Recombinant Human Thrombospondin-2 Protein, CF

Images

 
There are currently no images for Thrombospondin-2 (1635-T2).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Thrombospondin-2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. Recombinant Human Thrombospondin‑2 (rhThrombospondin-2) is coated to 96 well plates at 10 µg/mL (100 µL/well) overnight then reduced with 20 mM DTT for 30 minutes. When 2 x 104 cells/well are added to rhThrombospondin-2 coated plates, >55% will adhere after one hour at 37 °C.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Thrombospondin-2 protein
Gly19-Ile1172, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Gly19
Protein/Peptide Type
Recombinant Proteins
Gene
THBS2
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
170-176 kDa, reducing conditions
Publications
Read Publications using
1635-T2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombospondin-2 Protein, CF

  • THBS2
  • thrombospondin 2
  • Thrombospondin2
  • Thrombospondin-2
  • TSP-2
  • TSP2thrombospondin-2

Background

Thrombospondin-2 (TSP-2) is a 150 kDa calcium-binding protein that modulates cellular interactions with extracellular matrix. Thrombospondin-1 and -2 constitute subgroup A thrombospondin family members and form disulfide-linked homotrimers, whereas Thrombospondin-3, -4, and -5/COMP constitute subgroup B and form homopentamers (1‑4). The human TSP-2 cDNA encodes a 1172 amino acid (aa) precursor that includes an 18 aa signal sequence followed by an N-terminal heparin‑binding domain, an oligomerization motif, one vWF-C domain, three TSP type-1 repeats, three EGF-like repeats, seven TSP type-3 repeats, and a lectin-like TSP C‑terminal domain (5). Human TSP-2 shares 88‑90% aa sequence identity with bovine, mouse, and rat TSP-2. Within the TSP type-3 repeats and TSP C-terminal domain, human TSP-2 shares 80% aa sequence identity with human TSP-1 and approximately 60% aa sequence identity with human TSP-3, -4, and -5/COMP. TSP-2 regulates collagen matrix formation by altering fibroblast behavior during development and in areas of tissue remodeling in the adult (6, 7). Trimerization of TSP-2 is required for the calcium-dependent cell attachment and spreading functions, while the heparin‑binding domain is responsible for the destabilization of focal adhesion sites (8‑10). The heparin‑binding domain also mediates binding to Integrins alpha 3 beta 1 and alpha 6 beta 1 on microvascular endothelial cells (EC) and Integrin alpha 4 beta 1 on large blood vessel EC (11, 12). A fragment of TSP-2 (heparin‑binding domain, oligomerization motif, and vWF-C domain) promotes EC survival, proliferation, and chemotaxis (11). Inclusion of the three TSP type-1 domains results in a molecule that inhibits VEGF-induced EC migration and vascular tube formation (13, 14). In vivo, full length TSP-2 blocks tumor angiogenesis and induces vascular EC apoptosis (13, 15). HPRG functions as an apparent decoy receptor by preventing interaction of TSP-2 with CD36 on macrophages and microvasculature EC (14). TSP-2 also binds MMP-2 and facilitates MMP-2 clearance by the scavenger receptor LRP (16).

  1. Elzie, C.A. and J.E. Murphy-Ullrich (2004) Int. J. Biochem. Cell Biol. 36:1090.
  2. Armstrong, L.C. and P. Bornstein (2003) Matrix Biol. 22:63.
  3. Murphy-Ullrich, J.E. (2001) J. Clin. Invest. 107:785.
  4. Bornstein, P. and E.H. Sage (2002) Curr. Opin. Cell Biol. 14:608.
  5. LaBell, T.L. and P.H. Byers (1993) Genomics 17:225.
  6. Kyriakides, T.R. et al. (1998) J. Histochem. Cytochem. 46:1007.
  7. Kyriakides, T.R. et al. (1998) J. Cell Biol. 140:419.
  8. Anilkumar, N. et al. (2002) J. Cell Sci. 115:2357.
  9. Misenheimer, T.M. et al. (2003) Biochemistry 42:5125.
  10. Murphy-Ullrich, J.E. et al. (1993) J. Biol. Chem. 268:26784.
  11. Calzada, M.J. et al. (2004) Circ. Res. 94:462.
  12. Calzada, M.J. et al. (2003) J. Biol. Chem. 278:40679.
  13. Noh, Y.-H. et al. (2003) J. Invest. Dermatol. 121:1536.
  14. Simantov, R. et al. (2005) Matrix Biol. 24:27.
  15. Streit, M. et al. (1999) Proc. Natl. Acad. Sci. 96:14888.
  16. Yang, Z. et al. (2001) J. Biol. Chem. 276:8403.

