CRISP-2 Antibody Summary
Peptide with sequence C-QHSDPEDRKTSTR corresponding to internal region according to NP_003287.1.
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Immunogen affinity purified
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- Western Blot 1:1000
- Peptide ELISA Detection limit 1:8000
Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Primary incubations were for 1 hour.
Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide
IHC: Paraffin embedded Human Testis.
Expected from sequence similarity: Porcine, Canine
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for CRISP-2 Antibody
- Cancer/testis antigen 36
- cysteine-rich secretory protein 2
- glyceraldehyde-3-phosphate dehydrogenase-like 5
- testis specific protein 1 (probe H4-1 p3-1)
- Testis-specific protein TPX-1
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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