Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Product Details

Summary
Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
Source
E. coli-derived CXCL12/SDF-1 alpha protein
Lys22-Lys89
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL12
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing confitions
Publications
Read Publications using
350-NS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha . The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

Publications for CXCL12/SDF-1 alpha (350-NS/CF)(174)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Feline, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 12 applications: Binding Assay, Bioassay, Cell Culture, Control, ELISA (Standard), ELISA Control, Enzyme Assay, Functional Assay, In Vivo, Migration, Positive Control, Western Blot.


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Binding Assay
(2)
Bioassay
(150)
Cell Culture
(5)
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(1)
ELISA (Standard)
(6)
ELISA Control
(1)
Enzyme Assay
(1)
Functional Assay
(1)
In Vivo
(11)
Migration
(1)
Positive Control
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(139)
Mouse
(23)
Rat
(5)
Feline
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Virus
(1)
All Species
Showing Publications 1 - 10 of 174. Show All 174 Publications.
Publications using 350-NS/CF Applications Species
Goeckel, ME;Lee, J;Levitas, A;Colijn, S;Mun, G;Burton, Z;Chintalapati, B;Yin, Y;Abello, J;Stratman, A; CXCR3-CXCL11 signaling restricts angiogenesis and promotes pericyte recruitment bioRxiv : the preprint server for biology 2023-09-17 [PMID: 37745440] (Cell Culture, Human) Cell Culture Human
YH Lai, CY Su, HW Cheng, CY Chu, LB Jeng, CS Chiang, WC Shyu, SY Chen Stem cell-nanomedicine system as a theranostic bio-gadolinium agent for targeted neutron capture cancer therapy Nature Communications, 2023-01-18;14(1):285. 2023-01-18 [PMID: 36650171] (Bioassay, Human) Bioassay Human
M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer Oncology Letters, 2022-09-30;24(5):417. 2022-09-30 [PMID: 36245829] (Bioassay, Human) Bioassay Human
X Yuan, H Wu, X Li, L Chen, Y Xiao, Z Chen, G Liu, P Lu SDF?1alpha/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathways in�vitro Molecular Medicine Reports, 2022-08-10;26(4):. 2022-08-10 [PMID: 35946444] (Bioassay, Human) Bioassay Human
H Namkoong, R Edahiro, T Takano, H Nishihara, Y Shirai, K Sonehara, H Tanaka, S Azekawa, Y Mikami, H Lee, T Hasegawa, K Okudela, D Okuzaki, D Motooka, M Kanai, T Naito, K Yamamoto, QS Wang, R Saiki, R Ishihara, Y Matsubara, J Hamamoto, H Hayashi, Y Yoshimura, N Tachikawa, E Yanagita, T Hyugaji, E Shimizu, K Katayama, Y Kato, T Morita, K Takahashi, N Harada, T Naito, M Hiki, Y Matsushita, H Takagi, R Aoki, A Nakamura, S Harada, H Sasano, H Kabata, K Masaki, H Kamata, S Ikemura, S Chubachi, S Okamori, H Terai, A Morita, T Asakura, J Sasaki, H Morisaki, Y Uwamino, K Nanki, S Uchida, S Uno, T Nishimura, T Ishiguro, T Isono, S Shibata, Y Matsui, C Hosoda, K Takano, T Nishida, Y Kobayashi, Y Takaku, N Takayanagi, S Ueda, A Tada, M Miyawaki, M