Customers Who Viewed This Item Also Viewed...

NBP2-24582
Species: Hu, Mu, Rt, Pm
Applications: WB, IHC, IHC-P
NBP1-52012
Species: Hu, Po, Ca
Applications: WB, IHC, IHC-P, PEP-ELISA
AF942
Species: Mu
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
AF2390
Species: Hu
Applications: WB, Simple Western
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-30027
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
1635-T2
Species: Hu
Applications: Bioactivity

Publications for Thrombospondin-2 (1635-T2)(6)

We have publications tested in 3 confirmed species: Human, Rat, N/A.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(6)
All Applications
Filter By Species
Human
(4)
Rat
(1)
N/A
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 1635-T2 Applications Species
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018;16(1):63. 2018 [PMID: 29534722] (Bioassay, Human) Bioassay Human
F Roudnicky, SY Yoon, S Poghosyan, S Schwager, C Poyet, G Vella, SB Bachmann, S Karaman, JW Shin, VI Otto, M Detmar Alternative transcription of a shorter, non-anti-angiogenic thrombospondin-2 variant in cancer-associated blood vessels Oncogene, 2018;0(0):. 2018 [PMID: 29467494] (Bioassay, Human) Bioassay Human
S Wang, T Hu, Z Wang, N Li, L Zhou, L Liao, M Wang, L Liao, H Wang, L Zeng, C Fan, H Zhou, K Xiong, J Huang, D Chen Macroglia-derived thrombospondin 2 regulates alterations of presynaptic proteins of retinal neurons following elevated hydrostatic pressure PLoS ONE, 2017;12(9):e0185388. 2017 [PMID: 28953973] (Bioassay, Rat) Bioassay Rat
PC Chen, CH Tang, LW Lin, CH Tsai, CY Chu, TH Lin, YL Huang Thrombospondin-2 promotes prostate cancer bone metastasis by the up-regulation of matrix metalloproteinase-2 through down-regulating miR-376c expression J Hematol Oncol, 2017;10(1):33. 2017 [PMID: 28122633] (Bioassay, Human) Bioassay Human
Hellbach N, Weise S, Vezzali R, Wahane S, Heidrich S, Roidl D, Pruszak J, Esser J, Vogel T Neural deletion of Tgfbr2 impairs angiogenesis through an altered secretome. Hum Mol Genet, 2014;23(23):6177-90. 2014 [PMID: 24990151] (Bioassay, Human) Bioassay Human
Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013;497(7449):369-73. 2013 [PMID: 23615612] (Bioassay, N/A) Bioassay N/A

Reviews for Thrombospondin-2 (1635-T2) (0)

There are no reviews for Thrombospondin-2 (1635-T2). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Thrombospondin-2 (1635-T2) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Thrombospondin-2 Products

Bioinformatics Tool for Thrombospondin-2 (1635-T2)

Discover related pathways, diseases and genes to Thrombospondin-2 (1635-T2). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Thrombospondin-2 (1635-T2)

Discover more about diseases related to Thrombospondin-2 (1635-T2).
 

Pathways for Thrombospondin-2 (1635-T2)

View related products by pathway.

PTMs for Thrombospondin-2 (1635-T2)

Learn more about PTMs related to Thrombospondin-2 (1635-T2).

Blogs on Thrombospondin-2

There are no specific blogs for Thrombospondin-2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Thrombospondin-2 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol THBS2
Uniprot