Recombinant Human S100A7 Protein, CF

Recombinant Human S100A7 (Catalog # 9085-SA) induces IL-8secretion in A431 human epithelial carcinoma cells. The ED50 forthis effect is 2-10 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human S100A7 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IL-8 secretion in A431 human epithelial carcinoma cells. The ED₅₀ for this effect is 2-10 μg/mL.
• Source: E. coli-derived human S100A7 protein
  Ser2-Gln101 with an N-terminal Met
• Accession #: AAH34687
• N-terminal Sequence: Ser2 (Major) & Met1 (Minor)
• Structure / Form: Noncovalently-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: S100A7
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 11 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human S100A7 Protein, CF

• HID 5
• HID5
• HID-5
• protein S100-A7
• PSOR1
• psoriasin 1
• Psoriasin
• S100 calcium binding protein A7 (psoriasin 1)
• S100 calcium binding protein A7
• S100 calcium-binding protein A7 (psoriasin 1)
• S100 calcium-binding protein A7
• S100A7
• S100A7c

Background

S100A7, also known as Psoriasin, is a 11-12 kDa member of the S100 family of EF hand calcium binding proteins (1). Human S100A7 shares 32% amino acid sequence identity with mouse S100A7A, the closest related protein in mouse (2). It is acetylated at the N-terminus and binds both calcium and zinc ions (3, 4). S100A7 is up-regulated in keratinocytes of psoriasis and atopic dermatitis lesions (5-9), as well as in epithelial cells of the tongue, eye, and female genital tract (10-12). Its up-regulation can be induced by bacterial exposure, inflammatory cytokines, or epidermal barrier disruption (3, 9, 11, 12). S100A7 supports epithelial integrity through killing E. coli by sequestration of zinc (3, 5) and through inducing the up-regulation of tight junction proteins (13). The interaction of S100A7 with RAGE promotes the migration of immune cells and the infiltration of macrophages into tumor sites (7, 14).

1. Donato, R. et al. (2013) Curr. Mol. Med. 13:24.
2. Madsen, P. et al. (1991) J. Invest. Dermatol. 97:701.
3. Glaser, R. et al. (2005) Nat. Immunol. 6:57.
4. Brodersen, D.E. et al. (1999) Biochemistry 38:1695.
5. Lee, K.C. and R.L. Eckert (2007) J. Invest. Dermatol. 127:945.
6. Anderson, K.S. et al. (2009) Br. J. Dermatol. 160:325.
7. Wolf, R. et al. (2008) J. Immunol. 181:1499.
8. Al-Haddad, S. et al. (1999) Am. J. Pathol. 155:2057.
9. Glaser, R. et al. (2009) J. Invest. Dermatol. 129:641.
10. Meyer, J.E. et al. (2008) Mucosal Immunol. 1:239.
11. Garreis, F. et al. (2011) Invest. Opthalmol. Vis. Sci. 52:4914.
12. Mildner, M. et al. (2010) Mucosal Immunol. 3:602.
13. Hattori, F. et al. (2014) Br. J. Dermatol. 171:742.
14. Nasser, M.W. et al. (2015) Cancer Res. 75:974.

Publications for S100A7/Psoriasin (9085-SA)(1)

Publication using 9085-SA

• MG Muoio, M Talia, R Lappano, AH Sims, V Vella, F Cirillo, L Manzella, M Giuliano, M Maggiolini, A Belfiore, EM De Frances Activation of the S100A7/RAGE Pathway by IGF-1 Contributes to Angiogenesis in Breast Cancer Cancers, 2021-02-04;13(4):. 2021-02-04 [PMID: 33557316] (Bioassay, Human)

Bioinformatics

• Gene Symbol: S100A7
• Uniprot:
  • AAH34687()