Novus Biologicals products are now on

Recombinant Human NT-4 Protein, CF


There are currently no images for NT-4 (268-N4/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human NT-4 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.3‑3 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-4 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
268-N4/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-4 Protein, CF

  • GLC10
  • GLC1O
  • neurotrophic factor 4
  • neurotrophic factor 5
  • neurotrophin 4
  • neurotrophin 5 (neurotrophin 4/5)
  • neurotrophin-4
  • neurotrophin-5
  • Neutrophic factor 4
  • NT4
  • NT-4
  • NT-4/5
  • NT-5
  • NTF4
  • NTF5
  • NTF5NT5


Neurotrophin-4 (NT-4), also known as NT-5, is a member of the NGF family of neuronal and epithelial growth factors. Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds (1-3). The human NT-4 cDNA encodes a 210 amino acid (aa) precursor that includes a 24 aa signal sequence, a 56 aa propeptide, and a 130 aa mature protein (4, 5). NT-4 is synthesized as a 28 kDa prepropeptide that is proteolytically processed to generate the mature protein. Mature human NT-4 shares 48-52% aa sequence identity with human beta-NGF, BDNF, and NT-3. It shares 91% and 95% aa sequence identity with mouse and rat NT-4/5, respectively. The mature protein is secreted as a homodimer and can also form heterodimers with BDNF or NT-3 (6). NT-4 binds and induces receptor dimerization and activation of TrkB (4, 7). NT-4 promotes the development and survival of selected peripheral and CNS neurons (8-10). BDNF, which also activates TrkB, overlaps with many but not all NT-4 functions, a distinction that is likely due to differences in expression patterns (8-10). NT-4 induced TrkB signaling augments NMDA receptor activity and increases neuronal sensitivity to excitotoxic cell death (11). It also promotes the proliferation of keratinocytes and accelerates hair follicle regression during the follicular cycle (12, 13). NT-4 is secreted by activated T cells and granulocytes at sites of inflammation where it contributes to tissue regeneration (14-16).

  1. Lessmann, V. et al. (2003) Prog. Neurobiol. 69:341.
  2. Tabakman, R. et al. (2004) Prog. Brain Res. 146:387.
  3. Botchkarev, V.A. et al. (2004) Prog. Brain Res. 146:493.
  4. Ip, N.Y. et al. (1992) Proc. Natl. Acad. Sci. 89:3060.
  5. Berkemeier, L.R. et al. (1991) Neuron 7:857.
  6. Radziejewski, C. and R.C. Robinson (1993) Biochemistry 32:13350.
  7. Vesa, J. et al. (2000) J. Biol. Chem. 275:24414.
  8. Davies, A.M. et al. (1993) J. Neurosci. 13:4961.
  9. Stucky, C.L. et al. (1998) J. Neurosci. 18:7040.
  10. Fan, G. et al. (2000) Nat. Neurosci. 3:350.
  11. Choi, S.Y. et al. (2004) J. Neurochem. 88:708.
  12. Botchkarev, V.A. et al. (1999) Lab. Invest. 79:557.
  13. Botchkarev, V.A. et al. (1999) FASEB J. 13:395.
  14. Laurenzi, M.A. et al. (1998) J. Leukoc. Biol. 64:228.
  15. Moalem, G. et al. (2000) J. Autoimmun. 15:331.
  16. Nassenstein, C. et al. (2003) J. Exp. Med. 198:455.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB
Species: Rt
Applications: IHC, WB
Species: Mu
Applications: IHC, WB
Species: Mu, Rt
Applications: Block, IHC, Simple Western, WB
Species: Hu
Applications: ELISA, IHC, IHC-P, S-ELISA, WB
Species: Hu
Applications: BA
Species: Hu
Applications: Bind, BA
Species: Hu
Applications: Neut, WB
Species: Hu
Applications: ICC, WB
Species: Hu
Applications: IHC, IHC-P
Species: Mu
Applications: Block, IHC, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: ELISA, IHC, IHC-P, KD, PLA, S-ELISA, WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: Bioactivity

Publications for NT-4 (268-N4/CF)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.

Filter By Application
Cell Culture
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 9 of 9.
Publications using 268-N4/CF Applications Species
Y Tian, W Wang, S Lautrup, H Zhao, X Li, PWN Law, ND Dinh, EF Fang, HH Cheung, WY Chan WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome Nature Communications, 2022-09-16;13(1):5456. 2022-09-16 [PMID: 36114168] (Bioassay, Human) Bioassay Human
D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021-12-21;37(12):110152. 2021-12-21 [PMID: 34936870] (Bioassay, Mouse) Bioassay Mouse
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020-08-21;9(9):. 2020-08-21 [PMID: 32839392] (Bioassay, Cell Culture, Human) Bioassay, Cell Culture Human
N Michel, P Narayanan, O Shomroni, M Schmidt Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction Cell Rep, 2020-07-21;32(3):107912. 2020-07-21 [PMID: 32697985] (Bioassay, Mouse) Bioassay Mouse
P Teotia, M Niu, I Ahmad Mapping developmental trajectories and subtype diversity of normal and glaucomatous human retinal ganglion cells by single-cell transcriptome analysis Stem Cells, 2020-06-18;0(0):. 2020-06-18 [PMID: 32557945] (Bioassay, Human) Bioassay Human
M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo Int J Nanomedicine, 2019-08-01;14(0):6117-6131. 2019-08-01 [PMID: 31534337] (In Vivo, Mouse) In Vivo Mouse
Samah B, Porcheray F, Gras G Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 2008-01-08;151(3):476-86. 2008-01-08 [PMID: 18190610] (Bioassay, Human) Bioassay Human
Royo NC, Lebold D, Magge SN, Chen I, Hauspurg A, Cohen AS, Watson DJ Neurotrophin-mediated neuroprotection of hippocampal neurons following traumatic brain injury is not associated with acute recovery of hippocampal function. Neuroscience, 2007-08-02;148(2):359-70. 2007-08-02 [PMID: 17681695] (In Vivo, Rat) In Vivo Rat
Getmanova EV, Chen Y, Bloom L, Gokemeijer J, Shamah S, Warikoo V, Wang J, Ling V, Sun L Antagonists to human and mouse vascular endothelial growth factor receptor 2 generated by directed protein evolution in vitro. Chem. Biol., 2006-05-01;13(5):549-56. 2006-05-01 [PMID: 16720276] (Bioassay, Mouse) Bioassay Mouse

Reviews for NT-4 (268-N4/CF) (0)

There are no reviews for NT-4 (268-N4/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for NT-4 (268-N4/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional NT-4 Products

Blogs on NT-4

There are no specific blogs for NT-4, but you can read our latest blog posts.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human NT-4 Protein, CF and receive a gift card or discount.


Gene Symbol NTF4