Recombinant Human VCAM-1/CD106 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human VCAM-1/CD106 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human sVCAM-1 coated plates (10 μg/mL with 100 μL/well), >85% will adhere after 1 hour incubation at room temperature.
Source
Chinese Hamster Ovary cell line, CHO-derived human VCAM-1/CD106 protein
Phe25-Glu698 (Arg322Gly)
Accession #
Protein/Peptide Type
Recombinant Proteins
Gene
VCAM1
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
74.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
93-95 kDa, reducing conditions
Publications
Read Publications using
ADP5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile, deionized water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VCAM-1/CD106 Protein, CF

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

Human VCAM-1 (CD106), a member of the Immunoglobulin Superfamily, is a cell surface protein expressed by activated endothelial cells and certain leukocytes (such as macrophages). VCAM-1 expression is induced by IL-1 beta, IL-4, TNF-alpha and IFN-gamma. VCAM-1 binds to leukocyte integrins VLA-4 and alpha 4 beta 7.During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1: VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

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Publications for VCAM-1/CD106 (ADP5)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using ADP5 Applications Species
Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity Sci Rep, 2016;6(0):37953. 2016 [PMID: 27892504] (Bioassay, Human) Bioassay Human
Bailon E, Ugarte-Berzal E, Amigo-Jimenez I, Van den Steen P, Opdenakker G, Garcia-Marco J, Garcia-Pardo A Overexpression of progelatinase B/proMMP-9 affects migration regulatory pathways and impairs chronic lymphocytic leukemia cell homing to bone marrow and spleen. J Leukoc Biol, 2014;96(2):185-99. 2014 [PMID: 25080557] (Bioassay, Human) Bioassay Human
Barkho BZ, Song H, Aimone JB Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation. Stem Cells Dev., 2006;15(3):407-21. 2006 [PMID: 16846377] (Bioassay, Rat) Bioassay Rat
Brooks AM, Bates ME, Vrtis RF, Jarjour NN, Bertics PJ, Sedgwick JB Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma. Am. J. Respir. Cell Mol. Biol., 2006;35(4):503-11. 2006 [PMID: 16728704] (Bioassay, Human) Bioassay Human
Yamamoto H, Nagata M, Sakamoto Y CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1. Ann. Allergy Asthma Immunol., 2005;94(2):292-300. 2005 [PMID: 15765748] (Bioassay, Human) Bioassay Human
Levesque JP, Liu F, Simmons PJ, Betsuyaku T, Senior RM, Pham C, Link DC Characterization of hematopoietic progenitor mobilization in protease-deficient mice. Blood, 2004;104(1):65-72. 2004 [PMID: 15010367] (Bioassay, Mouse) Bioassay Mouse

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Bioinformatics

Gene Symbol VCAM1
Uniprot