Recombinant Human Periostin/OSF-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Periostin/OSF-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. Immobilized Recombinant Human Periostin/OSF‑2 at 10 µg/mL (100 µL/well) induces >50% cell adhesion.
• Source: Mouse myeloma cell line, NS0-derived human Periostin/OSF-2 protein
  Asn22-Gln836, with a C-terminal 6-His tag
• Accession #: Q15063
• N-terminal Sequence: Asn22
• Protein/Peptide Type: Recombinant Proteins
• Gene: POSTN
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 91.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Periostin/OSF-2 Protein, CF

• Fasciclin I-like
• MGC119510
• MGC119511
• OSF2
• OSF-2
• OSF-2osteoblast specific factor 2 (fasciclin I-like)
• OSF2periodontal ligament-specific periostin
• Osteoblast-specific factor 2
• PDLPOSTN
• periostin isoform thy2
• periostin isoform thy4
• periostin isoform thy6
• periostin isoform thy8
• Periostin
• periostin, osteoblast specific factor
• PNRP11-412K4.1
• POSTN
• TRIF52

Background

Periostin, also known as OSF-2, is a secreted matricellular protein with functions in extracellular matrix formation, cell migration, and inflammation (1). It is secreted as a 90 kDa monomer that can aggregate into >170 kDa higher-order multimers (2). Periostin contains an N-terminal EMI domain followed by four tandem FAS1 domains (3). Mature human Periostin shares 91% aa sequence identity with mouse and rat Periostin. Alternative splicing generates additional isoforms with various deletions in the C-terminal region following the FAS domains. Periostin is expressed by mesenchymal cells such as vascular smooth muscle cells, fibroblasts, osteoblasts, and odontoblasts in developing teeth (4-7). It is up-regulated in many carcinomas (2, 8). Periostin binds to Integrins alpha _v beta ₃ and alpha _v beta ₅ (2, 9), leading to enhanced cell adhesion and cell migration (2, 5, 6). It enhances Fibronectin and Collagen I production and promotes collagen fibrillogenesis (10, 11). It also induces epithelial-mesenchymal transition, tumor growth, invasion, and metastasis (9). Periostin induces the expression of VEGF R2 on endothelial cells and VEGF-C in tumor cells, and it can induce tumor lymphangiogenesis (8, 12). Periostin plays an important role in heart valve development and tissue healing after myocardial infarction (5, 13, 14). In asthma, it is up-regulated in bronchial epithelium and plays both destructive and protective roles by inducing eosinophil infiltration and inhibiting goblet cell metaplasia and mucus production, respectively (15, 16).

1. Liu, A.Y. et al. (2014) Matrix Biol. 37:150.
2. Gillan, L. et al. (2002) Cancer Res. 62:5358.
3. Takeshita, S. et al. (1993) Biochem. J. 294:271.
4. Kruzynska-Frejtag, A. et al. (2004) Dev. Dyn. 229:857.
5. Lindner, V. et al. (2005) Arterioscler. Thromb. Vasc. Biol. 25:77.
6. Horiuchi, K. et al. (1999) J. Bone Miner. Res. 14:1239.
7. Li, G. et al. (2006) Atherosclerosis 188:292.
8. Shao, R. et al. (2004) Mol. Cell. Biol. 24:3992.
9. Yan, W. and R. Shao (2006) J. Biol. Chem. 281:19700.
10. Erkan, M. et al. (2007) Gastroenterology 132:1447.
11. Norris, R.A. et al. (2007) J. Cell. Biochem. 101:695.
12. Kudo, Y. et al. (2012) PLoS One 7:e44488.
13. Snider, P. et al. (2008) Circ. Res. 102:752.
14. Kuhn, B. et al. (2007) Nat. Med. 13:962.
15. Blanchard, C. et al. (2008) Mucosal Immunol. 1:289.
16. Sehra, S. et al. (2011) J. Immunol. 186:4959.

Publications for Periostin/OSF-2 (3548-F2)(31)

