayak, S Das, CD Hsu, DS Wheeler, K Chen, NR Nayak VEGF may contribute to macrophage recruitment and M2 polarization in the decidua PLoS ONE, 2018;13(1):e0191040. 2018 [PMID: 29324807] (Bioassay, Human) Bioassay Human
J Cao, M Ehling, S März, J Seebach, K Tarbashevi, T Sixta, ME Pitulescu, AC Werner, B Flach, E Montanez, E Raz, RH Adams, H Schnittler Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis Nat Commun, 2017;8(1):2210. 2017 [PMID: 29263363] (Bioassay, Human) Bioassay Human
S Seo, W Suh Antiangiogenic effect of dasatinib in murine models of oxygen-induced retinopathy and laser-induced choroidal neovascularization Mol. Vis., 2017;23(0):823-831. 2017 [PMID: 29225458] (Bioassay, Human) Bioassay Human
SB Mamer, S Chen, JC Weddell, A Palasz, A Wittenkell, M Kumar, PI Imoukhuede Discovery of High-Affinity PDGF-VEGFR Interactions: Redefining RTK Dynamics Sci Rep, 2017;7(1):16439. 2017 [PMID: 29180757] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Show All 257 Publications.

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FAQs for VEGF (293-VE/CF). (Showing 1 - 1 of 1 FAQs).

  1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
    • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

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Bioinformatics Tool for VEGF (293-VE/CF)

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Blogs on VEGF. Showing 1-10 of 17 blog posts - Show all blog posts.

Getting Physical: Link between Lipid Metabolism and Hypoxia Target Genes
By Jamshed Arslan Pharm.D. von Hippel-Lindau (VHL) disease is associated with tumors arising in multiple organs. Activation of hypoxia-inducible factor (HIF)-alpha underlies the VHL disease pathogenesis. In normoxia...  Read full blog post.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

The dynamic use of a PCNA antibody in fish, porcine and primate species
Proliferating cell nuclear antigen (PCNA) plays a crucial role in nucleic acid metabolism as it pertains to DNA replication and repair.  Most noted for its activation of subunits of DNA polymerase, it has also been found to interact with cell-cycl...  Read full blog post.

The role of HIF-1 Alpha signaling in the retina under hypoxic conditions
Hypoxia inducible factor 1 (HIF-1) is a protein that plays an essential role in hypoxia, or low levels of cellular oxygen. HIF-1 is a heterodimeric protein that consists of a constitutively expressed beta subunit and oxygen related alpha subunit. ...  Read full blog post.

Using SCP3/SYCP3 Antibodies as Meiosis Markers in Gametogenesis and DNA Repair Studies
The synaptonemal complex (SC) is a protein structure that forms during the synapsis of homologous chromosomes during meiosis. This structure is involved in the processes of chromosome synapsis, genetic recombination and subsequent chromosome segregati...  Read full blog post.

Understanding the relationship between HIF-1 alpha, Hypoxia and Epithelial-Mesenchymal Transition
Epithelial-mesenchymal transition (EMT) is a natural process by which epithelial cells lose their polarity and intercellular adhesion, and gain the migratory invasive properties of mesenchymal stem cells that can differentiate into a variety of cel...  Read full blog post.

Integrin alpha v beta 3 - a target for inhibiting tumor angiogenesis
Integrins are a family of transmembrane proteins involved in diverse processes including cell adhesion, signal transduction, cell migration, and differentiation. They exist as heterodimers consisting of noncovalently linked alpha and beta subunits....  Read full blog post.

HIF-1 beta - activating gene transcription in response to hypoxia
Hypoxia-inducible factor 1 (HIF-1) is a heterodimeric transcription factor consisting of alpha and beta subunits. The levels of functional HIF-1 in the cell depends on the level of oxygen allowing cells to respond to hypoxic conditions. HIF-1a is a...  Read full blog post.

HIF-1 alpha - sensing and responding to changing oxygen levels
Hypoxia-inducible factor 1 (HIF-1) allows cells to respond to changing levels of oxygen in the environment. HIF-1 is a heterodimeric transcription factor consisting of alpha and beta subunits. Under normal conditions HIF-1 alpha is continuously sy...  Read full blog post.

Showing 1-10 of 17 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol VEGFA
Entrez
Uniprot