Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF


Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Product Details

Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity

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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
E. coli-derived CXCL12/SDF-1 alpha protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
7 kDa, reducing confitions
Read Publications using
350-NS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1


SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

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Publications for CXCL12/SDF-1 alpha (350-NS/CF)(124)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 7 applications: Binding Assay, Bioassay, Competitive Binding Assay, ELISA Standard, EnzAct, In Vivo, cell culture.

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Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 124. Show All 124 Publications.
Publications using 350-NS/CF Applications Species
W He, T Yang, XH Gong, RZ Qin, XD Zhang, WD Liu Targeting CXC motif chemokine receptor 4 inhibits the proliferation, migration and angiogenesis of lung cancer cells Oncol Lett, 2018;16(3):3976-3982. 2018 [PMID: 30128017] (Bioassay, Human) Bioassay Human
T Ohta, C Monzel, AS Becker, AD Ho, M Tanaka Simple Physical Model Unravels Influences of Chemokine on Shape Deformation and Migration of Human Hematopoietic Stem Cells Sci Rep, 2018;8(1):10630. 2018 [PMID: 30006633] (Bioassay, Human) Bioassay Human
BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
F Jahan, S Madhavan, T Rolova, L Viazmina, M Grönholm, CG Gahmberg Phosphorylation of the ?-chain in the integrin LFA-1 enables ?2-chain phosphorylation and ?-actinin binding required for cell adhesion J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29903913] (Bioassay) Bioassay
Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018;13(6):e0198740. 2018 [PMID: 29879214] (Bioassay, Human) Bioassay Human
KM Dvorak, KM Pettee, K Rubinic-Mi, R Su, A Nestor-Kal, KM Eisenmann Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2) PLoS ONE, 2018;13(3):e0195278. 2018 [PMID: 29596520] (Bioassay, Human) Bioassay Human
SYN Jamaludin, I Azimi, FM Davis, AA Peters, TJ Gonda, EW Thompson, SJ Roberts-Th, GR Monteith Assessment of CXC ligand 12-mediated calcium signalling and its regulators in basal-like breast cancer cells Oncol Lett, 2018;15(4):4289-4295. 2018 [PMID: 29541196] (Bioassay, Human) Bioassay Human
S Patalano, J Rodríguez-, C Colaneri, J Cotellessa, D Almanza, A Zhilin-Rot, T Riley, J Macoska CXCL12/CXCR4-Mediated Procollagen Secretion Is Coupled To Cullin-RING Ubiquitin Ligase Activation Sci Rep, 2018;8(1):3499. 2018 [PMID: 29472636] (Bioassay, Human) Bioassay Human
W Tang, SG Smith, W Du, A Gugilla, J Du, JP Oliveria, K Howie, BM Salter, GM Gauvreau, PM O'Byrne, R Sehmi Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma Clin Transl Allergy, 2018;8(0):5. 2018 [PMID: 29456832] (Bioassay, Human) Bioassay Human
TI Moore, J Aaron, TL Chew, TA Springer Measuring Integrin Conformational Change on the Cell Surface with Super-Resolution Microscopy Cell Rep, 2018;22(7):1903-1912. 2018 [PMID: 29444440] (Bioassay, Human) Bioassay Human
Show All 124 Publications.

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Gene Symbol CXCL12