1635-T2) was serially diluted and captured by Mouse Anti-Human Thrombospondin‑2 Monoclonal Antibody (Catalog # MAB16351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombospondin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1635) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Thrombospondin‑2 (Catalog # 1635-T2) was serially diluted and captured by Mouse Anti-Human Thrombospondin‑2 Monoclonal Antibody (Catalog # MAB16351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombospondin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1635) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1635-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1635-SP | |||
1635-T2) was serially diluted and captured by Mouse Anti-Human Thrombospondin‑2 Monoclonal Antibody (Catalog # MAB16351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombospondin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1635) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human Thrombospondin‑2 (Catalog # 1635-T2) was serially diluted and captured by Mouse Anti-Human Thrombospondin‑2 Monoclonal Antibody (Catalog # MAB16351) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Thrombospondin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1635) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB16351-SP | |||
| Carrier Free | MAB16351-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58202-25ul | |||
![N/A Thrombospondin-2 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Thrombospondin2_DTSP20_ELISA_860.jpg "N/A Thrombospondin-2 [HRP]")
![N/A Thrombospondin-2 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Thrombospondin2_DTSP20_ELISA_861.jpg)
![N/A Thrombospondin-2 [HRP]](http://images.novusbio.com/fullsize/elisa/Thrombospondin-2_DTSP20_ELISA_200.jpg)
![Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249342756.jpg "Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249355547.jpg)
![ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249355595.jpg)
![Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-212024941598.jpg)
![ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249413386.jpg)
![N/A Thrombospondin-2 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Thrombospondin2_DY1635_ELISA_1691.jpg "N/A Thrombospondin-2 [Biotin]")
![Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Thrombospondin 2 antibody [HL1194] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25720_rabbit-thrombospondin-2-mab-hl1194-azide-and-bsa-free-212024940883.jpg "Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Thrombospondin 2 antibody [HL1194] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25720_rabbit-thrombospondin-2-mab-hl1194-azide-and-bsa-free-2120249425478.jpg)
![Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25720_rabbit-thrombospondin-2-mab-hl1194-azide-and-bsa-free-212024939199.jpg)
![ELISA: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25720_rabbit-thrombospondin-2-mab-hl1194-azide-and-bsa-free-2120249393564.jpg)
![Western Blot: Thrombospondin-2 Antibody (HL1194) - Azide and BSA Free [NBP3-25720] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1194] (NBP3-25720) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25720_rabbit-thrombospondin-2-mab-hl1194-azide-and-bsa-free-2120249393538.jpg)
![Western Blot: Thrombospondin-2 Antibody [NBP3-13398] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody (NBP3-13398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Western-Blot-NBP3-13398-img0002.jpg "Western Blot: Thrombospondin-2 Antibody [NBP3-13398] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody (NBP3-13398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody [NBP3-13398] - Thrombospondin-2 antibody detects Thrombospondin-2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Thrombospondin-2 stained by Thrombospondin-2 antibody (NBP3-13398) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13398-img0004.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Thrombospondin-2 Antibody [NBP3-13398] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody (NBP3-13398) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Western-Blot-NBP3-13398-img0001.jpg)
![Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody [NBP3-13398] - Thrombospondin-2 antibody detects Thrombospondin-2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Thrombospondin-2 stained by Thrombospondin-2 antibody (NBP3-13398) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13398-img0003.jpg)
![Western Blot: Thrombospondin-2 Antibody (GT147) [NBP3-13654] - AsPC1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody [GT147] (NBP3-13654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-GT147-Western-Blot-NBP3-13654-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Thrombospondin-2 Antibody (GT147) [NBP3-13654] - AsPC1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody [GT147] (NBP3-13654) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody (GT147) [NBP3-13654] - Thrombospondin-2 antibody [GT147] detects Thrombospondin-2 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreas (left) and pancreatic ductal adenocarcinoma (middle and right). Thrombospondin-2 stained by Thrombospondin-2 antibody [GT147] (NBP3-13654) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-GT147-Immunohistochemistry-Paraffin-NBP3-13654-img0002.jpg)
![Western Blot: Thrombospondin-2 Antibody [NBP3-13399] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody (NBP3-13399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Western-Blot-NBP3-13399-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Thrombospondin-2 Antibody [NBP3-13399] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin-2 antibody (NBP3-13399) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody [NBP3-13399] - Thrombospondin-2 antibody detects Thrombospondin-2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Thrombospondin-2 stained by Thrombospondin-2 antibody (NBP3-13399) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13399-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody [NBP2-58202] - Staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Thrombospondin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58202-img0002.jpg "Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody [NBP2-58202] - Staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.")
