Human COMP Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human COMP Quantikine ELISA Kit Summary

• Background: The Quantikine Human COMP immunoassay is a 4.5 hour solid phase ELISA designed to measure human COMP in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human COMP and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human COMP showed linear curves that were parallel to the standard curves obtained u...sing the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring COMP.
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• Specificity: Natural and recombinant human COMP
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: COMP

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human COMP Quantikine ELISA Kit

• cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• cartilage oligomeric matrix protein
• cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple)
• COMP
• EDM1
• EPD1
• MED
• MEDMGC131819
• MGC149768
• PSACH
• pseudoachondroplasia (epiphyseal dysplasia 1, multiple)
• THBS5
• Thrombospondin5
• Thrombospondin-5
• TSP5

Background

COMP (Cartilage Oligomeric Matrix Protein), also known as Thrombospondin-5, is a multidomain calcium binding protein that associates with other extracellular matrix molecules. COMP associates into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands. An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism. An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrins a5b1 or aVb3. COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas. Several mutations in COMP are associated with skeletal dysplasias.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for COMP/Thrombospondin-5 (DCMP0)(12)

Publications using DCMP0

• Nishida, Y;Hashimoto, Y;Orita, K;Nishino, K;Kinoshita, T;Iida, K;Nakamura, H; Longitudinal measurement of serum cartilage oligomeric matrix protein can detect the progression of cartilage degeneration in anterior cruciate ligament reconstruction patients Asia-Pacific journal of sports medicine, arthroscopy, rehabilitation and technology 2024-07-01 [PMID: 39091893] (Human)
• HJ Kim, HC Lee The Effect of 13 Weeks Long-Distance Bicycle Riding on Inflammatory Response Indicators Related to Joint Cartilage and Muscle Damage International Journal of Environmental Research and Public Health, 2022-12-06;19(23):. 2022-12-06 [PMID: 36498391] (Human)
• C Papaneophy, A Alabajos-C, E Viosca-Her, C Calvis, M Costa, AE Christodou, A Kroushovsk, A Lapithis, VM Lapithi, I Papayianni, A Christou, R Messeguer, C Giannaki, K Felekkis Associations between serum biomarkers of cartilage metabolism and serum hyaluronic acid, with risk factors, pain categories, and disease severity in knee osteoarthritis: a pilot study Bmc Musculoskeletal Disorders, 2022-03-02;23(1):195. 2022-03-02 [PMID: 35236298] (Human)
• SW Jhan, CJ Wang, KT Wu, KK Siu, JY Ko, WC Huang, WY Chou, JH Cheng Comparison of Extracorporeal Shockwave Therapy with Non-Steroid Anti-Inflammatory Drugs and Intra-Articular Hyaluronic Acid Injection for Early Osteoarthritis of the Knees Biomedicines, 2022-01-18;10(2):. 2022-01-18 [PMID: 35203417] (Human)
• Y Fu, Y Huang, Z Yang, Y Chen, J Zheng, C Mao, Z Li, Z Liu, B Yu, T Li, M Wang, C Xu, Y Zhou, G Zhao, Y Jia, W Guo, X Jia, T Zhang, L Li, Z Liu, S Guo, M Ma, H Zhang, B Liu, J Du, W Wang, C Tang, P Gao, Q Xu, X Wang, J Liu, J Sun, W Kong Cartilage oligomeric matrix protein is an endogenous &beta-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury Cell Research, 2021-01-28;0(0):. 2021-01-28 [PMID: 33510386] (Human)
• K Winsz-Szcz, K Ku?nik-Tro, A Gruenpeter, M Wojdas, K D?bkowska, K Olczyk Association of Circulating COMP and YKL-40 as Markers of Metabolic Changes of Cartilage with Adipocytokines in Juvenile Idiopathic Arthritis Metabolites, 2020-02-10;10(2):. 2020-02-10 [PMID: 32050571] (Human)
• L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP J. Exp. Clin. Cancer Res., 2019-04-18;38(1):170. 2019-04-18 [PMID: 30999932] (Human)
• VN Nfonsam, H Jecius, D Chen, PN Omesiete, AN Ewongwo, E Elquza, AJ Scott, J Jandova Increasing Incidence of Colon Cancer in the Young: Assessing the Tumor Biology J. Am. Coll. Surg., 2019-04-14;0(0):. 2019-04-14 [PMID: 30995524] (Human)
• S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12 J. Cell. Mol. Med., 2019-03-22;0(0):. 2019-03-22 [PMID: 30903650] (Human)
• M Azukizawa, H Ito, Y Hamamoto, T Fujii, Y Morita, A Okahata, T Tomizawa, M Furu, K Nishitani, S Kuriyama, S Nakamura, H Yoshitomi, T Nakatani, T Tsuboyama, M Hamaguchi, S Matsuda, T Yasuda The Effects of Well-Rounded Exercise Program on Systemic Biomarkers Related to Cartilage Metabolism Cartilage, 2018-04-12;0(0):1947603518767. 2018-04-12 [PMID: 29644876] (Human)
• HL Lopez, SM Habowski, JE Sandrock, B Raub, A Kedia, EJ Bruno, TN Ziegenfuss Effects of dietary supplementation with a standardized aqueous extract of Terminalia chebula fruit (AyuFlex(�)) on joint mobility, comfort, and functional capacity in healthy overweight subjects: a randomized placebo-controlled clinical trial BMC Complement Altern Med, 2017-10-02;17(1):475. 2017-10-02 [PMID: 28969626] (Human)
• E Das Gupta, WR Ng, SF Wong, AK Bhurhanude, SS Yeap Correlation of serum cartilage oligometric matrix protein (COMP) and interleukin-16 (IL-16) levels with disease severity in primary knee osteoarthritis: A pilot study in a Malaysian population PLoS ONE, 2017-09-14;12(9):e0184802. 2017-09-14 [PMID: 28910372] (Human)

FAQs for COMP/Thrombospondin-5 (DCMP0). (Showing 1 - 2 of 2 FAQs).

1. I have synovial fluid sample and I want to work on Western bloting can you support me ,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
   • We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
2. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COMP