Publications using 3548-F2

• Jin, X;Deng, Q;Ye, S;Liu, S;Fu, Y;Liu, Y;Wu, G;Ouyang, G;Wu, T; Cancer-associated fibroblast-derived periostin promotes papillary thyroid tumor growth through integrin-FAK-STAT3 signaling Theranostics 2024-05-13 [PMID: 38773979] (Bioassay, Human)
• Liu, B;Wu, T;Lin, B;Liu, X;Liu, Y;Song, G;Fan, C;Ouyang, G; Periostin-TGF-? feedforward loop contributes to tumour-stroma crosstalk in liver metastatic outgrowth of colorectal cancer British journal of cancer 2023-12-14 [PMID: 38097742] (Bioassay, Human)
• Hirata, R;Iwata, T;Fujita, T;Nagahara, T;Matsuda, S;Sasaki, S;Taniguchi, Y;Hamamoto, Y;Ouhara, K;Kudo, Y;Kurihara, H;Mizuno, N; Periostin regulates integrin expression in gingival epithelial cells Journal of oral biosciences 2023-12-02 [PMID: 38048847] (Bioassay, Human)
• Suzuki, M;Ototake, Y;Akita, A;Asami, M;Ikeda, N;Watanabe, T;Kanaoka, M;Yamaguchi, Y; Periostin-An inducer of pro-fibrotic phenotype in monocytes and monocyte-derived macrophages in systemic sclerosis PloS one 2023-08-02 [PMID: 37531393] (Bioassay, Human)
• L Pang, M Dunterman, W Xuan, A Gonzalez, Y Lin, WH Hsu, F Khan, RS Hagan, WA Muller, AB Heimberger, P Chen Circadian regulator CLOCK promotes tumor angiogenesis in glioblastoma Cell Reports, 2023-02-14;42(2):112127. 2023-02-14 [PMID: 36795563] (Bioassay, Human)
• I Alesutan, LA Henze, B Boehme, TTD Luong, D Zickler, B Pieske, KU Eckardt, A Pasch, J Voelkl Periostin Augments Vascular Smooth Muscle Cell Calcification via beta-Catenin Signaling Biomolecules, 2022-08-21;12(8):. 2022-08-21 [PMID: 36009051] (Bioassay, Human)
• M Martín-Alo, S Iqbal, PM Vornewald, HT Lindholm, MJ Damen, F Martínez, S Hoel, A Díez-Sánch, M Altelaar, P Katajisto, AG Arroyo, MJ Oudhoff Smooth muscle-specific MMP17 (MT4-MMP) regulates the intestinal stem cell niche and regeneration after damage Nature Communications, 2021-11-18;12(1):6741. 2021-11-18 [PMID: 34795242] (Bioassay, Human)
• H Yamato, K Kimura, E Fukui, T Kanou, N Ose, S Funaki, M Minami, Y Shintani Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer Scientific Reports, 2021-10-26;11(1):21114. 2021-10-26 [PMID: 34702952] (Bioassay, Human)
• K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 2021-07-02;22(13):. 2021-07-02 [PMID: 34281240] (Bioassay, Human)
• Y Ikeda-Iwab, Y Taniyama, N Katsuragi, F Sanada, N Koibuchi, K Shibata, K Shimazu, H Rakugi, R Morishita Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis Cells, 2021-04-14;10(4):. 2021-04-14 [PMID: 33919736] (Surface Plasmon Resonance, Human)
• T Yoshihara, Y Nanri, S Nunomura, Y Yamaguchi, C Feghali-Bo, K Ajito, S Murakami, M Mawatari, K Izuhara Periostin plays a critical role in the cell cycle in lung fibroblasts Respir. Res., 2020-01-30;21(1):38. 2020-01-30 [PMID: 32000779] (Bioassay, Human)
• H Ma, J Wang, X Zhao, T Wu, Z Huang, D Chen, Y Liu, G Ouyang Periostin Promotes Colorectal Tumorigenesis through Integrin-FAK-Src Pathway-Mediated YAP/TAZ Activation Cell Rep, 2020-01-21;30(3):793-806.e6. 2020-01-21 [PMID: 31968254] (Bioassay, Human)
• J Ono, M Takai, A Kamei, S Nunomura, Y Nanri, T Yoshihara, S Ohta, K Yasuda, SJ Conway, Y Yokosaki, K Izuhara Periostin forms a functional complex with IgA in human serum Allergol Int, 2019-07-02;0(0):. 2019-07-02 [PMID: 31272904] (Bioassay, Human)
• SS Kim, GE Nikoloudak, S Michelsons, K Creber, DW Hamilton Fibronectin synthesis, but not ?-smooth muscle expression, is regulated by periostin in gingival healing through FAK/JNK signaling Sci Rep, 2019-02-25;9(1):2708. 2019-02-25 [PMID: 30804350] (Bioassay, Human)
• Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells Cell Rep, 2019-02-05;26(6):1533-1543.e4. 2019-02-05 [PMID: 30726736] (Bioassay, Human)
• Karina T Barretto, Calvin M Swanson, Christopher L Nguyen, Douglas S Annis, Stephane J Esnault, Deane F Mosher, Mats W Johansson Control of cytokine-driven eosinophil migratory behavior by TGF-beta-induced protein (TGFBI) and periostin. PLoS ONE, 2018-07-26;0(0):1932-6203. 2018-07-26 [PMID: 30048528] (Bioassay, Human)
