Recombinant Human Thrombospondin-2 Protein, CF


Recombinant Human Thrombospondin-2 His-tag (Catalog # 1635-T2) supports the adhesion of SVEC4-10 vascular endothelial cells. The ED50 for this effect is 0.07-0.7 μg/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human Thrombospondin-2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.

The ED50 for this effect is 0.07-0.7 μg/mL.

Mouse myeloma cell line, NS0-derived human Thrombospondin-2 protein
Gly19-Ile1172, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
170-176 kDa, reducing conditions
Read Publications using
1635-T2 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombospondin-2 Protein, CF

  • THBS2
  • thrombospondin 2
  • Thrombospondin2
  • Thrombospondin-2
  • TSP-2
  • TSP2thrombospondin-2


Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1‑4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal heparin‑binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88‑90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C-terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin‑binding domain is responsible for the destabilization of focal adhesion sites (8‑10). The heparin‑binding domain also mediates binding to Integrins alpha 3 beta 1 and alpha 6 beta 1 on microvascular endothelial cells (EC) and Integrin alpha 4 beta 1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin‑binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).

  1. Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
  2. Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
  3. Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
  4. Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
  5. LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
  6. Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
  7. Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
  8. Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
  9. Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
  10. Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
  11. Calzada, M.J. et al. (2004) Circ. Res. 94:462.
  12. Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
  13. Noh, Y.-H. et al. (2003) J. Invest. Dermatol. 121:1536.
  14. Simantov, R. et al. (2005) Matrix Biol. 24:27.
  15. Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
  16. Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.

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Publications for Thrombospondin-2 (1635-T2)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Western Blot Control.

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In Vivo
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Showing Publications 1 - 9 of 9.
Publications using 1635-T2 Applications Species
H Yang, B Sun, L Fan, W Ma, K Xu, SRR Hall, Z Wang, RA Schmid, RW Peng, TM Marti, W Gao, J Xu, W Yang, F Yao Multi-scale integrative analyses identify THBS2+ cancer-associated fibroblasts as a key orchestrator promoting aggressiveness in early-stage lung adenocarcinoma Theranostics, 2022;12(7):3104-3130. 2022 [PMID: 35547750] (In Vivo, Mouse) In Vivo Mouse
NMP Cartee, SJ Lee, SG Keep, MM Wang Context-dependent monoclonal antibodies against protein carbamidomethyl-cysteine PLoS ONE, 2020;15(11):e0242376. 2020 [PMID: 33232360] (Western Blot Control, Human) Western Blot Control Human
JF Liu, PC Chen, TM Chang, CH Hou Thrombospondin-2 stimulates MMP-9 production and promotes osteosarcoma metastasis via the PLC, PKC, c-Src and NF-&amp;kappaB activation J Cell Mol Med, 2020;0(0):. 2020 [PMID: 33021341] (Cell Culture, Human) Cell Culture Human
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018;16(1):63. 2018 [PMID: 29534722] (Bioassay, Human) Bioassay Human
F Roudnicky, SY Yoon, S Poghosyan, S Schwager, C Poyet, G Vella, SB Bachmann, S Karaman, JW Shin, VI Otto, M Detmar Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels Oncogene, 2018;0(0):. 2018 [PMID: 29467494] (Bioassay, Human) Bioassay Human
S Wang, T Hu, Z Wang, N Li, L Zhou, L Liao, M Wang, L Liao, H Wang, L Zeng, C Fan, H Zhou, K Xiong, J Huang, D Chen Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure PLoS ONE, 2017;12(9):e0185388. 2017 [PMID: 28953973] (Bioassay, Rat) Bioassay Rat
PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression J Hematol Oncol, 2017;10(1):33. 2017 [PMID: 28122633] (Bioassay, Human) Bioassay Human
Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome. Hum Mol Genet, 2014;23(23):6177-90. 2014 [PMID: 24990151] (Bioassay, Human) Bioassay Human
Benner , Eric J, Luciano , Dominic, Jo , Rebecca, Abdi , Khadar, Paez-Gonzalez , Patricia, Sheng , Huaxin, Warner , David S, Liu , Chunlei, Eroglu , Cagla, Kuo , Chay T Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013;497(7449):369-73. 2013 [PMID: 23615612] (Bioassay, N/A) Bioassay N/A

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Gene Symbol THBS2