Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

Images

 
There are currently no images for VCAM-1/CD106 (862-VC).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to human VCAM-1 coated plates (10 µg/mL with 100 µL/well), approximately 90-100% will adhere after 1 hour incubation at RT.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human VCAM-1/CD106 protein
Met Human VCAM-1
(Phe25-Glu698)
Accession # P19320
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
VCAM1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
101.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115-130 kDa, reducing conditions
Publications
Read Publications using
862-VC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF

  • CD106 antigen
  • CD106
  • DKFZp779G2333
  • INCAM-100
  • L1CAM
  • MGC99561
  • vascular cell adhesion molecule 1
  • vascular cell adhesion protein 1
  • V-CAM 1
  • VCAM1
  • VCAM-1

Background

Human VCAM-1 (CD106, INCAM-110) is a cell adhesion molecule and a member of the immunoglobulin superfamily. Alternatively spliced forms are known to occur, but the most common form is a type I transmembrane protein with a 674 amino acid extracellular segment that includes seven C2-type immunoglobulin domains. VCAM-1 is expressed by activated endothelial cells and certain other cell types including macrophages, dendritic cells, neurons, smooth muscle cells, fibroblasts, and oocytes. VCAM-1 binds to leukocyte integrins alpha 4 beta 1 (VLA-4) and alpha 4 beta 7. During the inflammatory adhesion mechanism, activated integrins halt rolling leukocytes and attach them firmly to the vascular endothelium. They do this by binding to their ligands, for example VCAM-1, on endothelium. The VCAM-1:VLA-4/ alpha 4 beta 7 interaction is also thought to be involved in the extravasation of white blood cells through the blood vessel wall to sites of inflammation. ELISA techniques have shown that detectable levels of soluble VCAM-1 are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of VCAM-1 may be elevated or lowered in subjects with a variety of pathological conditions.

Customers Who Viewed This Item Also Viewed...

796-IC
Species: Mu
BBA16
Species: Hu
Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-45041
Species: Hu, Po
Applications: Flow, IHC, IHC-Fr, IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
AF3628
Species: Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready, ICC
BBA10
Species: Hu
Applications: WB, Simple Western, Flow, IHC, IP, CyTOF-ready
AF1730
Species: Hu
Applications: Flow, AdBlk, CyTOF-ready, ICC
NB500-328
Species: Hu
Applications: Flow, IP, CyTOF-ready
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-22122
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB110-41083
Species: Hu
Applications: WB, ChIP, ELISA, ICC/IF
862-VC
Species: Hu
Applications: Bioactivity

Publications for VCAM-1/CD106 (862-VC)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Adhesion Assay, Bioassay, Cell Culture.


Filter By Application
Adhesion Assay
(1)
Bioassay
(15)
Cell Culture
(1)
All Applications
Filter By Species
Human
(14)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using 862-VC Applications Species
A Natoni, ML Farrell, S Harris, C Falank, L Kirkham-Mc, MS Macauley, MR Reagan, M O' Dwyer Sialyltransferase inhibition leads to inhibition of tumor cell interactions with E-selectin, VCAM1, and MADCAM1, and improves survival in a human multiple myeloma mouse model Haematologica, 2019;0(0):. 2019 [PMID: 31101754] (Bioassay, Human) Bioassay Human
D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells J. Clin. Invest., 2019;129(7):2745-2759. 2019 [PMID: 31085833] (Cell Culture, Human) Cell Culture Human
JM Laufer, R Lyck, DF Legler ZAP70 expression enhances chemokine-driven chronic lymphocytic leukemia cell migration and arrest by valency regulation of integrins FASEB J., 2018;0(0):fj201701452RR. 2018 [PMID: 29589978] (Bioassay, Human) Bioassay Human
G Petkau, Y Kawano, I Wolf, M Knoll, F Melchers MiR221 promotes precursor B-cell retention in the bone marrow by amplifying the PI3K-signaling pathway in mice Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29505092] (Bioassay, Mouse) Bioassay Mouse
PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28637896] (Bioassay, Mouse) Bioassay Mouse
M Moslem, R Eggenschwi, C Wichmann, R Buhmann, T Cantz, R Henschler Kindlin-2 Modulates the Survival, Differentiation, and Migration of Induced Pluripotent Cell-Derived Mesenchymal Stromal Cells Stem Cells Int, 2017;2017(0):7316354. 2017 [PMID: 28163724] (Bioassay, Human) Bioassay Human
Sophia Koutsogian Prolonged exposure to volatile anesthetic isoflurane worsens the outcome of polymicrobial abdominal sepsis Toxicol. Sci, 2016;0(0):. 2016 [PMID: 28003439] (Bioassay, Human) Bioassay Human
Peter Adriaensen Enhanced Biosensor Platforms for Detecting the Atherosclerotic Biomarker VCAM1 Based on Bioconjugation with Uniformly Oriented VCAM1-Targeting Nanobodies Biosensors (Basel), 2016;6(3):. 2016 [PMID: 27399790] (Bioassay, Human) Bioassay Human
María Luisa Lozano Novel mutations in RASGRP2 encoding for CalDAG-GEFI abrogate Rap1 activation causing platelet dysfunction Blood, 2016;0(0):. 2016 [PMID: 27235135] (Bioassay, Human) Bioassay Human
Mukovozov I, Huang Y, Zhang Q, Liu G, Siu A, Sokolskyy Y, Patel S, Hyduk S, Kutryk M, Cybulsky M, Robinson L The Neurorepellent Slit2 Inhibits Postadhesion Stabilization of Monocytes Tethered to Vascular Endothelial Cells. J Immunol, 2015;195(7):3334-44. 2015 [PMID: 26297762] (Bioassay, Human) Bioassay Human
Show All 17 Publications.

Reviews for VCAM-1/CD106 (862-VC) (0)

There are no reviews for VCAM-1/CD106 (862-VC). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for VCAM-1/CD106 (862-VC) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human VCAM-1/CD106 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol VCAM1
Uniprot