Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF


1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.

Product Details

Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity

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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
E. coli-derived CXCL12/SDF-1 alpha protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
  • Bioactivity2
Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7 kDa, reducing confitions
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350-NS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1


SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha . The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

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Publications for CXCL12/SDF-1 alpha (350-NS/CF)(167)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 9 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), ELISA Control, Enzyme Assay, Functional Assay, In Vivo, Positive Control.

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Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 167. Show All 167 Publications.
Publications using 350-NS/CF Applications Species
M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer Oncology Letters, 2022;24(5):417. 2022 [PMID: 36245829] (Bioassay, Human) Bioassay Human
S Leng, L Liu, W Xu, F Yang, J Du, L Ye, D Huang, L Zhang Inflammation down regulates stromal cell-derived factor 1alpha in the early phase of pulpitis Cytokine, 2022;158(0):155983. 2022 [PMID: 35930964] (Bioassay, Human) Bioassay Human
M Mastrogiov, P Vargas, T Rose, C Cuche, E Esposito, M Juzans, H Laude, A Schneider, M Bernard, S Goyard, C Renaudat, MN Ungeheuer, J Delon, A Alcover, V Di Bartolo The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration Science Advances, 2022;8(15):eabl5942. 2022 [PMID: 35417240] (Bioassay, Human) Bioassay Human
TA Burley, A Hesketh, G Bucca, E Kennedy, EE Ladikou, BP Towler, S Mitchell, CP Smith, C Fegan, R Johnston, A Pepper, C Pepper Elucidation of Focal Adhesion Kinase as a Modulator of Migration and Invasion and as a Potential Therapeutic Target in Chronic Lymphocytic Leukemia Cancers, 2022;14(7):. 2022 [PMID: 35406371] (Bioassay, Human) Bioassay Human
J Xu, J Hu, S Idlett-Ali, L Zhang, K Caples, S Peddibhotl, M Reeves, C Zgheib, S Malany, KW Liechty Discovery of Small Molecule Activators of Chemokine Receptor CXCR4 That Improve Diabetic Wound Healing International Journal of Molecular Sciences, 2022;23(4):. 2022 [PMID: 35216311] (Bioassay, Human) Bioassay Human
ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling Journal of leukocyte biology, 2022;0(0):. 2022 [PMID: 35137444] (Bioassay, Mouse) Bioassay Mouse
C Fielding, A García-Gar, C Korn, S Gadomski, Z Fang, JL Reguera, JA Pérez-Simó, B Göttgens, S Méndez-Fer Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis Nature Communications, 2022;13(1):543. 2022 [PMID: 35087060] (ELISA Control, Mouse) ELISA Control Mouse
T Radaszkiew, M Nosková, K Gömöryová, O Vondálová, KA Radaszkiew, M Picková, R Víchová, T Gybe?, K Kaiser, L Demková, L Ku?erová, T Bárta, D Pot?šil, Z Zdráhal, K Sou?ek, V Bryja RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy Elife, 2021;10(0):. 2021 [PMID: 34702444] (Bioassay, Human) Bioassay Human
LA Ridge, D Kewbank, D Schütz, R Stumm, PJ Scambler, S Ivins Dual role for CXCL12 signaling in semilunar valve development Cell Reports, 2021;36(8):109610. 2021 [PMID: 34433040] (Bioassay, Mouse) Bioassay Mouse
S Berrazouan, A Doucet, M Boisvert, F Barabé, F Aoudjit VLA-4 Induces Chemoresistance of T Cell Acute Lymphoblastic Leukemia Cells via PYK2-Mediated Drug Efflux Cancers, 2021;13(14):. 2021 [PMID: 34298726] (Bioassay, Human) Bioassay Human
Show All 167 Publications.

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Gene Symbol CXCL12