PDXP Antibody - Azide and BSA Free

Western Blot: PDXP Antibody [NBP2-93574] - Analysis of extracts of various cell lines, using PDXP at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.

Immunocytochemistry/Immunofluorescence: PDXP Antibody [NBP2-93574] - Immunofluorescence analysis of L929 cells using PDXP Rabbit pAb (NBP2-93574) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: PDXP Antibody [NBP2-93574] - Immunohistochemistry of paraffin-embedded Human colon using PDXP Rabbit pAb (NBP2-93574) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: PDXP Antibody [NBP2-93574] - Immunohistochemistry of paraffin-embedded Mouse kidney using PDXP Rabbit pAb (NBP2-93574) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: PDXP Antibody [NBP2-93574] - Immunohistochemistry of paraffin-embedded Rat lung using PDXP Rabbit pAb (NBP2-93574) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PDXP Antibody - Azide and BSA Free Summary

• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human PDXP (NP_064711.1). VETASGRQALVVGKPSPYMFECITENFSIDPARTLMVGDRLETDILFGHRCGMTTVLTLTGVSRLEEAQAYLAAGQHDLVPHYYVESIADLTEGLED
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDXP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for PDXP Antibody - Azide and BSA Free

• chronophin
• CIN
• dJ37E16.5
• EC 3.1.3
• EC 3.1.3.74
• FLJ32703
• PLP phosphatase
• PLP
• PLPP
• pyridoxal (pyridoxine, vitamin B6) phosphatase
• pyridoxal phosphate phosphatase

Background

Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP (Jang et al., 2003 (PubMed 14522954)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: PDXP