<0.10 EU per 1 μg of the protein by the LAL method.
103 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
100-145 kDa, reducing conditions
Read Publication using 8390-TH in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and CHAPS.
>90%, by SDS-PAGE with silver staining.
Reconstitute at 200 μg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human Thrombospondin-3 Protein, CF
Thrombospondin-3 (TSP-3) is a 140-150 kDa heparin-binding protein in the thrombospondin family of matricellular adhesion proteins. Within this family, TSP-3, TSP-4, and TSP-5/COMP are secreted as pentamers, while TSP-1 and TSP-2 are homotrimeric (1). TSP-3 contains an N-terminal Laminin G-like globular domain, a flexible linker region, multiple tandem EGF-like repeats, multiple tandem TSP type-3 repeats, and a globular TSP C-terminal domain (2). Alternative splicing of human TSP-3 generates a short isoform with a deletion of approximately half of the Laminin-like domain and a portion of the linker. Mature human TSP-3 shares 98% and 97% amino acid sequence identity with mouse and rat TSP-3, respectively. In the disulfide-linked TSP-3 pentamer, the Laminin-like domains are clustered while the C-terminal globular domains are extended (3). TSP-3 is expressed during mouse embryogenesis in the brain, dorsal root ganglia, cartilage, lung, and gut (4, 5). In postnatal mice it is most highly expressed in the lung (6). TSP-3 is involved in the columnar organization of chondrocytes in developing growth plates of long bones (7). It is up-regulated in metastatic osteosarcoma and in the corneal stroma during wound healing (8, 9).
Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
Adolph, K.W. et al. (1995) Genomics 27:329.
Qabar, A. et al. (1995) J. Biol. Chem. 270:12725.
Iruela-Arispe, M.L. et al. (1993) Dev. Dyn. 197:40.
Qabar, A. et al. (1994) J. Biol. Chem. 269:1262.
Vos, H.L. et al. (1992) J. Biol. Chem. 267:12192.
Posey, K.L. et al. (2008) Am. J. Pathol. 172:1664.
Dalla-Torre, C.A. et al. (2006) BMC Cancer 6:237.
Armstrong, D.J. et al. (2002) Int. J. Biochem. Cell Biol. 34:588.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human Thrombospondin-3 Protein, CF and receive a gift card or discount.