Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

Images

 
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.

Product Details

Summary
Reactivity Hu, Fe, RMSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
Source
E. coli-derived
Lys22-Lys89
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL12
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing confitions
Publications
Read Publications using
350-NS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

Customers Who Viewed This Item Also Viewed...

MAB172
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
MAB182
Species: Hu
Applications: Flow, CyTOF-ready, ICC, Neut
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-24779
Species: Hu, Mu, Rt, Bv, Eq, Pm
Applications: WB, Simple Western, Flow, ICC/IF, IHC-P
366-6C/CF
Species: Hu
MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
AF470
Species: Mu
Applications: WB, IHC, Neut
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
NBP1-48337
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
350-NS/CF
Species: Hu, Fe, RM
Applications: BA

Publications for (104)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 7 applications: Binding Assay, Bioassay, Competitive Binding Assay, ELISA Standard, EnzAct, In Vivo, cell culture.


Filter By Application
Binding Assay
(1)
Bioassay
(84)
Competitive Binding Assay
(1)
ELISA Standard
(5)
EnzAct
(1)
In Vivo
(10)
cell culture
(2)
All Applications
Filter By Species
Human
(78)
Mouse
(14)
Rat
(4)
N/A
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 104. Show All 104 Publications.
Publications using 350-NS/CF Applications Species
XJ Li, P Liu, WW Tian, ZF Li, BG Liu, JF Sun Mechanisms of CXCR7 induction in malignant melanoma development Oncol Lett, 2017;14(4):4106-4114. 2017 [PMID: 28943917] (Bioassay, Human) Bioassay Human
S Park, H Jang, BS Kim, C Hwang, GS Jeong, Y Park Directional migration of mesenchymal stem cells under an SDF-1? gradient on a microfluidic device PLoS ONE, 2017;12(9):e0184595. 2017 [PMID: 28886159] (Bioassay, Human) Bioassay Human
X Liu, Y Liu, X Huang, G Lin, C Xie Endothelial progenitor cell dysfunction in acute exacerbation of chronic obstructive pulmonary disease Mol Med Rep, 2017;0(0):. 2017 [PMID: 28849108] (Bioassay, Human) Bioassay Human
D Kobayashi, M Endo, H Ochi, H Hojo, M Miyasaka, H Hayasaka Regulation of CCR7-dependent cell migration through�CCR7 homodimer formation Sci Rep, 2017;7(1):8536. 2017 [PMID: 28819198] (Bioassay, Human) Bioassay Human
M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells Sci Rep, 2017;7(1):8289. 2017 [PMID: 28811579] (Bioassay, Human) Bioassay Human
M Veenstra, DW Williams, TM Calderon, K Anastos, S Morgello, JW Berman Frontline Science: CXCR7 mediates CD14(+)CD16(+) monocyte transmigration across the blood brain barrier: a potential therapeutic target for NeuroAIDS J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28754798] (Bioassay, Human) Bioassay Human
PJ Stewart-Hu, TP Szasz, ER Jaeger, MD Onken, JA Cooper, SC Morley Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate J. Leukoc. Biol., 2017;0(0):. 2017 [PMID: 28637896] (Bioassay, Mouse) Bioassay Mouse
E Crompot, M Van Damme, K Pieters, M Vermeersch, D Perez-Morg, P Mineur, M Maerevoet, N Meuleman, D Bron, L Lagneaux, B Stamatopou Extracellular vesicles of bone marrow stromal cells rescue chronic lymphocytic leukemia B-cells from apoptosis, enhance their migration and induce gene expression modifications Haematologica, 2017;0(0):. 2017 [PMID: 28596280] (Bioassay, Human) Bioassay Human
JH Kim, JW Shim, DY Eum, SD Kim, SH Choi, K Yang, K Heo, MT Park Downregulation of UHRF1 increases tumor malignancy by activating the CXCR4/AKT-JNK/IL-6/Snail signaling axis in hepatocellular carcinoma cells Sci Rep, 2017;7(1):2798. 2017 [PMID: 28584306] (Bioassay, Human) Bioassay Human
R Desplantes, C Lévêque, B Muller, M Lotierzo, G Ferracci, M Popoff, M Seagar, R Mamoun, O El Far Affinity biosensors using recombinant native membrane proteins displayed on exosomes: application to botulinum neurotoxin B receptor Sci Rep, 2017;7(1):1032. 2017 [PMID: 28432329] (Bioassay, Human) Bioassay Human
Show All 104 Publications.

Reviews for CXCL12/SDF-1 alpha (0)

There are no reviews for CXCL12/SDF-1 alpha. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CXCL12/SDF-1 alpha (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CXCL12/SDF-1 alpha Products

Bioinformatics Tool for CXCL12/SDF-1 alpha

Discover related pathways, diseases and genes to CXCL12/SDF-1 alpha. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CXCL12/SDF-1 alpha

Discover more about diseases related to CXCL12/SDF-1 alpha.
 

Pathways for CXCL12/SDF-1 alpha

View related products by pathway.

PTMs for CXCL12/SDF-1 alpha

Learn more about PTMs related to CXCL12/SDF-1 alpha.

Blogs on CXCL12/SDF-1 alpha

There are no specific blogs for CXCL12/SDF-1 alpha, but you can read our latest blog posts.

Customers Who Bought This Also Bought

CXCL12/SDF-1 alpha Antibody
NBP2-29480-0.05mg

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol CXCL12
Entrez
Uniprot