Recombinant Human TNF-alpha Protein, CF


1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human TNF-alpha Protein, CF Summary

Additional Information
A new TNF-alpha is available! The new protein is HEK293 expressed!
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
The specific activity of Recombinant Human TNF-alpha is approximately 7.6 x 104 IU/μg, which is calibrated against human TNF-alpha WHO International Standard (NIBSC code: 88/786).   Specific activity is for reference purposes only and is not routinely tested.  
E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
17.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
17 kDa, reducing conditions
Read Publications using
210-TA/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (5 & 20 µg) is supplied as a 0.2 µm filtered solution in PBS. 
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.1-1 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein, CF

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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Publications for TNF-alpha (210-TA/CF)(673)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, Canine, Drosophila, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 16 applications: Activiation, Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Standard, Hemofiltration Procedure, IHC-P, In Vivo, Not Specified, Spheroid Culture, Standard, Stimulation, Surface Plasmon Resonance (SPR, Tissue Culture, Western Blot.

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ELISA (Capture)
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Filter By Species
Guinea Pig
Primate - Chlorocebus pygerythrus (Vervet Monkey)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 673. Show All 673 Publications.
Publications using 210-TA/CF Applications Species
KMA Abdel-Raou, R Rezgui, C Stefanini, JCM Teo, N Christofor Transdifferentiation of Human Fibroblasts into Skeletal Muscle Cells: Optimization and Assembly into Engineered Tissue Constructs through Biological Ligands Biology, 2021;10(6):. 2021 [PMID: 34208436] (Bioassay, Human) Bioassay Human
K Andelid, K Öst, A Andersson, E Mohamed, Z Jevnikar, LEGW Vanfletere, M Göransson Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis Respiratory Research, 2021;22(1):172. 2021 [PMID: 34098956] (Bioassay, Human) Bioassay Human
SR Sripathi, MW Hu, MM Liu, J Wan, J Cheng, Y Duan, JL Mertz, KJ Wahlin, J Maruotti, CA Berlinicke, J Qian, DJ Zack Transcriptome Landscape of Epithelial to Mesenchymal Transition of Human Stem Cell-Derived RPE Investigative Ophthalmology & Visual Science, 2021;62(4):1. 2021 [PMID: 33792620] (Tissue Culture, Human) Tissue Culture Human
P Rueda, J Merlin, S Chimenti, M Feletou, J Paysant, PJ White, A Christopou, PM Sexton, RJ Summers, WN Charman, LT May, CJ Langmead Pharmacological Insights Into Safety and Efficacy Determinants for the Development of Adenosine Receptor Biased Agonists in the Treatment of Heart Failure Frontiers in Pharmacology, 2021;12(0):628060. 2021 [PMID: 33776771] (Bioassay, Rat) Bioassay Rat
P Gao, P Gao, J Zhao, S Shan, W Luo, OJ Slivano, W Zhang, A Tabuchi, SA LeMaire, L Maegdefess, YH Shen, JM Miano, HA Singer, X Long MKL1 cooperates with p38MAPK to promote vascular senescence, inflammation, and abdominal aortic aneurysm Redox Biology, 2021;41(0):101903. 2021 [PMID: 33667992] (Bioassay, Human) Bioassay Human
D Biswas, P Ambalavana, M Ravins, A Anand, A Sharma, KXZ Lim, RYM Tan, HY Lim, A Sol, G Bachrach, V Angeli, E Hanski LL-37-mediated activation of host receptors is critical for defense against group A streptococcal infection Cell Reports, 2021;34(9):108766. 2021 [PMID: 33657368] (Bioassay, Human) Bioassay Human
Q Dai, Y Zhang, X Liao, Y Jiang, X Lv, X Yuan, J Meng, Y Xie, Z Peng, Q Yuan, L Tao, L Huang Fluorofenidone Alleviates Renal Fibrosis by Inhibiting Necroptosis Through RIPK3/MLKL Pathway Frontiers in Pharmacology, 2020;11(0):534775. 2020 [PMID: 33390935] (Cell Culture, Human) Cell Culture Human
NE Efstathiou, GA Moustafa, DE Maidana, EK Konstantin, S Notomi, PRT Barbisan, CD Georgakopo, JW Miller, DG Vavvas Acadesine suppresses TNF-&amp;alpha induced complement component 3 (C3), in retinal pigment epithelial (RPE) cells PLoS ONE, 2020;15(12):e0244307. 2020 [PMID: 33362238] (Bioassay, Human) Bioassay Human
PN Song, A Mansur, KJ Dugger, TR Davis, G Howard, TE Yankeelov, AG Sorace CD4 T-cell immune stimulation of HER2?+?breast cancer cells alters response to trastuzumab in vitro Cancer Cell International, 2020;20(1):544. 2020 [PMID: 33292267] (Bioassay, Human) Bioassay Human
J Stebbing, G Sánchez Ni, M Falcone, S Youhanna, P Richardson, S Ottaviani, JX Shen, C Sommerauer, G Tiseo, L Ghiadoni, A Virdis, F Monzani, LR Rizos, F Forfori, A Avendaño-C, S De Marco, L Carrozzi, F Lena, PM Sánchez-Ju, LG Lacerenza, N Cesira, D Caldevilla, A Perrella, L Niccoli, LS Méndez, D Matarrese, D Goletti, YJ Tan, V Monteil, G Dranitsari, F Cantini, A Farcomeni, S Dutta, SK Burley, H Zhang, M Pistello, W Li, M Mas Romero, F Andrés Pre, RS Simón-Tale, R García-Mol, C Kutter, JH Felce, ZF Nizami, AG Miklosi, JM Penninger, F Menichetti, A Mirazimi, P Abizanda, VM Lauschke JAK inhibition reduces SARS-CoV-2 liver infectivity and modulates inflammatory responses to reduce morbidity and mortality Sci Adv, 2020;0(0):. 2020 [PMID: 33187978] (Spheroid Culture, Human) Spheroid Culture Human
Show All 673 Publications.

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FAQs for TNF-alpha (210-TA/CF). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Gene Symbol TNF