Yamamoto, E Yoshida, R Hayashi, T Nagasaka, S Arai, Y Kaneko, K Sasaki, E Tagaya, M Kawana, K Arimura, K Takahashi, T Anzai, S Ito, A Endo, Y Uchimura, Y Miyazaki, T Honda, T Tateishi, S Tohda, N Ichimura, K Sonobe, CT Sassa, J Nakajima, Y Nakano, Y Nakajima, R Anan, R Arai, Y Kurihara, Y Harada, K Nishio, T Ueda, M Azuma, R Saito, T Sado, Y Miyazaki, R Sato, Y Haruta, T Nagasaki, Y Yasui, Y Hasegawa, Y Mutoh, T Kimura, T Sato, R Takei, S Hagimoto, Y Noguchi, Y Yamano, H Sasano, S Ota, Y Nakamori, K Yoshiya, F Saito, T Yoshihara, D Wada, H Iwamura, S Kanayama, S Maruyama, T Yoshiyama, K Ohta, H Kokuto, H Ogata, Y Tanaka, K Arakawa, M Shimoda, T Osawa, H Tateno, I Hase, S Yoshida, S Suzuki, M Kawada, H Horinouchi, F Saito, K Mitamura, M Hagihara, J Ochi, T Uchida, R Baba, D Arai, T Ogura, H Takahashi, S Hagiwara, G Nagao, S Konishi, I Nakachi, K Murakami, M Yamada, H Sugiura, H Sano, S Matsumoto, N Kimura, Y Ono, H Baba, Y Suzuki, S Nakayama, K Masuzawa, S Namba, K Suzuki, Y Naito, YC Liu, A Takuwa, F Sugihara, JB Wing, S Sakakibara, N Hizawa, T Shiroyama, S Miyawaki, Y Kawamura, A Nakayama, H Matsuo, Y Maeda, T Nii, Y Noda, T Niitsu, Y Adachi, T Enomoto, S Amiya, R Hara, Y Yamaguchi, T Murakami, T Kuge, K Matsumoto, Y Yamamoto, M Yamamoto, M Yoneda, T Kishikawa, S Yamada, S Kawabata, N Kijima, M Takagaki, N Sasa, Y Ueno, M Suzuki, N Takemoto, H Eguchi, T Fukusumi, T Imai, M Fukushima, H Kishima, H Inohara, K Tomono, K Kato, M Takahashi, F Matsuda, H Hirata, Y Takeda, H Koh, T Manabe, Y Funatsu, F Ito, T Fukui, K Shinozuka, S Kohashi, M Miyazaki, T Shoko, M Kojima, T Adachi, M Ishikawa, K Takahashi, T Inoue, T Hirano, K Kobayashi, H Takaoka, K Watanabe, N Miyazawa, Y Kimura, R Sado, H Sugimoto, A Kamiya, N Kuwahara, A Fujiwara, T Matsunaga, Y Sato, T Okada, Y Hirai, H Kawashima, A Narita, K Niwa, Y Sekikawa, K Nishi, M Nishitsuji, M Tani, J Suzuki, H Nakatsumi, T Ogura, H Kitamura, E Hagiwara, K Murohashi, H Okabayashi, T Mochimaru, S Nukaga, R Satomi, Y Oyamada, N Mori, T Baba, Y Fukui, M Odate, S Mashimo, Y Makino, K Yagi, M Hashiguchi, J Kagyo, T Shiomi, S Fuke, H Saito, T Tsuchida, S Fujitani, M Takita, D Morikawa, T Yoshida, T Izumo, M Inomata, N Kuse, N Awano, M Tone, A Ito, Y Nakamura, K Hoshino, J Maruyama, H Ishikura, T Takata, T Odani, M Amishima, T Hattori, Y Shichinohe, T Kagaya, T Kita, K Ohta, S Sakagami, K Koshida, K Hayashi, T Shimizu, Y Kozu, H Hiranuma, Y Gon, N Izumi, K Nagata, K Ueda, R Taki, S Hanada, K Kawamura, K Ichikado, K Nishiyama, H Muranaka, K Nakamura, N Hashimoto, K Wakahara, K Sakamoto, N Omote, A Ando, N Kodama, Y Kaneyama, S Maeda, T Kuraki, T Matsumoto, K Yokote, TA Nakada, R Abe, T Oshima, T Shimada, M Harada, T Takahashi, H Ono, T Sakurai, T Shibusawa, Y Kimizuka, A Kawana, T Sano, C Watanabe, R Suematsu, H Sageshima, A Yoshifuji, K Ito, S Takahashi, K Ishioka, M Nakamura, M Masuda, A Wakabayash, H Watanabe, S Ueda, M Nishikawa, Y Chihara, M Takeuchi, K Onoi, J Shinozuka, A Sueyoshi, Y Nagasaki, M Okamoto, S Ishihara, M Shimo, Y Tokunaga, Y Kusaka, T Ohba, S Isogai, A Ogawa, T Inoue, S Fukuyama, Y Eriguchi, A Yonekawa, K