Thrombospondin-4 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human heart tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Thrombospondin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2390) followed by ...read more
Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Thrombospondin‑4 at approximately 158 kDa (as indicated) using 2 µg/mL of Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Thrombospondin-4 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombospondin-4
Ala22-Asn961 (Pro276Ala, Ala420Val)
Accession # P35443
Specificity
Detects human Thrombospondin-4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Thrombospondin-4 is observed and less than 5% cross-reactivity with recombinant human (rh) Thrombospondin-1, rhThrombospondin-2, and rhThrombospondin-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
THBS4
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Publications
Read Publications using
AF2390 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombospondin-4 Antibody [Unconjugated]

  • THBS4
  • thrombospondin 4
  • Thrombospondin4
  • Thrombospondin-4
  • TSP4
  • TSP-4

Background

Thrombospondin-4 (THSP4) is a 140 kDa calcium-binding protein that interacts with other extracellular matrix molecules and modulates the activity of various cell types. THSP1 and THSP2 constitute subgroup A and form disulfide-linked homotrimers, whereas THSP3, THSP4, and THSP5/COMP constitute subgroup B and form pentamers (1, 2). The human THSP4 cDNA encodes a 961 amino acid (aa) precursor that includes a 26 aa signal sequence followed by an N-terminal heparin-binding domain, a coiled-coil motif, four EGF-like repeats, seven THSP type-3 repeats (one with an RGD motif), and a THSP C‑terminal domain (3). Human THSP4 shares 93% aa sequence identity with mouse and rat THSP4. Within the THSP type-3 repeats and the THSP C‑terminal domain, human THSP4 shares 79% aa sequence identity with THSP3 and COMP, and 58% aa sequence identity with THSP1 and THSP2. The coiled-coil motif mediates pentamer formation with COMP, either homotypically or heterotypically (3-6). THSP4 binds a variety of matrix proteins including collagens I, II, III, V, laminin-1, fibronectin, and matrilin-2 (4). Interactions of THSP4 with non-collagenous proteins are independent of divalent cations, while interactions with collagenous proteins are enhanced in the presence of zinc (4). THSP4 is expressed in heart, skeletal muscle, vascular smooth muscle, and vascular endothelial cells (7-9). It accumulates at neuromuscular junctions and synapse-rich regions and is upregulated in muscle by experimental denervation (8). THSP4 mediates the adhesion of motor and sensory neurons and promotes neurite outgrowth (8). A polymorphism of THSP4 (A387P) is associated with early coronary artery disease (10-12). Unlike wild type THSP4, the A387P variant does not support HUVEC attachment and spreading (9). Integrin alpha M/ beta 2 enables activated neutrophil adhesion to both the variant A387P and wild type THSP4, although the A387P variant induces a greater release of pro-inflammatory molecules (13).

  1. Adams, J.C. and J. Lawler (2004) Int. J. Biochem. Cell Biol. 36:961.
  2. Stenina, O.I. et al. (2004) Int. J. Biochem. Cell Biol. 36:1013.
  3. Lawler, J. et al. (1995) J. Biol. Chem. 270:2809.
  4. Narouz-Ott, L. et al. (2000) J. Biol. Chem. 275:37110.
  5. Hauser, N. et al. (1995) FEBS Lett. 368:307.
  6. Sodersten, F. et al. (2006) Connect. Tissue Res. 47:85.
  7. Lawler, J. et al. (1993) J. Cell Biochem. 120:1059.
  8. Arber, S. and P. Caroni (1995) J. Cell Biol. 131:1083.
  9. Stenina, O.I. et al. (2003) Circulation 108:1514.
  10. Topol, E.J. et al. (2001) Circulation 104:2641.
  11. Wessel, J. et al. (2004) Am. Heart J. 147:905.
  12. Stenina, O.I. et al. (2005) FASEB J. 19:1893.
  13. Pluskota, E. et al. (2005) Blood 106:3970.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thrombospondin-4 Antibody (AF2390)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, IHC-P, Western Blot.


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IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(4)
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Human
(2)
Mouse
(5)
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Showing Publications 1 - 7 of 7.
Publications using AF2390 Applications Species
S Muppala, R Xiao, I Krukovets, D Verbovetsk, R Yendamuri, N Habib, P Raman, E Plow, O Stenina-Ad Thrombospondin-4 mediates TGF-?-induced angiogenesis Oncogene, 2017;0(0):. 2017 [PMID: 28481870] (IHC, Mouse) IHC Mouse
Thrombospondin expression in myofibers stabilizes muscle membranes Elife, 2016;5(0):. 2016 [PMID: 27669143] (Western Blot, Mouse) Western Blot Mouse
Assessing the contribution of thrombospondin-4 induction and ATF6? activation to endoplasmic reticulum expansion and phenotypic modulation in bladder outlet obstruction Sci Rep, 2016;6(0):32449. 2016 [PMID: 27581066] (Western Blot, Mouse) Western Blot Mouse
Girard F, Eichenberger S, Celio M Thrombospondin 4 deficiency in mouse impairs neuronal migration in the early postnatal and adult brain. Mol Cell Neurosci, 2014;61(0):176-86. 2014 [PMID: 24983516] (IHC-P, Mouse) IHC-P Mouse
Benner , Eric J, Luciano , Dominic, Jo , Rebecca, Abdi , Khadar, Paez-Gonzalez , Patricia, Sheng , Huaxin, Warner , David S, Liu , Chunlei, Eroglu , Cagla, Kuo , Chay T Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4. Nature, 2013;497(7449):369-73. 2013 [PMID: 23615612] (Western Blot, Mouse) Western Blot Mouse
Forster S, Gretschel S, Jons T, Yashiro M, Kemmner W THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling. Mod. Pathol., 2011;24(10):1390-403. 2011 [PMID: 21701537] (IHC-Fr, Human) IHC-Fr Human
Caceres M, Suwyn C, Maddox M, Thomas JW, Preuss TM Increased cortical expression of two synaptogenic thrombospondins in human brain evolution. Cereb. Cortex, 2007;17(10):2312-21. 2007 [PMID: 17182969] (Western Blot, Human) Western Blot Human

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Bioinformatics

Gene Symbol THBS4
Uniprot