CRISP-2 Antibody

Images

 
Western Blot: CRISP-2 Antibody [NBP1-52012] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane ...read more

Product Details

Summary
Reactivity Hu, Po, CaSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

CRISP-2 Antibody Summary

Immunogen
Peptide with sequence C-QHSDPEDRKTSTR corresponding to internal region according to NP_003287.1.
Epitope
C-QHSDPEDRKTSTR
Specificity
Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CRISP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: In transfected HEK293 transiently expressing human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Preliminary experiments gave an approx. 55 kDa band in human testis lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3 kDa band according to NP_003287.1. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.
Publications
Read Publication using NBP1-52012.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for CRISP-2 Antibody

  • Cancer/testis antigen 36
  • CRISP2
  • CRISP-2
  • CRISP-2GAPDL5
  • CT36TSP1
  • cysteine-rich secretory protein 2
  • GAPDL5
  • glyceraldehyde-3-phosphate dehydrogenase-like 5
  • testis specific protein 1 (probe H4-1 p3-1)
  • Testis-specific protein TPX-1
  • TPX1
  • TPX1MGC111136
  • TSP1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CRISP-2 Antibody (NBP1-52012)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CRISP2
Entrez
Uniprot