Recombinant Human Periostin/OSF-2 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Periostin/OSF-2 Protein, CF Summary

Details of Functionality
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
Source
Mouse myeloma cell line, NS0-derived human Periostin/OSF-2 protein
Asn22-Gln836, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Asn22
Protein/Peptide Type
Recombinant Proteins
Gene
POSTN
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
91.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
85-90 kDa, reducing conditions
Publications
Read Publications using
3548-F2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Periostin/OSF-2 Protein, CF

  • Fasciclin I-like
  • MGC119510
  • MGC119511
  • OSF2
  • OSF-2
  • OSF-2osteoblast specific factor 2 (fasciclin I-like)
  • OSF2periodontal ligament-specific periostin
  • Osteoblast-specific factor 2
  • PDLPOSTN
  • periostin isoform thy2
  • periostin isoform thy4
  • periostin isoform thy6
  • periostin isoform thy8
  • Periostin
  • periostin, osteoblast specific factor
  • PNRP11-412K4.1
  • POSTN
  • TRIF52

Background

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha v beta 3 and alpha v beta 5 (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).
  1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
  2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
  3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
  4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
  5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
  6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
  7. Li, G. et al. (2006) Atherosclerosis 188:292.
  8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
  9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
  10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
  11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
  12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
  13. Snider, P. et al. (2008) Circ. Res. 102:752.
  14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
  15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
  16. Sehra, S. et al. (2011) J. Immunol. 186:4959.

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Publications for Periostin/OSF-2 (3548-F2)(18)

We have publications tested in 6 confirmed species: Human, Mouse, Canine, Deer, N/A, Red Deer.

We have publications tested in 2 applications: Bioassay, ELISA (Standard).


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Bioassay
(17)
ELISA (Standard)
(1)
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Human
(13)
Mouse
(1)
Canine
(1)
Deer
(1)
N/A
(1)
Red Deer
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using 3548-F2 Applications Species
SS Kim, GE Nikoloudak, S Michelsons, K Creber, DW Hamilton Fibronectin synthesis, but not ?-smooth muscle expression, is regulated by periostin in gingival healing through FAK/JNK signaling Sci Rep, 2019;9(1):2708. 2019 [PMID: 30804350] (Bioassay, Human) Bioassay Human
Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells Cell Rep, 2019;26(6):1533-1543.e4. 2019 [PMID: 30726736] (Bioassay, Human) Bioassay Human
Karina T Barretto, Calvin M Swanson, Christopher L Nguyen, Douglas S Annis, Stephane J Esnault, Deane F Mosher, Mats W Johansson Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin. PLoS ONE, 2018;0(0):1932-6203. 2018 [PMID: 30048528] (Bioassay, Human) Bioassay Human
KT Barretto, CM Swanson, CL Nguyen, DS Annis, SJ Esnault, DF Mosher, MW Johansson Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin PLoS ONE, 2018;13(7):e0201320. 2018 [PMID: 30048528] (Bioassay, Human) Bioassay Human
KW Yu, CC Yao, JH Jeng, HY Shieh, YJ Chen Periostin inhibits mechanical stretch-induced apoptosis in osteoblast-like MG-63�cells J. Formos. Med. Assoc., 2018;0(0):. 2018 [PMID: 29306496] (Bioassay, Human) Bioassay Human
T Mineshige, J Kamiie, G Sugahara, K Shirota A study on periostin involvement in the pathophysiology of canine atopic skin J. Vet. Med. Sci., 2017;0(0):. 2017 [PMID: 29176263] (Bioassay, Canine) Bioassay Canine
BR Kim, YW Kwon, GT Park, EJ Choi, JK Seo, IH Jang, SC Kim, HC Ko, SC Lee, JH Kim Identification of a novel angiogenic peptide from periostin PLoS ONE, 2017;12(11):e0187464. 2017 [PMID: 29095886] (Bioassay, Human) Bioassay Human
R Zhang, RR Yao, JH Li, G Dong, M Ma, QD Zheng, DM Gao, JF Cui, ZG Ren, RX Chen Activated hepatic stellate cells secrete periostin to induce stem cell-like phenotype of residual hepatocellular carcinoma cells after heat treatment Sci Rep, 2017;7(1):2164. 2017 [PMID: 28526827] (Bioassay, Human) Bioassay Human
MW Johansson, M Khanna, V Bortnov, DS Annis, CL Nguyen, DF Mosher IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration Clin. Exp. Allergy, 2017;0(0):. 2017 [PMID: 28378503] (Bioassay, Human) Bioassay Human
T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization Mol Ther Nucleic Acids, 2017;6(0):279-289. 2017 [PMID: 28325294] (Bioassay, Human) Bioassay Human
Show All 18 Publications.

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Bioinformatics

Gene Symbol POSTN
Uniprot