Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF


1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.

Product Details

Reactivity Hu, Fe, RMSpecies Glossary
Applications BA

Order Details

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
E. coli-derived
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7 kDa, reducing confitions
Read Publications using
350-NS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1


SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

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Publications for (104)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 7 applications: Binding Assay, Bioassay, Competitive Binding Assay, ELISA Standard, EnzAct, In Vivo, cell culture.

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Binding Assay
Competitive Binding Assay
ELISA Standard
In Vivo
cell culture
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Filter By Species
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 104. Show All 104 Publications.
Publications using 350-NS/CF Applications Species
XJ Li, P Liu, WW Tian, ZF Li, BG Liu, JF Sun Mechanisms of CXCR7 induction in malignant melanoma development Oncol Lett, 2017;14(4):4106-4114. 2017 [PMID: 28943917] (Bioassay, Human) Bioassay Human
S Park, H Jang, BS Kim, C Hwang, GS Jeong, Y Park Directional migration of mesenchymal stem cells under an SDF-1? gradient on a microfluidic device PLoS ONE, 2017;12(9):e0184595. 2017 [PMID: 28886159] (Bioassay, Human) Bioassay Human
X Liu, Y Liu, X Huang, G Lin, C Xie Endothelial progenitor cell dysfunction in acute exacerbation of chronic obstructive pulmonary disease Mol Med Rep, 2017;0(0):. 2017 [PMID: 28849108] (Bioassay, Human) Bioassay Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (Bioassay, Human) Bioassay Human
M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells Sci Rep, 2017;7(1):8289. 2017 [PMID: 28811579] (Bioassay, Human) Bioassay Human
M Veenstra, DW Williams, TM Calderon, K Anastos, S Morgello, JW Berman Frontline Science: CXCR7 mediates CD14(+)CD16(+) monocyte transmigration across the blood brain barrier: a potential therapeutic target for NeuroAIDS J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28754798] (Bioassay, Human) Bioassay Human
PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28637896] (Bioassay, Mouse) Bioassay Mouse
E Crompot, M Van Damme, K Pieters, M Vermeersch, D Perez-Morg, P Mineur, M Maerevoet, N Meuleman, D Bron, L Lagneaux, B Stamatopou Extracellular vesicles of bone marrow stromal cells rescue chronic lymphocytic leukemia B-cells from apoptosis, enhance their migration and induce gene expression modifications Haematologica, 2017;0(0):. 2017 [PMID: 28596280] (Bioassay, Human) Bioassay Human
JH Kim, JW Shim, DY Eum, SD Kim, SH Choi, K Yang, K Heo, MT Park Downregulation of UHRF1 increases tumor malignancy by activating the CXCR4/AKT-JNK/IL-6/Snail signaling axis in hepatocellular carcinoma cells Sci Rep, 2017;7(1):2798. 2017 [PMID: 28584306] (Bioassay, Human) Bioassay Human
R Desplantes, C Lévêque, B Muller, M Lotierzo, G Ferracci, M Popoff, M Seagar, R Mamoun, O El Far Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Sci Rep, 2017;7(1):1032. 2017 [PMID: 28432329] (Bioassay, Human) Bioassay Human
Show All 104 Publications.

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Gene Symbol CXCL12