Kan-O, K Matsumoto, K Kanaoka, S Ihara, K Komuta, Y Inoue, S Chiba, K Yamagata, Y Hiramatsu, H Kai, K Asano, T Oguma, Y Ito, S Hashimoto, M Yamasaki, Y Kasamatsu, Y Komase, N Hida, T Tsuburai, B Oyama, M Takada, H Kanda, Y Kitagawa, T Fukuta, T Miyake, S Yoshida, S Ogura, S Abe, Y Kono, Y Togashi, H Takoi, R Kikuchi, S Ogawa, T Ogata, S Ishihara, A Kanehiro, S Ozaki, Y Fuchimoto, S Wada, N Fujimoto, K Nishiyama, M Terashima, S Beppu, K Yoshida, O Narumoto, H Nagai, N Ooshima, M Motegi, A Umeda, K Miyagawa, H Shimada, M Endo, Y Ohira, M Watanabe, S Inoue, A Igarashi, M Sato, H Sagara, A Tanaka, S Ohta, T Kimura, Y Shibata, Y Tanino, T Nikaido, H Minemura, Y Sato, Y Yamada, T Hashino, M Shinoki, H Iwagoe, H Takahashi, K Fujii, H Kishi, M Kanai, T Imamura, T Yamashita, M Yatomi, T Maeno, S Hayashi, M Takahashi, M Kuramochi, I Kamimaki, Y Tominaga, T Ishii, M Utsugi, A Ono, T Tanaka, T Kashiwada, K Fujita, Y Saito, M Seike, H Watanabe, H Matsuse, N Kodaka, C Nakano, T Oshio, T Hirouchi, S Makino, M Egi, Biobank Ja, Y Omae, Y Nannya, T Ueno, K Katayama, M Ai, Y Fukui, A Kumanogoh, T Sato, N Hasegawa, K Tokunaga, M Ishii, R Koike, Y Kitagawa, A Kimura, S Imoto, S Miyano, S Ogawa, T Kanai, K Fukunaga, Y Okada DOCK2 is involved in the host genetics and biology of severe COVID-19 Nature, 2022-08-08;609(7928):754-760. 2022-08-08 [PMID: 35940203] (Bioassay, Human) Bioassay Human
S Leng, L Liu, W Xu, F Yang, J Du, L Ye, D Huang, L Zhang Inflammation down regulates stromal cell-derived factor 1alpha in the early phase of pulpitis Cytokine, 2022-08-02;158(0):155983. 2022-08-02 [PMID: 35930964] (Bioassay, Human) Bioassay Human
M Mastrogiov, P Vargas, T Rose, C Cuche, E Esposito, M Juzans, H Laude, A Schneider, M Bernard, S Goyard, C Renaudat, MN Ungeheuer, J Delon, A Alcover, V Di Bartolo The tumor suppressor adenomatous polyposis coli regulates T lymphocyte migration Science Advances, 2022-04-13;8(15):eabl5942. 2022-04-13 [PMID: 35417240] (Bioassay, Human) Bioassay Human
TA Burley, A Hesketh, G Bucca, E Kennedy, EE Ladikou, BP Towler, S Mitchell, CP Smith, C Fegan, R Johnston, A Pepper, C Pepper Elucidation of Focal Adhesion Kinase as a Modulator of Migration and Invasion and as a Potential Therapeutic Target in Chronic Lymphocytic Leukemia Cancers, 2022-03-22;14(7):. 2022-03-22 [PMID: 35406371] (Bioassay, Human) Bioassay Human
J Xu, J Hu, S Idlett-Ali, L Zhang, K Caples, S Peddibhotl, M Reeves, C Zgheib, S Malany, KW Liechty Discovery of Small Molecule Activators of Chemokine Receptor CXCR4 That Improve Diabetic Wound Healing International Journal of Molecular Sciences, 2022-02-16;23(4):. 2022-02-16 [PMID: 35216311] (Bioassay, Human) Bioassay Human
ML Capitano, Y Sammour, J Ropa, M Legendre, N Mor-Vaknin, DM Markovitz DEK, a nuclear protein, is chemotactic for hematopoietic stem/progenitor cells acting through CXCR2 and Galphai signaling Journal of leukocyte biology, 2022-02-08;0(0):. 2022-02-08 [PMID: 35137444] (Bioassay, Mouse) Bioassay Mouse
Show All 174 Publications.

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Bioinformatics

Gene Symbol CXCL12
Uniprot