Recombinant Human Thrombospondin-3 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Thrombospondin-3 Protein, CF Summary

Details of Functionality
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.3-1.5 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Thrombospondin-3 protein
Ser22-Val956 with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Ser22
Structure / Form
Disulfide-linked oligomer
Protein/Peptide Type
Recombinant Proteins
Gene
THBS3
Purity
>90%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
100-145 kDa, reducing conditions
Publications
Read Publication using
8390-TH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, NaCl and CHAPS.
Purity
>90%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Thrombospondin-3 Protein, CF

  • MGC119565
  • THBS3
  • thrombospondin 3
  • Thrombospondin3
  • Thrombospondin-3
  • TSP3
  • TSP3MGC119564

Background

Thrombospondin-3 (TSP-3) is a 140-150 kDa heparin-binding protein in the thrombospondin family of matricellular adhesion proteins. Within this family, TSP-3, TSP-4, and TSP-5/COMP are secreted as pentamers, while TSP-1 and TSP-2 are homotrimeric (1). TSP-3 contains an N-terminal Laminin G-like globular domain, a flexible linker region, multiple tandem EGF-like repeats, multiple tandem TSP type-3 repeats, and a globular TSP C-terminal domain (2). Alternative splicing of human TSP-3 generates a short isoform with a deletion of approximately half of the Laminin-like domain and a portion of the linker. Mature human TSP-3 shares 98% and 97% amino acid sequence identity with mouse and rat TSP-3, respectively. In the disulfide-linked TSP-3 pentamer, the Laminin-like domains are clustered while the C-terminal globular domains are extended (3). TSP-3 is expressed during mouse embryogenesis in the brain, dorsal root ganglia, cartilage, lung, and gut (4, 5). In postnatal mice it is most highly expressed in the lung (6). TSP-3 is involved in the columnar organization of chondrocytes in developing growth plates of long bones (7). It is up-regulated in metastatic osteosarcoma and in the corneal stroma during wound healing (8, 9).
  1. Murphy-Ullrich, J.E. and R.V. Iozzo (2012) Matrix Biol. 31:152.
  2. Adolph, K.W. et al. (1995) Genomics 27:329.
  3. Qabar, A. et al. (1995) J. Biol. Chem. 270:12725.
  4. Iruela-Arispe, M.L. et al. (1993) Dev. Dyn. 197:40.
  5. Qabar, A. et al. (1994) J. Biol. Chem. 269:1262.
  6. Vos, H.L. et al. (1992) J. Biol. Chem. 267:12192.
  7. Posey, K.L. et al. (2008) Am. J. Pathol. 172:1664.
  8. Dalla-Torre, C.A. et al. (2006) BMC Cancer 6:237.
  9. Armstrong, D.J. et al. (2002) Int. J. Biochem. Cell Biol. 34:588.

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Publications for Thrombospondin-3 (8390-TH)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol THBS3
Uniprot