Recombinant Human TNF-alpha Protein, CF

Images

 
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA/CF) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TNF-alpha Protein, CF Summary

Additional Information
A new economically priced TNF-alpha is available! The new protein is HEK293 expressed!
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.
Source
E. coli-derived human TNF-alpha protein
Val77-Leu233, with and without an N-terminal Met
Accession #
N-terminal Sequence
Met & Val77
Protein/Peptide Type
Recombinant Proteins
Gene
TNF
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Publications
Read Publications using
210-TA/CF in the following applications:

Packaging, Storage & Formulations

Storage
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (5 & 20 µg) is shipped with dry ice or equivalent. Upon receipt, store it immediately at -20 to -70 °C.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS. *Small pack size (5 & 20 µg) is supplied as a 0.2 µm filtered solution in PBS. 
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 0.1-1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF-alpha Protein, CF

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (1, 2). Human TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) (3). Within the ECD, human TNF-alpha shares 97% aa sequence identity with rhesus and 71%-92% with bovine, canine, cotton rat, equine, feline, mouse, porcine, and rat TNF-alpha . TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells (1, 2). TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (4). Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I (2, 5). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain (6-8). TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (9, 10) and the hematopoietic cell-restricted 80 kDa TNF RII (11, 12), both of which are also expressed as homotrimers (1, 2, 13). Both type I and type II receptors bind TNF-alpha with comparable affinity (14), although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha (15).
  1. Zelova, H. and J. Hosek (2013) Inflamm. Res. 62:641.
  2. Juhasz, K. et al. (2013) Expert Rev. Clin. Immunol. 9:335.
  3. Pennica, D. et al. (1984) Nature 312:724.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Perez, C. et al. (1990) Cell 63:251.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Gearing, A.J.H. et al. (1994) Nature 370:555.
  9. Schall, T.J. et al. (1990) Cell 61:361.
  10. Loetscher, H. et al. (1990) Cell 61:351.
  11. Dembic, Z. et al. (1990) Cytokine 2:231.
  12. Smith, C.A. et al. (1990) Science 248:1019.
  13. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  14. Pinckard, J.K. et al. (1997) J. Biol. Chem. 272:10784.
  15. Engelmann, H. et al. (1990) J. Biol. Chem. 265:1531.

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210-TA/CF
Species: Hu
Applications: Bioactivity

Publications for TNF-alpha (210-TA/CF)(739)

We have publications tested in 16 confirmed species: Human, Mouse, Rat, Bovine, Canine, Drosophila, Feline, Guinea Pig, Hamster, N/A, Ovine, Porcine, Primate - Chlorocebus pygerythrus (Vervet Monkey), Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Rabbit.

We have publications tested in 16 applications: Activiation, Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Standard, Hemofiltration Procedure, IHC-P, In Vivo, Not Specified, Spheroid Culture, Standard, Stimulation, Surface Plasmon Resonance (SPR, Tissue Culture, Western Blot.