• KW Yu, CC Yao, JH Jeng, HY Shieh, YJ Chen Periostin inhibits mechanical stretch-induced apoptosis in osteoblast-like MG-63�cells J. Formos. Med. Assoc., 2018-01-03;0(0):. 2018-01-03 [PMID: 29306496] (Bioassay, Human)
• T Mineshige, J Kamiie, G Sugahara, K Shirota A study on periostin involvement in the pathophysiology of canine atopic skin J. Vet. Med. Sci., 2017-11-27;0(0):. 2017-11-27 [PMID: 29176263] (Bioassay, Canine)
• BR Kim, YW Kwon, GT Park, EJ Choi, JK Seo, IH Jang, SC Kim, HC Ko, SC Lee, JH Kim Identification of a novel angiogenic peptide from periostin PLoS ONE, 2017-11-02;12(11):e0187464. 2017-11-02 [PMID: 29095886] (Bioassay, Human)
• R Zhang, RR Yao, JH Li, G Dong, M Ma, QD Zheng, DM Gao, JF Cui, ZG Ren, RX Chen Activated hepatic stellate cells secrete periostin to induce stem cell-like phenotype of residual hepatocellular carcinoma cells after heat treatment Sci Rep, 2017-05-19;7(1):2164. 2017-05-19 [PMID: 28526827] (Bioassay, Human)
• MW Johansson, M Khanna, V Bortnov, DS Annis, CL Nguyen, DF Mosher IL-5-stimulated eosinophils adherent to periostin undergo stereotypic morphological changes and ADAM8-dependent migration Clin. Exp. Allergy, 2017-05-05;0(0):. 2017-05-05 [PMID: 28378503] (Bioassay, Human)
• T Nakama, S Yoshida, K Ishikawa, Y Kubo, Y Kobayashi, Y Zhou, S Nakao, T Hisatomi, Y Ikeda, K Takao, K Yoshikawa, A Matsuda, J Ono, S Ohta, K Izuhara, A Kudo, KH Sonoda, T Ishibashi Therapeutic Effect of Novel Single-Stranded RNAi Agent Targeting Periostin in Eyes with Retinal Neovascularization Mol Ther Nucleic Acids, 2017-02-09;6(0):279-289. 2017-02-09 [PMID: 28325294] (Bioassay, Human)
• F Zhang, K Luo, Z Rong, Z Wang, F Luo, Z Zhang, D Sun, S Dong, J Xu, F Dai Periostin Upregulates Wnt/?-Catenin Signaling to Promote the Osteogenesis of CTLA4-Modified Human Bone Marrow-Mesenchymal Stem Cells Sci Rep, 2017-01-27;7(0):41634. 2017-01-27 [PMID: 28128364] (Bioassay, Human)
• Manuel Nieto-Diaz Identification of axon growth promoters in the secretome of the deer antler velvet Neuroscience, 2016-11-04;0(0):. 2016-11-04 [PMID: 27818162] (Bioassay, Deer, Rat, Red Deer)
• Soon Young Park, Yuji Piao, Kang Jin Jeong, Jianwen Dong, John F. de Groot Periostin (POSTN) Regulates Tumor Resistance to Antiangiogenic Therapy in Glioma Models Molecular Cancer Therapeutics 2016-09-01 [PMID: 27307601] (Bioassay, Human)
• Fukuda K, Sugihara E, Ohta S, Izuhara K, Funakoshi T, Amagai M, Saya H Periostin Is a Key Niche Component for Wound Metastasis of Melanoma. PLoS ONE, 2015-06-17;10(6):e0129704. 2015-06-17 [PMID: 26083413] (Bioassay, Human, Mouse)
• Ryner L, Guan Y, Firestein R, Xiao Y, Choi Y, Rabe C, Lu S, Fuentes E, Huw L, Lackner M, Fu L, Amler L, Bais C, Wang Y Upregulation of Periostin and Reactive Stroma Is Associated with Primary Chemoresistance and Predicts Clinical Outcomes in Epithelial Ovarian Cancer. Clin Cancer Res, 2015-04-02;21(13):2941-51. 2015-04-02 [PMID: 25838397] (Bioassay, Human)
• Wenchao Zhou, Susan Q. Ke, Zhi Huang, William Flavahan, Xiaoguang Fang, Jeremy Paul et al. Periostin secreted by glioblastoma stem cells recruits M2 tumour-associated macrophages and promotes malignant growth Nature Cell Biology 2015-02-01 [PMID: 25580734] (Bioassay, Human)
• Mael-Ainin M, Abed A, Conway S, Dussaule J, Chatziantoniou C Inhibition of periostin expression protects against the development of renal inflammation and fibrosis. J Am Soc Nephrol, 2014-02-27;25(8):1724-36. 2014-02-27 [PMID: 24578131] (Bioassay, Human)
• Lee Y, Kim I, Park S, Kim Y, Lee J, Noh D, Kim K, Ryu S, Suh P Periostin-binding DNA aptamer inhibits breast cancer growth and metastasis. Mol Ther, 2013-03-19;21(5):1004-13. 2013-03-19 [PMID: 23511245] (Bioassay, Human)
• Ben QW, Zhao Z, Ge SF, Zhou J, Yuan F, Yuan YZ Circulating levels of periostin may help identify patients with more aggressive colorectal cancer. Int. J. Oncol., 2009-03-01;34(3):821-8. 2009-03-01 [PMID: 19212687] (ELISA (Standard))

Bioinformatics

• Gene Symbol: POSTN
• Uniprot:
  • Q15063()