Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

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Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha (Catalog # 350-NS/CF) chemoattracts the BaF3 mouse pro-B cells transfected with human CXCR4. The ED50 for this effect is 0.15-0.6 ng/mL.
1 μg/lane of Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Product Details

Summary
Reactivity Hu, Fe, RMSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF Summary

Details of Functionality
Measured by its ability to chemoattract 5-10 day cultured human peripheral blood lymphocytes (PBL). The ED50 for this effect is 3‑9 ng/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR4. The ED50 for this effect is 0.15‑0.6 ng/mL.
Source
E. coli-derived CXCL12/SDF-1 alpha protein
Lys22-Lys89
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CXCL12
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
7 kDa, reducing confitions
Publications
Read Publications using
350-NS/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1a, CF

  • CXCL12 chemokine (C-X-C motif) ligand 12
  • CXCL12/SDF-1 alpha
  • IRH
  • PBSF
  • SCYB12
  • SDF1 alpha
  • SDF1
  • SDF1a
  • SDF1B
  • TLSF
  • TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line.
SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a co-receptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

  1. Tashiro, K. et al. (1993) Science 261:600.
  2. Bleul, C. et al. (1996) Nature 382:829.
  3. Oberlin, E. et al. (1996) Nature 382:833.

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Applications: Bioactivity

Publications for CXCL12/SDF-1 alpha (350-NS/CF)(122)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, N/A, Primate - Macaca mulatta (Rhesus Macaque), Virus.

We have publications tested in 7 applications: Binding Assay, Bioassay, Competitive Binding Assay, ELISA Standard, EnzAct, In Vivo, cell culture.


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Binding Assay
(1)
Bioassay
(100)
Competitive Binding Assay
(1)
ELISA Standard
(5)
EnzAct
(1)
In Vivo
(11)
cell culture
(2)
All Applications
Filter By Species
Human
(93)
Mouse
(15)
Rat
(4)
N/A
(6)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
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(1)
All Species
Showing Publications 1 - 10 of 122. Show All 122 Publications.
Publications using 350-NS/CF Applications Species
BL Benson, L Li, JT Myers, RD Dorand, UA Gurkan, AY Huang, RM Ransohoff Biomimetic post-capillary venule expansions for leukocyte adhesion studies Sci Rep, 2018;8(1):9328. 2018 [PMID: 29921896]
F Jahan, S Madhavan, T Rolova, L Viazmina, M Grönholm, CG Gahmberg Phosphorylation of the ?-chain in the integrin LFA-1 enables ?2-chain phosphorylation and ?-actinin binding required for cell adhesion J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29903913] (Bioassay) Bioassay
Y Nam, SM Jung, YA Rim, H Jung, K Lee, N Park, J Kim, Y Jang, YB Park, SH Park, JH Ju Intraperitoneal infusion of mesenchymal stem cell attenuates severity of collagen antibody induced arthritis PLoS ONE, 2018;13(6):e0198740. 2018 [PMID: 29879214] (Bioassay, Human) Bioassay Human
KM Dvorak, KM Pettee, K Rubinic-Mi, R Su, A Nestor-Kal, KM Eisenmann Carcinoma associated fibroblasts (CAFs) promote breast cancer motility by suppressing mammalian Diaphanous-related formin-2 (mDia2) PLoS ONE, 2018;13(3):e0195278. 2018 [PMID: 29596520] (Bioassay, Human) Bioassay Human
SYN Jamaludin, I Azimi, FM Davis, AA Peters, TJ Gonda, EW Thompson, SJ Roberts-Th, GR Monteith Assessment of CXC ligand 12-mediated calcium signalling and its regulators in basal-like breast cancer cells Oncol Lett, 2018;15(4):4289-4295. 2018 [PMID: 29541196] (Bioassay, Human) Bioassay Human
S Patalano, J Rodríguez-, C Colaneri, J Cotellessa, D Almanza, A Zhilin-Rot, T Riley, J Macoska CXCL12/CXCR4-Mediated Procollagen Secretion Is Coupled To Cullin-RING Ubiquitin Ligase Activation Sci Rep, 2018;8(1):3499. 2018 [PMID: 29472636] (Bioassay, Human) Bioassay Human
W Tang, SG Smith, W Du, A Gugilla, J Du, JP Oliveria, K Howie, BM Salter, GM Gauvreau, PM O'Byrne, R Sehmi Interleukin-25 and eosinophils progenitor cell mobilization in allergic asthma Clin Transl Allergy, 2018;8(0):5. 2018 [PMID: 29456832] (Bioassay, Human) Bioassay Human
TI Moore, J Aaron, TL Chew, TA Springer Measuring Integrin Conformational Change on the Cell Surface with Super-Resolution Microscopy Cell Rep, 2018;22(7):1903-1912. 2018 [PMID: 29444440] (Bioassay, Human) Bioassay Human
E Vågesjö, E Öhnstedt, A Mortier, H Lofton, F Huss, P Proost, S Roos, M Phillipson Accelerated wound healing in mice by on-site production and delivery of CXCL12 by transformed lactic acid bacteria Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29432190] (In Vivo, Mouse) In Vivo Mouse
JJ Gell, J Zhao, D Chen, TJ Hunt, AT Clark PRDM14 is expressed in germ cell tumors with constitutive overexpression altering human germline differentiation and proliferation Stem Cell Res, 2018;27(0):46-56. 2018 [PMID: 29324254] (Bioassay, Human) Bioassay Human
Show All 122 Publications.

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Bioinformatics

Gene Symbol CXCL12
Entrez
Uniprot