Filter By Application
Activiation
(1)
Bioassay
(655)
Cell Culture
(37)
ELISA (Capture)
(1)
ELISA (Standard)
(13)
ELISA Standard
(2)
Hemofiltration Procedure
(1)
IHC-P
(1)
In Vivo
(4)
Not Specified
(2)
Spheroid Culture
(1)
Standard
(1)
Stimulation
(3)
Surface Plasmon Resonance (SPR
(1)
Tissue Culture
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(658)
Mouse
(25)
Rat
(8)
Bovine
(6)
Canine
(2)
Drosophila
(1)
Feline
(1)
Guinea Pig
(1)
Hamster
(1)
N/A
(13)
Ovine
(1)
Porcine
(1)
Primate - Chlorocebus pygerythrus (Vervet Monkey)
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(4)
Rabbit
(1)
All Species
Showing Publications 1 - 10 of 739. Show All 739 Publications.
Publications using 210-TA/CF Applications Species
HJ Kim, SH Kim, YS Oh, SR Lee, HD Chae Dienogest May Reduce Estradiol- and Inflammatory Cytokine-Induced Cell Viability and Proliferation and Inhibit the Pathogenesis of Endometriosis: A Cell Culture- and Mouse Model-Based Study Biomedicines, 2022;10(11):. 2022 [PMID: 36428561] (Cell Culture, Human) Cell Culture Human
K Li, Y Zhu, P Zhang, M Alini, S Grad, Z Li Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models Journal of Orthopaedic Translation, 2023;38(0):106-116. 2023 [PMID: 36381242] (Cell Culture, Human) Cell Culture Human
P Pavlidis, A Tsakmaki, A Treveil, K Li, D Cozzetto, F Yang, U Niazi, BH Hayee, M Saqi, J Friedman, T Korcsmaros, G Bewick, N Powell Cytokine responsive networks in human colonic epithelial organoids unveil a molecular classification of inflammatory bowel disease Cell Reports, 2022;40(13):111439. 2022 [PMID: 36170836] (Bioassay, Human) Bioassay Human
H Iwanishi, O Yamanaka, T Sumioka, S Yasuda, M Miyajima, S Saika Delayed regression of laser-induced choroidal neovascularization in TNFalpha-null mice Journal of Cellular and Molecular Medicine, 2022;0(0):. 2022 [PMID: 36127870] (Bioassay, Human) Bioassay Human
V Catalán, P Domench, J Gómez-Ambr, B Ramírez, S Becerril, A Mentxaka, A Rodríguez, V Valentí, R Moncada, J Baixauli, C Silva, J Escalada, G Frühbeck Dermatopontin Influences the Development of Obesity-Associated Colon Cancer by Changes in the Expression of Extracellular Matrix Proteins International Journal of Molecular Sciences, 2022;23(16):. 2022 [PMID: 36012487] (Stimulation, Human) Stimulation Human
DC Humphries, R Mills, C Boz, BJ McHugh, N Hirani, AG Rossi, A Pedersen, HT Schambye, RJ Slack, H Leffler, UJ Nilsson, W Wang, T Sethi, AC Mackinnon Galectin-3 inhibitor GB0139 protects against acute lung injury by inhibiting neutrophil recruitment and activation Frontiers in Pharmacology, 2022;13(0):949264. 2022 [PMID: 36003515] (Bioassay, Human) Bioassay Human
YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022;8(34):eabn5522. 2022 [PMID: 36001668] (Bioassay, Human) Bioassay Human
Z Cai, S Tian, T Klein, L Tu, LW Geenen, T Koudstaal, AE van den Bo, YB de Rijke, IKM Reiss, E Boersma, C van der Le, M Van Faasse, I Kema, DJ Duncker, KA Boomars, K Tran-Lundm, C Guignabert, D Merkus Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha Scientific Reports, 2022;12(1):12326. 2022 [PMID: 35853948] (Bioassay, Human) Bioassay Human
XJ Mi, JK Kim, S Lee, SK Moon, YJ Kim, H Kim In vitro assessment of the anti-inflammatory and skin-moisturizing effects of Filipendula palmata (Pall.) Maxim. On human keratinocytes and identification of its bioactive phytochemicals Journal of ethnopharmacology, 2022;296(0):115523. 2022 [PMID: 35809756] (Bioassay, Human) Bioassay Human
MD Gundersen, KB Larsen, KM Johnsen, R Goll, J Florholmen, G Haraldsen Hypo-osmotic stress induces the epithelial alarmin IL-33 in the colonic barrier of ulcerative colitis Scientific Reports, 2022;12(1):11550. 2022 [PMID: 35798804] (Bioassay, Human) Bioassay Human
Show All 739 Publications.

Reviews for TNF-alpha (210-TA/CF) (0)

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FAQs for TNF-alpha (210-TA/CF). (Showing 1 - 4 of 4 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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Blogs on TNF-alpha. Showing 1-10 of 12 blog posts - Show all blog posts.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.


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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol TNF
Uniprot